Angiogenesis is a crucial process in the development of inflammatory diseases,including cancer,psoriasis and rheumatoid arthritis.Recently,several alkaloids from Picrasma quassioides had been screened for angiogenic a...Angiogenesis is a crucial process in the development of inflammatory diseases,including cancer,psoriasis and rheumatoid arthritis.Recently,several alkaloids from Picrasma quassioides had been screened for angiogenic activity in the zebrafish model,and the results indicated that 1-methoxycarbony-β-carboline(MCC) could effectively inhibit blood vessel formation.In this study,we further confirmed that MCC can inhibit,in a concentration-dependent manner,the viability,migration,invasion,and tube formation of human umbilical vein endothelial cells(HUVECs) in vitro,as well as the regenerative vascular outgrowth of zebrafish caudal fin in vivo.In the zebrafish xenograft assay,MCC inhibited the growth of tumor masses and the metastatic transplanted DU145 tumor cells.The proteome profile array of the MCC-treated HUVECs showed that MCC could down-regulate several angiogenesis-related self-secreted proteins,including ANG,EGF,b FGF,GRO,IGF-1,PLG and MMP-1.In addition,the expression of two key membrane receptor proteins in angiogenesis,TIE-2 and u PAR,were also down-regulated after MCC treatment.Taken together,these results shed light on the potential therapeutic application of MCC as a potent natural angiogenesis inhibitor via multiple molecular targets.展开更多
【目的】探究2型糖尿病伴视网膜病变患者不同中医证型与发生心律失常风险的关系。【方法】选取137例2型糖尿病伴视网膜病变患者的临床资料,根据中医证型分为气阴两虚证38例、肝肾亏虚证47例和阴阳两虚证52例。回顾性分析不同中医证型患...【目的】探究2型糖尿病伴视网膜病变患者不同中医证型与发生心律失常风险的关系。【方法】选取137例2型糖尿病伴视网膜病变患者的临床资料,根据中医证型分为气阴两虚证38例、肝肾亏虚证47例和阴阳两虚证52例。回顾性分析不同中医证型患者的血脂指标[总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)]、心率变异性时域参数[全部窦性心搏N-N间期标准差(SDNN)、全程记录中每5 min N-N间期平均值的标准差(SDANN)、全程相邻N-N间期差值的均方根(RMSSD)、相邻R-R间期相差≥50 ms占总窦性心律的百分数(PNN50)]及心律失常类型[室上性心律失常、室性心律失常、传导阻滞、ST-T改变]的差异。【结果】(1)血脂方面:在糖尿病伴视网膜病变患者的中医证型中,阴阳两虚证的TC和LDL-C水平高于其他2组证型(P<0.01),而3组证型的HDL-C和TG比较,差异无统计学意义(P>0.05)。(2)心率变异性方面:阴阳两虚证的PNN50、SDANN、SDNN低于其他2组证型(P<0.05或P<0.01),而肝肾亏虚证组的PNN50和SDNN低于气阴两虚证组(P<0.05或P<0.01),气阴两虚证的RMSSD低于其他2组证型(P<0.01),其余各组两两比较差异无统计学意义(P>0.05)。(3)心律失常方面:阴阳两虚证的心律失常发生率明显高于气阴两虚证和肝肾亏虚证,差异均有统计学意义(P<0.01);而气阴两虚证与肝肾亏虚证比较,差异无统计学意义(P>0.05)。【结论】糖尿病伴视网膜病变患者发生心律失常与不同中医证型有一定相关性。展开更多
The present study was designed to determine the major chemical constituents of the leaves of Rhododendron dauricum L. Compounds were isolated and purified by various chromatographic methods, and their structures were ...The present study was designed to determine the major chemical constituents of the leaves of Rhododendron dauricum L. Compounds were isolated and purified by various chromatographic methods, and their structures were elucidated by physicochemical properties and spectral data. The present study identified two new C-methyl flavanones, 5, 7, 3', 5'-tetrahydroxy-6, 8-di-C-methyl flavanone(1) and 5, 4'-dihydroxy-8-C-methylflavanone-7-O-β-D-glucopyranoside(2), and one new flavonoid glycoside, quercetin-3-O-β-D-(6''-O-cinnamoyl)-galactoside(3), along with seven known compounds, including syzalterin(4), poriolin(5), farrerol-7-O-β-D-glucopyranoside(6), myrciacetin(7), quercetin-3-O-β-D-(6-p-hydroxy-benzoyl)-galactoside(8), quercetin-3-O-β-D-(6-p-coumaroyl)-galactoside(9), and 5, 7, 3', 5'-tetrahydroxyl flavanone(10). Compounds 1-3 were determined to be new flavonoids; compounds 4-6 were isolated from this species for the first time; and compounds 7-10 were reported for the first time from this genus.展开更多
基金supported by the National Natural Science Foundation of China(No.81502950)the Fundamental Research Funds for the Central Universities(2015ZD010)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Angiogenesis is a crucial process in the development of inflammatory diseases,including cancer,psoriasis and rheumatoid arthritis.Recently,several alkaloids from Picrasma quassioides had been screened for angiogenic activity in the zebrafish model,and the results indicated that 1-methoxycarbony-β-carboline(MCC) could effectively inhibit blood vessel formation.In this study,we further confirmed that MCC can inhibit,in a concentration-dependent manner,the viability,migration,invasion,and tube formation of human umbilical vein endothelial cells(HUVECs) in vitro,as well as the regenerative vascular outgrowth of zebrafish caudal fin in vivo.In the zebrafish xenograft assay,MCC inhibited the growth of tumor masses and the metastatic transplanted DU145 tumor cells.The proteome profile array of the MCC-treated HUVECs showed that MCC could down-regulate several angiogenesis-related self-secreted proteins,including ANG,EGF,b FGF,GRO,IGF-1,PLG and MMP-1.In addition,the expression of two key membrane receptor proteins in angiogenesis,TIE-2 and u PAR,were also down-regulated after MCC treatment.Taken together,these results shed light on the potential therapeutic application of MCC as a potent natural angiogenesis inhibitor via multiple molecular targets.
文摘【目的】探究2型糖尿病伴视网膜病变患者不同中医证型与发生心律失常风险的关系。【方法】选取137例2型糖尿病伴视网膜病变患者的临床资料,根据中医证型分为气阴两虚证38例、肝肾亏虚证47例和阴阳两虚证52例。回顾性分析不同中医证型患者的血脂指标[总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)]、心率变异性时域参数[全部窦性心搏N-N间期标准差(SDNN)、全程记录中每5 min N-N间期平均值的标准差(SDANN)、全程相邻N-N间期差值的均方根(RMSSD)、相邻R-R间期相差≥50 ms占总窦性心律的百分数(PNN50)]及心律失常类型[室上性心律失常、室性心律失常、传导阻滞、ST-T改变]的差异。【结果】(1)血脂方面:在糖尿病伴视网膜病变患者的中医证型中,阴阳两虚证的TC和LDL-C水平高于其他2组证型(P<0.01),而3组证型的HDL-C和TG比较,差异无统计学意义(P>0.05)。(2)心率变异性方面:阴阳两虚证的PNN50、SDANN、SDNN低于其他2组证型(P<0.05或P<0.01),而肝肾亏虚证组的PNN50和SDNN低于气阴两虚证组(P<0.05或P<0.01),气阴两虚证的RMSSD低于其他2组证型(P<0.01),其余各组两两比较差异无统计学意义(P>0.05)。(3)心律失常方面:阴阳两虚证的心律失常发生率明显高于气阴两虚证和肝肾亏虚证,差异均有统计学意义(P<0.01);而气阴两虚证与肝肾亏虚证比较,差异无统计学意义(P>0.05)。【结论】糖尿病伴视网膜病变患者发生心律失常与不同中医证型有一定相关性。
基金supported by the National Natural Science Foundation of China(No.81373956)
文摘The present study was designed to determine the major chemical constituents of the leaves of Rhododendron dauricum L. Compounds were isolated and purified by various chromatographic methods, and their structures were elucidated by physicochemical properties and spectral data. The present study identified two new C-methyl flavanones, 5, 7, 3', 5'-tetrahydroxy-6, 8-di-C-methyl flavanone(1) and 5, 4'-dihydroxy-8-C-methylflavanone-7-O-β-D-glucopyranoside(2), and one new flavonoid glycoside, quercetin-3-O-β-D-(6''-O-cinnamoyl)-galactoside(3), along with seven known compounds, including syzalterin(4), poriolin(5), farrerol-7-O-β-D-glucopyranoside(6), myrciacetin(7), quercetin-3-O-β-D-(6-p-hydroxy-benzoyl)-galactoside(8), quercetin-3-O-β-D-(6-p-coumaroyl)-galactoside(9), and 5, 7, 3', 5'-tetrahydroxyl flavanone(10). Compounds 1-3 were determined to be new flavonoids; compounds 4-6 were isolated from this species for the first time; and compounds 7-10 were reported for the first time from this genus.
