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基于DNA条形码技术的珠江口春季鱼卵和仔稚鱼种类组成和分布特征的研究 被引量:4
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作者 孔啸兰 张帅 +3 位作者 陈作志 林昭进 蒋佩文 江艳娥 《海洋学报》 CAS CSCD 北大核心 2023年第3期52-65,共14页
采用线粒体COⅠ和12S rRNA基因片段作为DNA条形码,分析珠江口春季鱼卵和仔稚鱼种类组成和分布特征,并探究两种条形码在鱼卵和仔稚鱼种类鉴定中的适用性。研究共扩增样本391个,成功鉴定的鱼卵和仔稚鱼共7目25科42属60种(2种未鉴定到种)... 采用线粒体COⅠ和12S rRNA基因片段作为DNA条形码,分析珠江口春季鱼卵和仔稚鱼种类组成和分布特征,并探究两种条形码在鱼卵和仔稚鱼种类鉴定中的适用性。研究共扩增样本391个,成功鉴定的鱼卵和仔稚鱼共7目25科42属60种(2种未鉴定到种)。其中,以鲈形目(Perciformes)种类和数量最多,种类数占比为51.6%,数量占比为47.91%;其次为鲱形目(Clupeiformes),种类数占比为25%,数量占比为34.56%。优势种10种,其中凤鲚(Coilia mystus)优势度最高,为0.071;棘头梅童鱼(Collichthys lucidus)最低,为0.014。COⅠ和12S rRNA基因片段扩增结果显示,鱼卵和仔稚鱼12S rRNA基因片段扩增成功率(95.60%)明显高于COⅠ基因(43.22%)。遗传距离和ABGD分析显示,COⅠ基因种内遗传距离为0~0.005(平均0.003),种间遗传距离为0.061~0.376(平均0.253),两者间存在明显的“条形码间隙”,ABGD划分结果与数据库比对结果一致;12S r RNA基因种内遗传距离为0~0.011(平均0.007),种间遗传距离为0.007~0.487(平均0.283),龟(Chelon haematocheila)和前鳞龟(Chelon affinis)种间遗传距离与种内遗传距离不形成“条形码间隙”,ABGD将其划分为同一种。系统发育分析显示,在种的分类阶元,所有物种均能聚为独立分支,得到有效区分。综上,线粒体COⅠ和12S rRNA条形码可有效鉴定珠江口大多数鱼卵和仔稚鱼,但是COⅠ基因扩增成功率较低,12S rRNA基因部分近缘物种存在区分困难的情况,两种基因结合使用更能提高鱼卵和仔稚鱼种类鉴定的成功率和准确性。 展开更多
关键词 COⅠ基因 12S rRNA基因 鱼卵和仔稚鱼 DNA条形码
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南海海域鸢乌贼中型群与微型群渔业生物学比较 被引量:8
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作者 江艳娥 陈作志 +3 位作者 林昭进 邱永松 张鹏 方展强 《水产学报》 CAS CSCD 北大核心 2019年第2期454-466,共13页
根据2012年9—10月采集的780尾鸢乌贼样本,对比研究了中型群和微型群两个种群的渔业生物学特征,为南海海域鸢乌贼渔业资源的管理、保护等提供基础资料。按海洋调查规范测定样本的胴长、体质量、性腺指数和摄食指数等生物学指标,通过磨... 根据2012年9—10月采集的780尾鸢乌贼样本,对比研究了中型群和微型群两个种群的渔业生物学特征,为南海海域鸢乌贼渔业资源的管理、保护等提供基础资料。按海洋调查规范测定样本的胴长、体质量、性腺指数和摄食指数等生物学指标,通过磨片测定耳石生长纹数。结果显示,中型群胴长范围79~266 mm,优势胴长为111~145 mm,平均胴长随纬度的变化不明显;微型群胴长范围56~118 mm,优势胴长为66~95 mm,平均胴长随纬度的升高略呈上升趋势。中型群体质量分布范围17.47~957.20 g,优势体质量为45.01~120.00 g;微型群体质量分布范围5.81~76.56 g,优势体质量为5.01~50.00 g;中型群和微型群平均体质量随纬度的升高均有上升的趋势。中型群和微型群两个群体雌性和雄性样本的胴长和体质量之间的关系均适合幂函数。中型群雌雄比例为0.80∶1,未成熟与成熟个体比例为1.12∶1;微型群雌雄比例为3∶1,未成熟与成熟个体比例为0.83∶1。中型群雌性的性腺指数为1.71,雄性为3.31;微型群雌性的性腺指数为4.07,雄性为4.11。中型群雌性的摄食指数为5.07,雄性为3.54;微型群雌性的摄食指数为1.89,雄性为2.75。中型群耳石日龄范围为30~135 d,微型群耳石日龄范围为44~81 d。根据样本捕捞日期,推测微型群和中型群的产卵高峰分别为7月和8月。南海海域鸢乌贼秋季样本中型群个体规格大于微型群个体,2个群体的性成熟和孵化存在错峰现象,微型群雌性性成熟和产卵略早于中型群的雌性。推断7°、14°和16°N海域是鸢乌贼群体在南海的摄食场。 展开更多
关键词 鸢乌贼 渔业生物学 日龄 孵化 南海
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南海北部陆架水域多齿蛇鲻与花斑蛇鲻的年龄与生长 被引量:5
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作者 江艳娥 许友伟 +7 位作者 范江涛 孙铭帅 杨玉滔 张魁 张俊 黄梓荣 林昭进 陈作志 《中国水产科学》 CAS CSCD 北大核心 2019年第1期82-90,共9页
2014年7月至2015年5月采集南海北部陆架海域的多齿蛇鲻(Sauridatumbil)和花斑蛇鲻(Sauridaundosquamis)样本,测量其叉长、体重、性腺成熟度,并读取其耳石和日龄数据,对这2个种的生物学特征进行比较,发现多齿蛇鲻和花斑蛇鲻叉长、体重雌... 2014年7月至2015年5月采集南海北部陆架海域的多齿蛇鲻(Sauridatumbil)和花斑蛇鲻(Sauridaundosquamis)样本,测量其叉长、体重、性腺成熟度,并读取其耳石和日龄数据,对这2个种的生物学特征进行比较,发现多齿蛇鲻和花斑蛇鲻叉长、体重雌雄间差异显著(P<0.05)。多齿蛇鲻雌性与雄性样本,叉长范围分别为109~310 mm、117~283 mm,体重范围分别为13~358 g、20~297 g,日龄范围分别为76~558 d、73~526 d, von Bertalanffy生长方程的相关参数分别为:L_(∞♀)=278 mm, L_(∞♂)=276 mm; K_♀=1.30, K_♂=1.30; t_(0♀)=-0.07 a, t_(0♂)=-0.08 a。雌性性腺成熟度Ⅲ期样本从350~400 d日龄组开始出现,雌性与雄性样本孵化高峰期在3月、7月、9—10月。花斑蛇鲻雌性与雄性样本,叉长范围分别为105~269 mm、95~264 mm,体重范围分别为11~220 g、8~228 g,日龄范围分别为79~486 d、84~488 d, von Bertalanffy生长方程的相关参数分别为:L_(∞♀)=269 mm, L_(∞♂)=265 mm, K_♀=1.29, K_♂=1.29, t_(0♀)=-0.06 a, t_(0♂)=-0.06 a,雌性性腺成熟度Ⅲ期样本从200~250 d日龄组开始出现,雌性与雄性样本孵化高峰期在6—11月。与历史资料进行对比,发现南海海域多齿蛇鲻与花斑蛇鲻出现个体大小逐渐减小、性成熟提前等适应性特征。结论认为,为了南海多齿蛇鲻与花斑蛇鲻渔业资源的可持续发展,需要降低南海近海的捕捞压力以促进南海渔业资源的发展。 展开更多
关键词 南海北部 多齿蛇鲻 花斑蛇鲻 日轮 生长
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基于DNA条形码的江门近岸海域春季鱼卵的种类鉴定及其形态 被引量:3
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作者 陈静 黄德练 +8 位作者 王雪辉 徐磊 张健 李亚芳 宁加佳 王亮根 刘双双 林昭进 杜飞雁 《南方水产科学》 CAS CSCD 北大核心 2022年第6期10-18,共9页
江门位于珠江口西南部,其近海海域是多种小型鱼类的产卵场和育幼场。以珠江口江门海域春季采集到的鱼卵为研究对象,基于DNA条形码技术分析鉴定了鱼卵种类,描述了鱼卵的形态特征,并初步研究了其形态分类。共采获13种鱼卵,分属于3目10科,... 江门位于珠江口西南部,其近海海域是多种小型鱼类的产卵场和育幼场。以珠江口江门海域春季采集到的鱼卵为研究对象,基于DNA条形码技术分析鉴定了鱼卵种类,描述了鱼卵的形态特征,并初步研究了其形态分类。共采获13种鱼卵,分属于3目10科,其中4种鉴定至种水平,分别为眶棘双边鱼(Ambassis gymnocephalus)、缘边银鲈(Gerres limbatus)、多鳞(Sillago sihama)和斑头舌鳎(Cynoglossus puncticeps);8种鉴定至属水平,分别为沙丁鱼属、小公鱼属、棘鲷属、副叶鲹属、叫姑鱼属、属和舌鳎属;1种鉴定至科水平,为鲾科。结果发现,江门海域大部分鱼卵可通过形状与大小、卵周隙宽窄、油球数量和色素分布等形态特征进行分类;对于胚体发育良好的鱼卵,胚体上色素胞颜色、数量及分布也是种类鉴定的重要依据。然而相近种类难以通过单一发育阶段形态特征进行准确区分,更深入的形态学研究尚待开展。 展开更多
关键词 鱼卵 DNA条形码 物种鉴定 形态特征 江门海域
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Population parameters and dynamic pool models of commercial fishes in the Beibu Gulf, northern South China Sea 被引量:13
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作者 WANG Xuehui QIU Yongsong +3 位作者 DU Feiyan lin zhaojin SUN Dianrong HUANG Shuolin 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2012年第1期105-117,共13页
Length-frequency data of eight commercial fish species in the Beibu Gulf (Golf of Tonkin), northern South China Sea, were collected during 2006-2007. Length-weight relationships and growth and mortality parameters w... Length-frequency data of eight commercial fish species in the Beibu Gulf (Golf of Tonkin), northern South China Sea, were collected during 2006-2007. Length-weight relationships and growth and mortality parameters were analyzed using FiSAT II software. Five species had isometric growth, two species had negative allometric growth, and one species had positive allometric growth. Overall, the exploitation rates of the eight species were lower in 2006 2007 than in 1997-1999: for four species (Saurida tumbil, Saurida undosquamis, Argyrosomus macrocephalus, and Nemipterus virgatus) it was lower in 2006-2007 than in 1997 1999, for two species (Parargyrops edita and Trichiurus haumela) it remained the same, and for the other two species (Trachurus japonicus and Decapterus maruadsi) it was higher in 2006~007 than in 1997-1999. The exploitation rates might have declined because of the decline in fishing intensity caused by high crude oil prices. The optimum exploitation rate, estimated using Beverton-Holt dynamic pool models, indicated that although fishes in the Beibu Gulf could sustain high exploitation rates, the under-size fishes at first capture resulted in low yields. To increase the yield per recruitment, it is more effective to increase the size at first capture than to control fishing effort. 展开更多
关键词 commercial fish population parameters dynamic pool model stock status Beibu Gulf northern South China Sea
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Statolith Morphology of the Sthenoteuthis oualaniensis in the Central South China Sea 被引量:1
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作者 Jiang Yan'e Chen Zuozhi +3 位作者 Zhang Peng lin zhaojin Qiu Yongsong Fang Zhanqiang 《Animal Husbandry and Feed Science》 CAS 2015年第5期284-289,共6页
The morphology, microstructure and demographic parameters of statolith of Sthenoteuthis oualaniensis were studied from 90 individuals captured in the central South China Sea in September and October, 2012. The marphol... The morphology, microstructure and demographic parameters of statolith of Sthenoteuthis oualaniensis were studied from 90 individuals captured in the central South China Sea in September and October, 2012. The marphologic results showed that the statolith has great lateral dome and wing dome, small dorsal dome, and long narrow rostrum dome. S. Oualaniensis could be divided into three populations by statolith morphology in the central South China Sea. Five most important featured parameters including total statolith length (TSL), wing length (WL), maximum width (MW) were described by logarithmic functions with mental length (ML) (P 〈 0.05 ) ; rostnnn length ( RSL), dorsolateral length (DLL) were described by linear functions with ML( P 〈 0.05 ). With the squid growth, the size of statolith gradually develops, while the ratio of each featured parameter to ML slowly decreases, the ratio of DLL, LDL, RSL and WL to TSL almost remain the same level, corresponding to 48.05%, 60.05%, 35.44%, 67.59%, and the ratio of LDL and WL to SL show little higher. 展开更多
关键词 Sthenoteuthis oualaniensis STATOLITH MORPHOLOGY South China Sea
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Seasonal variations in diversity and abundance of surface ichthyoplankton in the northern South China Sea
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作者 LI Kaizhi YIN Jianqiang +1 位作者 HUANG Liangmin lin zhaojin 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2014年第12期145-154,共10页
The objective of this study was to investigate the seasonal variations of ichthyoplankton diversity and abun-dance in the northern South China Sea based on the data collected during summer, winter and spring. In total... The objective of this study was to investigate the seasonal variations of ichthyoplankton diversity and abun-dance in the northern South China Sea based on the data collected during summer, winter and spring. In total, 95 taxa of larval fishes were identified. The greatest number of species was recorded in spring, followed by summer and winter. The number of species was distributed mainly in the coastal waters from the east of Leizhou Peninsula to the southeast of Hainan Island during the surveyed periods of summer and spring, but in the offshore waters during winter. The abundance of larval fish was lowest in winter, increased in spring, and reached the maximum in summer. High abundance of larval fish was generally restricted to coastal waters with the isobaths less than 50 m. Seasonal variations of larval fish richness, abundance and diversity index were significant (P〈0.001). Carangidae was the most common and abundant taxon in summer and winter, whileSardinella sp.,Thrissa mystax andLeiognathus sp. were dominant in spring. High diversity and abundance of larval fish might be attributed to increased temperature and coastal upwelling in spring and summer. 展开更多
关键词 species composition ABUNDANCE ICHTHYOPLANKTON CARANGIDAE northern South China Sea
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