Ogura cytoplasmic male sterility(Ogura CMS)is extensively applied in hybrid seed production in cruciferous crops.However,the posttranscriptional molecular basis of Ogura CMS in cruciferous crops remains elusive.Here,a...Ogura cytoplasmic male sterility(Ogura CMS)is extensively applied in hybrid seed production in cruciferous crops.However,the posttranscriptional molecular basis of Ogura CMS in cruciferous crops remains elusive.Here,a data-independent acquisition-based proteomic approach coupled with a parallel reaction monitoring-based targeted proteomic assay was used to analyze the proteome dynamics of Ogura CMS cabbage line RM and its maintainer line RF during floral bud development to obtain insights into the mechanism underlying Ogura CMS in cruciferous crops.A total of 9162 proteins corresponding to 61464 peptides were identified in RM and RF floral buds.The proteomic fluctuation of RM was weaker than that of RF.Differences in protein expression between RM and RF gradually enlarged with floral bud development.Fifteen continually up-regulated and eight continually down-regulated proteins were found in RM relative to RF throughout floral bud development.Differentially expressed proteins between RM and RF during floral bud development were implicated in the endoplasmic reticulum(ER)-associated protein processing pathway,in which most of them exhibited down-regulated expression in RM.These data suggest that ER-associated protein processing may be involved in pollen abortion in Ogura CMS cabbage by inhibiting the expression of critical factors.Our findings not only deepen the understanding of the molecular mechanisms of Ogura CMS in cruciferous crops but also provide better guidance for applying Ogura CMS in the hybrid breeding of cruciferous crops.展开更多
Background:Nasopharyngeal carcinoma(NPC)exhibits a significant prevalence in the southern regions of China,and paclitaxel(PTX)is frequently employed as a medication for managing advanced NPC.However,drug resistance is...Background:Nasopharyngeal carcinoma(NPC)exhibits a significant prevalence in the southern regions of China,and paclitaxel(PTX)is frequently employed as a medication for managing advanced NPC.However,drug resistance is typically accompanied by a poor prognosis.Exploring the synergistic potential of combining multiple chemotherapeutic agents may represent a promising avenue for optimizing treatment efficacy.Methods:This study investigated whether 3-Methyladenine(3-MA)could potentiated the effect of PTX and its potential molecular mechanism.Samples were divided into the following categories:Negative control(NC)with the solvent dimethyl sulfoxide(DMSO,0.5%v/v),PTX(400 nM),3-MA(4 mM),and PTX(400 nM)+3-MA(4 mM).The viability of NPC cells was assessed using both the cell counting kit-8(CCK-8)assay and the colony formation assay.Microscopic observation was performed to identify morphological cell changes.Flow cytometry was used to assess cell cycle status,mitochondrial membrane potential(MMP),and apoptotic cells.Western blotting was conducted to quantify the protein expression.Results:3-MA enhanced PTX-specific inhibition of NPC cell proliferation.PTX,either alone or in combination with 3-MA,caused cell cycle halt at the G2/M phase in the majority of NPC cells,and the combination treatment of PTX with 3-MA induced a higher rate of NPC cell death compared to PTX alone.Western blotting results revealed the combination of PTX with 3-MA heightened activation of cyclin-dependent kinase 1(CDK1),a key molecule in shifting cells from mitotic arrest to apoptosis,led to a reduction in Myeloid Cell Leukemia 1(MCL-1)expression and an increase in Poly(ADP-ribose)polymerase(PARP)cleavage.Conclusion:The concurrent administration of PTX with 3-MA effectively enhances PTX’s inhibitory impact on NPC and activates the apoptosis signal regulated by CDK1.展开更多
Background The alexithymia trait is of high clinical interest.The Perth Alexithymia Questionnaire(PAQ)was recently developed to enable detailed facet-level and valence-specific assessments of alexithymia.Aims In this ...Background The alexithymia trait is of high clinical interest.The Perth Alexithymia Questionnaire(PAQ)was recently developed to enable detailed facet-level and valence-specific assessments of alexithymia.Aims In this paper,we introduce the first Chinese version of the PAQ and examine its psychometric properties and clinical applications.Methods In Study 1,the PAQ was administered to 990 Chinese participants.We examined its factor structure,internal consistency,test-retest reliability,as well as convergent,concurrent and discriminant validity.In Study 2,four groups,including a major depressive disorder(MDD)group(n=50),a matched healthy control group for MDD(n=50),a subclinical depression group(n=50)and a matched healthy control group for subclinical depression(n=50),were recruited.Group comparisons were conducted to assess the clinical relevance of the PAQ.Results In Study 1,the intended five-factor structure of the PAQ was found to fit the data well.The PAQ showed good internal consistency and test-retest reliability,as well as good convergent,concurrent and discriminant validity.In Study 2,the PAQ was able to successfully distinguish the MDD group and the subclinical depression group from their matched healthy controls.Conclusions The Chinese version of the PAQ is a valid and reliable instrument for comprehensively assessing alexithymia in the general population and adults with clinical/subclinical depression.展开更多
<div style="text-align:justify;"> Precipitation is an important part of the global hydrological cycle. The large- scale, high-precision continuous precipitation data obtained by satellite remote sensin...<div style="text-align:justify;"> Precipitation is an important part of the global hydrological cycle. The large- scale, high-precision continuous precipitation data obtained by satellite remote sensing detection technology has become an important source of spatial precipitation data. However, because the spatial resolution of remote sensing precipitation data is still low, it is difficult to meet the needs of hydrological research, which restricts their application in drought and flood analysis, hydrological simulation, etc. In response to this problem, this paper takes the Beijing-Tianjin-Hebei region as the research area, downscaling the TRMM data and the GPM data space of the continuation plan, and increasing the spatial resolution of the data to 1 km. Compared with the original data, spatial downscaling data not only greatly improves the spatial resolution, but also increases the accuracy of the data, which has better applicability. </div>展开更多
Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and disability.Accurate predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic ...Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and disability.Accurate predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic stroke remain undetermined.With innovations in high-throughput gene sequencing analysis,many aberrantly expressed non-coding RNAs(ncRNAs)in the brain and peripheral blood after acute ischemic stroke have been found in clinical samples and experimental models.Differentially expressed ncRNAs in the post-stroke brain were demonstrated to play vital roles in pathological processes,leading to neuroprotection or deterioration,thus ncRNAs can serve as therapeutic targets in acute ischemic stroke.Moreover,distinctly expressed ncRNAs in the peripheral blood can be used as biomarkers for acute ischemic stroke prediction,diagnosis,and prognosis.In particular,ncRNAs in peripheral immune cells were recently shown to be involved in the peripheral and brain immune response after acute ischemic stroke.In this review,we consolidate the latest progress of research into the roles of ncRNAs(microRNAs,long ncRNAs,and circular RNAs)in the pathological processes of acute ischemic stroke–induced brain damage,as well as the potential of these ncRNAs to act as biomarkers for acute ischemic stroke prediction,diagnosis,and prognosis.Findings from this review will provide novel ideas for the clinical application of ncRNAs in acute ischemic stroke.展开更多
Objective Renal fibrosis is the ultimate pathway of various forms of acute and chronic kidney damage.Notably,the knockout of transient receptor potential channel 6(TRPC6)has shown promise in alleviating renal fibrosis...Objective Renal fibrosis is the ultimate pathway of various forms of acute and chronic kidney damage.Notably,the knockout of transient receptor potential channel 6(TRPC6)has shown promise in alleviating renal fibrosis.However,the regulatory impact of TRPC6 on renal fibrosis remains unclear.Methods In vivo,TRPC6 knockout(TRPC6−/−)mice and age-matched 129 SvEv(WT)mice underwent unilateral renal ischemia-reperfusion(uIR)injury surgery on the left renal pedicle or sham operation.Kidneys and serum were collected on days 7,14,21,and 28 after euthanasia.In vitro,primary tubular epithelial cells(PTECs)were isolated from TRPC6−/−and WT mice,followed by treatment with transforming growth factorβ1(TGFβ1)for 72 h.The anti-fibrotic effect of TRPC6−/−and the underlying mechanisms were assessed through hematoxylin-eosin staining,Masson staining,immunostaining,qRT-PCR,and Western blotting.Results Increased TRPC6 expression was observed in uIR mice and PTECs treated with TGFβ1.TRPC6−/−alleviated renal fibrosis by reducing the expression of fibrotic markers(Col-1,α-SMA,and vimentin),as well as decreasing the apoptosis and inflammation of PTECs during fibrotic progression both in vivo and in vitro.Additionally,we found that the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/glycogen synthase kinase 3 beta(GSK3β)signaling pathway,a pivotal player in renal fibrosis,was down-regulated following TRPC6 deletion.Conclusion These results suggest that the ablation of TRPC6 may mitigate renal fibrosis by inhibiting the apoptosis and inflammation of PTECs through down-regulation of the PI3K/AKT/GSK3βpathway.Targeting TRPC6 could be a novel therapeutic strategy for preventing chronic kidney disease.展开更多
目的:观察穴位按压对老年转移性结直肠癌维持治疗患者癌因性疲乏(cancer related fatigue,CRF)的干预效果。方法:前瞻性收集98例存在CRF的老年(≥65岁)转移性结直肠癌维持治疗患者,参照随机数字表法随机分为观察组50例和对照组48例。对...目的:观察穴位按压对老年转移性结直肠癌维持治疗患者癌因性疲乏(cancer related fatigue,CRF)的干预效果。方法:前瞻性收集98例存在CRF的老年(≥65岁)转移性结直肠癌维持治疗患者,参照随机数字表法随机分为观察组50例和对照组48例。对照组仅接受常规健康指导,观察组在此基础上给予穴位按压,双侧取穴合谷、三阴交、足三里,按压手法经研究者培训指导后,由患者自行操作,每次每侧每个穴位持续按压5 min,共30 min,每天3次。两组均连续干预6周。干预前后,采用Piper疲乏修订量表和匹兹堡睡眠质量指数量表评估患者疲乏和睡眠质量变化情况,检测外周血T淋巴细胞亚群指标。结果:干预后,观察组Piper疲乏修订量表和匹兹堡睡眠质量指数量表评分低于对照组,观察组外周血CD3^(+)、CD3^(+)CD4^(+)和CD4^(+)/CD8^(+)水平高于对照组,差异有统计学意义(P<0.05)。结论:穴位按压有助于减轻老年转移性结直肠癌维持治疗患者CRF,提高睡眠质量,可能与改善患者自身免疫功能有关。展开更多
Xuefu Zhuyu decoction has been used for treating traumatic brain injury and improving post-traumatic dysfunction, but its mechanism of action needs further investigation. This study established rat models of traumatic...Xuefu Zhuyu decoction has been used for treating traumatic brain injury and improving post-traumatic dysfunction, but its mechanism of action needs further investigation. This study established rat models of traumatic brain injury by controlled cortical impact. Rat models were intragastrically administered 9 and 18 g/kg Xuefu Zhuyu decoction once a day for 14 or 21 days. Changes in neurological function were assessed by modified neurological severity scores and the Morris water maze. Immunohistochemistry, western blot assay, and re- verse-transcription polymerase chain reaction were used to analyze synapsin protein and mRNA expression at the injury site of rats. Our results showed that Xuefu Zhuyu decoction visibly improved neurological function of rats with traumatic brain injury. These changes were accompanied by increased expression of synaptophysin, synapsin I, and postsynaptic density protein-95 protein and mRNA in a dose-de- pendent manner. These findings indicate that Xuefu Zhuyu decoction increases synapsin expression and improves neurological deficits alder traumatic brain injury.展开更多
Objective: Recurrent ovarian cancer is often resistant to drugs such as paclitaxel. Short hairpin RNA (shRNA) targeting MDRI, a gene involved in the process of drug resistance, may be a promising strategy to overco...Objective: Recurrent ovarian cancer is often resistant to drugs such as paclitaxel. Short hairpin RNA (shRNA) targeting MDRI, a gene involved in the process of drug resistance, may be a promising strategy to overcome drug resistance. Methods: Construction and identification of eukaryotic expression plasmid was transiently transfected into human ovarian cancer ce plasmid of shRNA targeting on MDR1 gene. The ne A2780/Taxol. Apoptosis was determined by flow cytometry using annexin V-FITC/PI double labeling. Expression of MDRI mRNA was detected by quantitative polymerase chain reaction (qPCR) and P-glycoprotein expression was detected using Western blot. Results The IC50 of paclitaxel in MDR1 shRNA-transfected group was significantly reduced (1.986±0.153)μmol/ml as compared with that in negative control (5.246±0.107)μmol/ml and empty vector-transfected group (5.212±0.075)μmol/ml (P〈0.05). The percent of the relative reverse sensitivity to paclitaxel on A2780/Taxol cells was 67.1%, and the apoptotic rate was significantly increased [(6.977±0.333)%] compared with control [(1.637±0.111)%] and empty vector-transfected group [(1.663±0.114)%] (P〈0.05). Expressions of MDR1 mRNA and P-glycoprotein were significantly reduced compared with control (P〈0.05). Conclusion: The present study demonstrated that the eukaryotic expression plasmid of shRNA targeting on MDRI inhibited the expression of MDRI effectively, thus enhance the sensitivity of A2780/Taxol cells to paclitaxel.展开更多
Objective To investigate reliability of the infra-nipple broken line incision for breast augmentation. Methods From January 2012 to January 2013, 15 patients underwent primary bilateral retromuscular breast augmentati...Objective To investigate reliability of the infra-nipple broken line incision for breast augmentation. Methods From January 2012 to January 2013, 15 patients underwent primary bilateral retromuscular breast augmentation with round textured silicone-gel implants and a novel infra-nipple broken line incision. Preoperatively, a semicircular incision was marked along the inferior base of the nipple. It was then extended bilaterally using two transverse right-angled geometric broken lines within the pigmented areolar skin. Follow-up was performed to evaluate the sensation of nipple-areolar complex, the scar, and the shape and texture of the breasts. Results The average follow-up was 6.7 months. Most of the patients complained of paresthesia of the nipple or breast skin, but transient decreased sensation improved within 3 months. No patients showed permanent sensory changes of the nipple areolar complex at a minimum follow-up of 4 months. The scars were imperceptible in all patients. Conclusion We believe that for selected patients, the infra-nipple broken line incision is a practical and reliable method to achieve aesthetic result.展开更多
In drilling a deepwater well,the mud density window is narrow,which needs a precise pressure control to drill the well to its designed depth.Therefore,an accurate characterization of annular flow between the drilling ...In drilling a deepwater well,the mud density window is narrow,which needs a precise pressure control to drill the well to its designed depth.Therefore,an accurate characterization of annular flow between the drilling riser and drilling string is critical in well control and drilling safety.Many other factors influencing the change of drilling pressure that should be but have not been studied sufficiently.We used numerical method to simulate the process of drill string rotation and vibration in the riser to show that the rotation and transverse vibration of drill string can increase the axial velocity in the annulus,which results in the improvement of the flow field in the annulus,and the effect on pressure loss and its fluctuation amplitude.In addition,there are also multiple secondary flow vortices in the riser annulus under certain eccentricity conditions,which is different from the phenomenon in an ordinary wellbore.The findings of this research are critical in safely controlling well drilling operation in the deepwater environment.展开更多
Many studies have investigated how the online community has informed language acquisition and teaching.In the scholarship of Computer-Mediated Communication Theory(CMC),while most of the studies have emphasized second...Many studies have investigated how the online community has informed language acquisition and teaching.In the scholarship of Computer-Mediated Communication Theory(CMC),while most of the studies have emphasized second language English writing using the CMC framework,evidence is shown to support CMC in foreign language instruction.However,there is a lack of research on how feedback from native speakers of Chinese influences Chinese learners'writing in a web environment.In response to the research gap,drawing on sociocultural and CMC,the current exploratory study probes the nature of feedback on Zhihu,the largest Chinese social question-and-answer website,and how feedback affects advanced Chinese learners'writing performance.Eight American university students learning Chinese as second language participated by completing two writing tasks on Zhihu.After posting essays on the website,these participants read and responded to online feedback within two weeks,followed by a revision of their original posts.Tokens of feedback,revised language items and learners'response to feedback were coded by the researcher.Findings reported feedback types from Zhihu users,exchanges between learners and feedback providers,and correlations between first and second drafts.Feedback on Zhihu is proven to be beneficial to advanced Chinese learners'writing performance,and pedagogical implication is suggested for the inclusion of Zhihu in advanced Chinese curriculum.展开更多
Qingke(highland barley)Baijiu is a special Chinese Baijiu which is mainly produced from Qinghai-Tibet Plateau.Since the pine board is used as the bottom of the fermentation pit,we deduced that the terpenoids and noris...Qingke(highland barley)Baijiu is a special Chinese Baijiu which is mainly produced from Qinghai-Tibet Plateau.Since the pine board is used as the bottom of the fermentation pit,we deduced that the terpenoids and norisoprenoids in pine board might be introduced into Qingke Baijiu.Thus,the terpenoids and norisoprenoids in Qingke Baijiu were investigated by headspace solid phase microextraction(HS-SPME)combined with comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry(GC×GC-TOFMS).The results showed thatγ-terpinene(0.70-530.72μg/L),α-phellandrene(0.34-256.66μg/L),longicyclene(0-38.55μg/L),α-pinene(1.21-35.54μg/L)and limonene(0.93-23.69μg/L)were the top 5 terpenoids/norisoprenoids in Qingke Baijiu.Pulegone was detected and reported for the first time in Baijiu,and the concentrations in Qingke Baijiu were 0.78μg/L(fresh)and 3.90μg/L(7 years old).According to the principal component analysis(PCA)plot,young,aged,and retail Qingke Baijiu could be differentiated clearly.Fold change(FC)and t-tests analysis indicated thatβ-pinene,γ-terpinene,andα-selinene were the most different terpenoids/norisoprenoids between young and aged Qingke Baijiu,and longifolene was the most different terpenoids/norisoprenoids between base and retail Qingke Baijiu.The terpenoids and norisoprenoids,such as(E)-β-ionone,isoborneol andβ-cyclocitral,could be potential markers indicating the ageing process of Qingke Baijiu.展开更多
Cyclophilin(CYP)plays an important role in plant response to stress,and OsCYP2,one gene of cyclophlilin family,is involved in auxin signal transduction and stress signaling in rice.However,the mechanism that OsCYP2 is...Cyclophilin(CYP)plays an important role in plant response to stress,and OsCYP2,one gene of cyclophlilin family,is involved in auxin signal transduction and stress signaling in rice.However,the mechanism that OsCYP2 is involved in rice response to low temperature is still unclear.We identified a new OsCYP2 allelic mutant,lrl3,with fewer lateral roots,and the differences in shoot height,primary root length and adventitious root length increased with the growth process compared to the wild-type plant.Auxin signaling pathway was also affected and became insensitive to gravity.The transgenic rice plants with over-expression of OsCYP2 were more tolerant to low temperature than the wild-type plants,suggesting that OsCYP2 was involved in the low temperature response in rice.In addition,OsCYP2 negatively regulated the expression of OsTPS38,a terpene synthase gene,and was dependent on the OsCDPK7-mediated pathway in response to low temperature stress.OsTPS38-overexpressed transgenic line ox-2 was more sensitive to low temperature.Therefore,OsCYP2 may negatively regulate OsTPS38 through an OsCDPK7-dependent pathway to mediate the response to low temperature in rice.These results provide a new basis for auxin signaling genes to regulate rice response to low temperature stress.展开更多
基金supported by Heilongjiang Provincial Natural Science Foundation of China(Grant No.YQ2022C012)China Postdoctoral Science Foundation(Grant No.2022MD713728)+1 种基金Heilongjiang Provincial Postdoctoral Fund(Grant No.LBHZ21046)the Open Project of Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Ministry of Agriculture and Rural Affairs,and National Key Research and Development Program of China(Grant No.2023YFD1201501).
文摘Ogura cytoplasmic male sterility(Ogura CMS)is extensively applied in hybrid seed production in cruciferous crops.However,the posttranscriptional molecular basis of Ogura CMS in cruciferous crops remains elusive.Here,a data-independent acquisition-based proteomic approach coupled with a parallel reaction monitoring-based targeted proteomic assay was used to analyze the proteome dynamics of Ogura CMS cabbage line RM and its maintainer line RF during floral bud development to obtain insights into the mechanism underlying Ogura CMS in cruciferous crops.A total of 9162 proteins corresponding to 61464 peptides were identified in RM and RF floral buds.The proteomic fluctuation of RM was weaker than that of RF.Differences in protein expression between RM and RF gradually enlarged with floral bud development.Fifteen continually up-regulated and eight continually down-regulated proteins were found in RM relative to RF throughout floral bud development.Differentially expressed proteins between RM and RF during floral bud development were implicated in the endoplasmic reticulum(ER)-associated protein processing pathway,in which most of them exhibited down-regulated expression in RM.These data suggest that ER-associated protein processing may be involved in pollen abortion in Ogura CMS cabbage by inhibiting the expression of critical factors.Our findings not only deepen the understanding of the molecular mechanisms of Ogura CMS in cruciferous crops but also provide better guidance for applying Ogura CMS in the hybrid breeding of cruciferous crops.
基金supported by the Science and Technology Innovation Program of Hunan Province(Grant Numbers:2021SK1014 and 2022WZ1027)the Colleges and Universities of Hunan Province(Grant Number:HNJG 20200440)+1 种基金the Scientific Research Fund of Hunan Provincial Education Department(Grant Number:21B0411)the Scientific Research Project of Changsha Central Hospital(Number:YNKY202201).
文摘Background:Nasopharyngeal carcinoma(NPC)exhibits a significant prevalence in the southern regions of China,and paclitaxel(PTX)is frequently employed as a medication for managing advanced NPC.However,drug resistance is typically accompanied by a poor prognosis.Exploring the synergistic potential of combining multiple chemotherapeutic agents may represent a promising avenue for optimizing treatment efficacy.Methods:This study investigated whether 3-Methyladenine(3-MA)could potentiated the effect of PTX and its potential molecular mechanism.Samples were divided into the following categories:Negative control(NC)with the solvent dimethyl sulfoxide(DMSO,0.5%v/v),PTX(400 nM),3-MA(4 mM),and PTX(400 nM)+3-MA(4 mM).The viability of NPC cells was assessed using both the cell counting kit-8(CCK-8)assay and the colony formation assay.Microscopic observation was performed to identify morphological cell changes.Flow cytometry was used to assess cell cycle status,mitochondrial membrane potential(MMP),and apoptotic cells.Western blotting was conducted to quantify the protein expression.Results:3-MA enhanced PTX-specific inhibition of NPC cell proliferation.PTX,either alone or in combination with 3-MA,caused cell cycle halt at the G2/M phase in the majority of NPC cells,and the combination treatment of PTX with 3-MA induced a higher rate of NPC cell death compared to PTX alone.Western blotting results revealed the combination of PTX with 3-MA heightened activation of cyclin-dependent kinase 1(CDK1),a key molecule in shifting cells from mitotic arrest to apoptosis,led to a reduction in Myeloid Cell Leukemia 1(MCL-1)expression and an increase in Poly(ADP-ribose)polymerase(PARP)cleavage.Conclusion:The concurrent administration of PTX with 3-MA effectively enhances PTX’s inhibitory impact on NPC and activates the apoptosis signal regulated by CDK1.
基金This work was supported by a grant from the National Natural Science Foundation of China(32200906)STI 2030-Major Projects(2021ZD0201705)+2 种基金Major Project of Philosophy and Social Science Research of the Ministry of Education of China(22JZD044)Research Project of Shanghai Science and Technology Commission(20dz2260300)Fundamental Research Funds for the Central Universities and Starting Research Fund from Hangzhou Normal University.
文摘Background The alexithymia trait is of high clinical interest.The Perth Alexithymia Questionnaire(PAQ)was recently developed to enable detailed facet-level and valence-specific assessments of alexithymia.Aims In this paper,we introduce the first Chinese version of the PAQ and examine its psychometric properties and clinical applications.Methods In Study 1,the PAQ was administered to 990 Chinese participants.We examined its factor structure,internal consistency,test-retest reliability,as well as convergent,concurrent and discriminant validity.In Study 2,four groups,including a major depressive disorder(MDD)group(n=50),a matched healthy control group for MDD(n=50),a subclinical depression group(n=50)and a matched healthy control group for subclinical depression(n=50),were recruited.Group comparisons were conducted to assess the clinical relevance of the PAQ.Results In Study 1,the intended five-factor structure of the PAQ was found to fit the data well.The PAQ showed good internal consistency and test-retest reliability,as well as good convergent,concurrent and discriminant validity.In Study 2,the PAQ was able to successfully distinguish the MDD group and the subclinical depression group from their matched healthy controls.Conclusions The Chinese version of the PAQ is a valid and reliable instrument for comprehensively assessing alexithymia in the general population and adults with clinical/subclinical depression.
文摘<div style="text-align:justify;"> Precipitation is an important part of the global hydrological cycle. The large- scale, high-precision continuous precipitation data obtained by satellite remote sensing detection technology has become an important source of spatial precipitation data. However, because the spatial resolution of remote sensing precipitation data is still low, it is difficult to meet the needs of hydrological research, which restricts their application in drought and flood analysis, hydrological simulation, etc. In response to this problem, this paper takes the Beijing-Tianjin-Hebei region as the research area, downscaling the TRMM data and the GPM data space of the continuation plan, and increasing the spatial resolution of the data to 1 km. Compared with the original data, spatial downscaling data not only greatly improves the spatial resolution, but also increases the accuracy of the data, which has better applicability. </div>
基金supported by the National Natural Science Foundation of China,Nos.82301486(to SL)and 82071325(to FY)Medjaden Academy&Research Foundation for Young Scientists,No.MJR202310040(to SL)+2 种基金Nanjing Medical University Science and Technique Development,No.NMUB20220060(to SL)Medical Scientific Research Project of Jiangsu Commission of Health,No.ZDA2020019(to JZ)Health China Buchang Zhiyuan Public Welfare Project for Heart and Brain Health,No.HIGHER202102(to QD).
文摘Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and disability.Accurate predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic stroke remain undetermined.With innovations in high-throughput gene sequencing analysis,many aberrantly expressed non-coding RNAs(ncRNAs)in the brain and peripheral blood after acute ischemic stroke have been found in clinical samples and experimental models.Differentially expressed ncRNAs in the post-stroke brain were demonstrated to play vital roles in pathological processes,leading to neuroprotection or deterioration,thus ncRNAs can serve as therapeutic targets in acute ischemic stroke.Moreover,distinctly expressed ncRNAs in the peripheral blood can be used as biomarkers for acute ischemic stroke prediction,diagnosis,and prognosis.In particular,ncRNAs in peripheral immune cells were recently shown to be involved in the peripheral and brain immune response after acute ischemic stroke.In this review,we consolidate the latest progress of research into the roles of ncRNAs(microRNAs,long ncRNAs,and circular RNAs)in the pathological processes of acute ischemic stroke–induced brain damage,as well as the potential of these ncRNAs to act as biomarkers for acute ischemic stroke prediction,diagnosis,and prognosis.Findings from this review will provide novel ideas for the clinical application of ncRNAs in acute ischemic stroke.
基金supported by grants from the National Natural Science Foundation of China(Nos.31171087,30970662,and 82100715)the Henan Medical Science and Technology Research Program(Joint Construction,No.LHGJ20190246).
文摘Objective Renal fibrosis is the ultimate pathway of various forms of acute and chronic kidney damage.Notably,the knockout of transient receptor potential channel 6(TRPC6)has shown promise in alleviating renal fibrosis.However,the regulatory impact of TRPC6 on renal fibrosis remains unclear.Methods In vivo,TRPC6 knockout(TRPC6−/−)mice and age-matched 129 SvEv(WT)mice underwent unilateral renal ischemia-reperfusion(uIR)injury surgery on the left renal pedicle or sham operation.Kidneys and serum were collected on days 7,14,21,and 28 after euthanasia.In vitro,primary tubular epithelial cells(PTECs)were isolated from TRPC6−/−and WT mice,followed by treatment with transforming growth factorβ1(TGFβ1)for 72 h.The anti-fibrotic effect of TRPC6−/−and the underlying mechanisms were assessed through hematoxylin-eosin staining,Masson staining,immunostaining,qRT-PCR,and Western blotting.Results Increased TRPC6 expression was observed in uIR mice and PTECs treated with TGFβ1.TRPC6−/−alleviated renal fibrosis by reducing the expression of fibrotic markers(Col-1,α-SMA,and vimentin),as well as decreasing the apoptosis and inflammation of PTECs during fibrotic progression both in vivo and in vitro.Additionally,we found that the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/glycogen synthase kinase 3 beta(GSK3β)signaling pathway,a pivotal player in renal fibrosis,was down-regulated following TRPC6 deletion.Conclusion These results suggest that the ablation of TRPC6 may mitigate renal fibrosis by inhibiting the apoptosis and inflammation of PTECs through down-regulation of the PI3K/AKT/GSK3βpathway.Targeting TRPC6 could be a novel therapeutic strategy for preventing chronic kidney disease.
文摘目的:观察穴位按压对老年转移性结直肠癌维持治疗患者癌因性疲乏(cancer related fatigue,CRF)的干预效果。方法:前瞻性收集98例存在CRF的老年(≥65岁)转移性结直肠癌维持治疗患者,参照随机数字表法随机分为观察组50例和对照组48例。对照组仅接受常规健康指导,观察组在此基础上给予穴位按压,双侧取穴合谷、三阴交、足三里,按压手法经研究者培训指导后,由患者自行操作,每次每侧每个穴位持续按压5 min,共30 min,每天3次。两组均连续干预6周。干预前后,采用Piper疲乏修订量表和匹兹堡睡眠质量指数量表评估患者疲乏和睡眠质量变化情况,检测外周血T淋巴细胞亚群指标。结果:干预后,观察组Piper疲乏修订量表和匹兹堡睡眠质量指数量表评分低于对照组,观察组外周血CD3^(+)、CD3^(+)CD4^(+)和CD4^(+)/CD8^(+)水平高于对照组,差异有统计学意义(P<0.05)。结论:穴位按压有助于减轻老年转移性结直肠癌维持治疗患者CRF,提高睡眠质量,可能与改善患者自身免疫功能有关。
基金supported by the National Natural Science Foundation of China,No.81673719,81173175 and 81303074a grant from China Postdoctoral Science Foundation,No.2016M600639 and 2017T100614
文摘Xuefu Zhuyu decoction has been used for treating traumatic brain injury and improving post-traumatic dysfunction, but its mechanism of action needs further investigation. This study established rat models of traumatic brain injury by controlled cortical impact. Rat models were intragastrically administered 9 and 18 g/kg Xuefu Zhuyu decoction once a day for 14 or 21 days. Changes in neurological function were assessed by modified neurological severity scores and the Morris water maze. Immunohistochemistry, western blot assay, and re- verse-transcription polymerase chain reaction were used to analyze synapsin protein and mRNA expression at the injury site of rats. Our results showed that Xuefu Zhuyu decoction visibly improved neurological function of rats with traumatic brain injury. These changes were accompanied by increased expression of synaptophysin, synapsin I, and postsynaptic density protein-95 protein and mRNA in a dose-de- pendent manner. These findings indicate that Xuefu Zhuyu decoction increases synapsin expression and improves neurological deficits alder traumatic brain injury.
基金support from laboratory of the second hospital and medical college of Shandong university
文摘Objective: Recurrent ovarian cancer is often resistant to drugs such as paclitaxel. Short hairpin RNA (shRNA) targeting MDRI, a gene involved in the process of drug resistance, may be a promising strategy to overcome drug resistance. Methods: Construction and identification of eukaryotic expression plasmid was transiently transfected into human ovarian cancer ce plasmid of shRNA targeting on MDR1 gene. The ne A2780/Taxol. Apoptosis was determined by flow cytometry using annexin V-FITC/PI double labeling. Expression of MDRI mRNA was detected by quantitative polymerase chain reaction (qPCR) and P-glycoprotein expression was detected using Western blot. Results The IC50 of paclitaxel in MDR1 shRNA-transfected group was significantly reduced (1.986±0.153)μmol/ml as compared with that in negative control (5.246±0.107)μmol/ml and empty vector-transfected group (5.212±0.075)μmol/ml (P〈0.05). The percent of the relative reverse sensitivity to paclitaxel on A2780/Taxol cells was 67.1%, and the apoptotic rate was significantly increased [(6.977±0.333)%] compared with control [(1.637±0.111)%] and empty vector-transfected group [(1.663±0.114)%] (P〈0.05). Expressions of MDR1 mRNA and P-glycoprotein were significantly reduced compared with control (P〈0.05). Conclusion: The present study demonstrated that the eukaryotic expression plasmid of shRNA targeting on MDRI inhibited the expression of MDRI effectively, thus enhance the sensitivity of A2780/Taxol cells to paclitaxel.
文摘Objective To investigate reliability of the infra-nipple broken line incision for breast augmentation. Methods From January 2012 to January 2013, 15 patients underwent primary bilateral retromuscular breast augmentation with round textured silicone-gel implants and a novel infra-nipple broken line incision. Preoperatively, a semicircular incision was marked along the inferior base of the nipple. It was then extended bilaterally using two transverse right-angled geometric broken lines within the pigmented areolar skin. Follow-up was performed to evaluate the sensation of nipple-areolar complex, the scar, and the shape and texture of the breasts. Results The average follow-up was 6.7 months. Most of the patients complained of paresthesia of the nipple or breast skin, but transient decreased sensation improved within 3 months. No patients showed permanent sensory changes of the nipple areolar complex at a minimum follow-up of 4 months. The scars were imperceptible in all patients. Conclusion We believe that for selected patients, the infra-nipple broken line incision is a practical and reliable method to achieve aesthetic result.
基金The research work in this paper is supported by the National Natural Science Foundation of China(Grant No.U1762211)National Key Technologies R&D Program of China(Grant No.2016ZX05022-005),This research is also partially supported by Tubular Goods Research Institute of CNPC and State Key Laboratory of Performance and Structural Safety for Petroleum Tubular Goods and Equipment Material of China National Petroleum Corporation.
文摘In drilling a deepwater well,the mud density window is narrow,which needs a precise pressure control to drill the well to its designed depth.Therefore,an accurate characterization of annular flow between the drilling riser and drilling string is critical in well control and drilling safety.Many other factors influencing the change of drilling pressure that should be but have not been studied sufficiently.We used numerical method to simulate the process of drill string rotation and vibration in the riser to show that the rotation and transverse vibration of drill string can increase the axial velocity in the annulus,which results in the improvement of the flow field in the annulus,and the effect on pressure loss and its fluctuation amplitude.In addition,there are also multiple secondary flow vortices in the riser annulus under certain eccentricity conditions,which is different from the phenomenon in an ordinary wellbore.The findings of this research are critical in safely controlling well drilling operation in the deepwater environment.
文摘Many studies have investigated how the online community has informed language acquisition and teaching.In the scholarship of Computer-Mediated Communication Theory(CMC),while most of the studies have emphasized second language English writing using the CMC framework,evidence is shown to support CMC in foreign language instruction.However,there is a lack of research on how feedback from native speakers of Chinese influences Chinese learners'writing in a web environment.In response to the research gap,drawing on sociocultural and CMC,the current exploratory study probes the nature of feedback on Zhihu,the largest Chinese social question-and-answer website,and how feedback affects advanced Chinese learners'writing performance.Eight American university students learning Chinese as second language participated by completing two writing tasks on Zhihu.After posting essays on the website,these participants read and responded to online feedback within two weeks,followed by a revision of their original posts.Tokens of feedback,revised language items and learners'response to feedback were coded by the researcher.Findings reported feedback types from Zhihu users,exchanges between learners and feedback providers,and correlations between first and second drafts.Feedback on Zhihu is proven to be beneficial to advanced Chinese learners'writing performance,and pedagogical implication is suggested for the inclusion of Zhihu in advanced Chinese curriculum.
基金financially supported by the National Key R&D Program of China (2016YFD0400500)
文摘Qingke(highland barley)Baijiu is a special Chinese Baijiu which is mainly produced from Qinghai-Tibet Plateau.Since the pine board is used as the bottom of the fermentation pit,we deduced that the terpenoids and norisoprenoids in pine board might be introduced into Qingke Baijiu.Thus,the terpenoids and norisoprenoids in Qingke Baijiu were investigated by headspace solid phase microextraction(HS-SPME)combined with comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry(GC×GC-TOFMS).The results showed thatγ-terpinene(0.70-530.72μg/L),α-phellandrene(0.34-256.66μg/L),longicyclene(0-38.55μg/L),α-pinene(1.21-35.54μg/L)and limonene(0.93-23.69μg/L)were the top 5 terpenoids/norisoprenoids in Qingke Baijiu.Pulegone was detected and reported for the first time in Baijiu,and the concentrations in Qingke Baijiu were 0.78μg/L(fresh)and 3.90μg/L(7 years old).According to the principal component analysis(PCA)plot,young,aged,and retail Qingke Baijiu could be differentiated clearly.Fold change(FC)and t-tests analysis indicated thatβ-pinene,γ-terpinene,andα-selinene were the most different terpenoids/norisoprenoids between young and aged Qingke Baijiu,and longifolene was the most different terpenoids/norisoprenoids between base and retail Qingke Baijiu.The terpenoids and norisoprenoids,such as(E)-β-ionone,isoborneol andβ-cyclocitral,could be potential markers indicating the ageing process of Qingke Baijiu.
基金The datasets presented in this study can be found in online repositories.The names of the repository/repositories and accession number(s)can be found below:NCBI-SRA database under the BioProject no.PRJNA732107 and accession nos.SRR14629497,SRR14629496,SRR14629495,and SRR14629494 for the RNA-seq data.
文摘Cyclophilin(CYP)plays an important role in plant response to stress,and OsCYP2,one gene of cyclophlilin family,is involved in auxin signal transduction and stress signaling in rice.However,the mechanism that OsCYP2 is involved in rice response to low temperature is still unclear.We identified a new OsCYP2 allelic mutant,lrl3,with fewer lateral roots,and the differences in shoot height,primary root length and adventitious root length increased with the growth process compared to the wild-type plant.Auxin signaling pathway was also affected and became insensitive to gravity.The transgenic rice plants with over-expression of OsCYP2 were more tolerant to low temperature than the wild-type plants,suggesting that OsCYP2 was involved in the low temperature response in rice.In addition,OsCYP2 negatively regulated the expression of OsTPS38,a terpene synthase gene,and was dependent on the OsCDPK7-mediated pathway in response to low temperature stress.OsTPS38-overexpressed transgenic line ox-2 was more sensitive to low temperature.Therefore,OsCYP2 may negatively regulate OsTPS38 through an OsCDPK7-dependent pathway to mediate the response to low temperature in rice.These results provide a new basis for auxin signaling genes to regulate rice response to low temperature stress.
基金supported by projects of the National Natural Science Foundation of China(61425025)the Beijing Municipal Science and Technology Project(Z151100000915070 and Z171100000117008)。