目的观察针刺配合口服真武汤治疗原发性慢性肾病的临床疗效。方法将90例原发性慢性肾病患者随机分为A组、B组和C组,每组30例。A组采用常规营养及对症治疗,B组在A组基础上采用口服真武汤治疗,C组在A组基础上采用针刺配合口服真武汤治疗...目的观察针刺配合口服真武汤治疗原发性慢性肾病的临床疗效。方法将90例原发性慢性肾病患者随机分为A组、B组和C组,每组30例。A组采用常规营养及对症治疗,B组在A组基础上采用口服真武汤治疗,C组在A组基础上采用针刺配合口服真武汤治疗。观察3组治疗前后各项生化指标[血清肌酐(SCr)、血尿素氮(BUN)、血尿酸(UA)、24 h尿蛋白定量(24 h UPro)、肾小球滤过率(GFR)水平]及右肾皮质、髓质各项血流量灌注指标[皮质到达时间(AT)、达峰值时间(PT)、峰值强度(PI)、上升支斜率(A)、曲线下面积(AUC)]的变化情况,并比较3组临床疗效。结果B组和C组治疗后各项生化指标及右肾皮质、髓质各项血流量灌注指标(AT、PT、PI、AUC)与同组治疗前比较,差异均具有统计学意义(P<0.05)。A组治疗后SCr、24 h UPro、GFR指标及右肾皮质、髓质PT与同组治疗前比较,差异均具有统计学意义(P<0.05)。B组和C组治疗后BUN指标及右肾皮质、髓质AT、PT与A组比较,差异均具有统计学意义(P<0.05)。A组总有效率为66.7%,B组为86.7%,C组为93.3%。C组总有效率与A组和B组比较,差异均具有统计学意义(P<0.05);B组总有效率与A组比较,差异具有统计学意义(P<0.05)。结论针刺配合口服真武汤是一种治疗原发性慢性肾病的有效方法,能改善患者肾功能及肾血流灌注状态。展开更多
Background Cystic echinococcosis due to Echinococcus granulosus （E. granulosus） is one of the most important chronic helminthic diseases, especially in sheep/cattle-raising regions. The larval stage of the parasite ...Background Cystic echinococcosis due to Echinococcus granulosus （E. granulosus） is one of the most important chronic helminthic diseases, especially in sheep/cattle-raising regions. The larval stage of the parasite forms a cyst that grows in the liver, lung, or other organs ofthe host. To ensure a long life in the host tissues, the parasite establishes complex inter-cellular communication systems between its host to allow its differentiation toward each larval stage. Recent studies have reported that this communication is associated with the extracellular signal-regulated kinase （ERK） mitogen-activated protein kinase cascade in helminth parasites, and in particular that these protein kinases might serve as effective targets for a novel chemotherapy for cystic echinococcosis. The aim of the present study investigated the biological function of a novel ERK ortholog from E. granulosus, EgERK. Methods DNA encoding EgERK was isolated from protoscolices of E. granulosus and analyzed using the LA Taq polymerase chain reaction （PCR） approach and bioinformatics. Reverse transcription PCR （RT-PCR） was used to determine the transcription level of the gene at two different larval tissues. Western blotting was used to detect levels of EgERK protein. The expression profile of EgERK in protoscolices was examined by immunofluorescence. Results We cloned the entire Egerk genomic locus from E. granulosus. In addition, two alternatively spliced transcripts of Egerk, Egerk-A, and Egerk-B were identified. Egerk-A was found to constitutively expressed at the transcriptional and protein levels in two different larval tissues （cyst membranes and protoscol（ces）. Egerk-A was expressed in the tegumental structures, hooklets, and suckers and in the tissue surrounding the rostellum of E. granulosus protoscolices. Conclusions We have cloned the genomic DNA of a novel ERK ortholog from E. granulosus, EgERK （GenBank ID HQ585923）, and found that it is constitutively expressed in cyst membrane and protoscolex. These findings will be useful in further study of the biological functions of the gene in the growth and development of Echinococcus and will contribute to research on novel anti-echinococcosis drug targets.展开更多
文摘目的观察针刺配合口服真武汤治疗原发性慢性肾病的临床疗效。方法将90例原发性慢性肾病患者随机分为A组、B组和C组,每组30例。A组采用常规营养及对症治疗,B组在A组基础上采用口服真武汤治疗,C组在A组基础上采用针刺配合口服真武汤治疗。观察3组治疗前后各项生化指标[血清肌酐(SCr)、血尿素氮(BUN)、血尿酸(UA)、24 h尿蛋白定量(24 h UPro)、肾小球滤过率(GFR)水平]及右肾皮质、髓质各项血流量灌注指标[皮质到达时间(AT)、达峰值时间(PT)、峰值强度(PI)、上升支斜率(A)、曲线下面积(AUC)]的变化情况,并比较3组临床疗效。结果B组和C组治疗后各项生化指标及右肾皮质、髓质各项血流量灌注指标(AT、PT、PI、AUC)与同组治疗前比较,差异均具有统计学意义(P<0.05)。A组治疗后SCr、24 h UPro、GFR指标及右肾皮质、髓质PT与同组治疗前比较,差异均具有统计学意义(P<0.05)。B组和C组治疗后BUN指标及右肾皮质、髓质AT、PT与A组比较,差异均具有统计学意义(P<0.05)。A组总有效率为66.7%,B组为86.7%,C组为93.3%。C组总有效率与A组和B组比较,差异均具有统计学意义(P<0.05);B组总有效率与A组比较,差异具有统计学意义(P<0.05)。结论针刺配合口服真武汤是一种治疗原发性慢性肾病的有效方法,能改善患者肾功能及肾血流灌注状态。
基金This work was supported by grants from the National Science Foundation of China （No. 30960341 and No. 30860253）, and Xinjiang Key-Lab Project Grants on Echinococcosis （No. XJDX0202-2009-03）.
文摘Background Cystic echinococcosis due to Echinococcus granulosus （E. granulosus） is one of the most important chronic helminthic diseases, especially in sheep/cattle-raising regions. The larval stage of the parasite forms a cyst that grows in the liver, lung, or other organs ofthe host. To ensure a long life in the host tissues, the parasite establishes complex inter-cellular communication systems between its host to allow its differentiation toward each larval stage. Recent studies have reported that this communication is associated with the extracellular signal-regulated kinase （ERK） mitogen-activated protein kinase cascade in helminth parasites, and in particular that these protein kinases might serve as effective targets for a novel chemotherapy for cystic echinococcosis. The aim of the present study investigated the biological function of a novel ERK ortholog from E. granulosus, EgERK. Methods DNA encoding EgERK was isolated from protoscolices of E. granulosus and analyzed using the LA Taq polymerase chain reaction （PCR） approach and bioinformatics. Reverse transcription PCR （RT-PCR） was used to determine the transcription level of the gene at two different larval tissues. Western blotting was used to detect levels of EgERK protein. The expression profile of EgERK in protoscolices was examined by immunofluorescence. Results We cloned the entire Egerk genomic locus from E. granulosus. In addition, two alternatively spliced transcripts of Egerk, Egerk-A, and Egerk-B were identified. Egerk-A was found to constitutively expressed at the transcriptional and protein levels in two different larval tissues （cyst membranes and protoscol（ces）. Egerk-A was expressed in the tegumental structures, hooklets, and suckers and in the tissue surrounding the rostellum of E. granulosus protoscolices. Conclusions We have cloned the genomic DNA of a novel ERK ortholog from E. granulosus, EgERK （GenBank ID HQ585923）, and found that it is constitutively expressed in cyst membrane and protoscolex. These findings will be useful in further study of the biological functions of the gene in the growth and development of Echinococcus and will contribute to research on novel anti-echinococcosis drug targets.
