麻醉准备室护理质量评价指标体系构建研究

目的:构建适用于麻醉准备室的护理质量评价指标体系,为监测麻醉准备室的护理质量提供依据。方法:基于文献调研法初步构建麻醉准备室护理质量评价指标体系框架,采用半结构访谈法初步构建指标体系,通过2轮德尔菲专家咨询确定护理质量评价指标体系。结果:开展了2轮专家函询,问卷有效回收率均达到100%,专家权威系数为0.945,肯德尔和谐系数分别为0.252和0.260;最终确立了一级指标3项、二级指标11项和三级指标66项,形成麻醉准备室护理质量评价指标体系。结论:麻醉准备室护理质量指标的构建具有科学性及可靠性,彰显麻醉准备室护理工作的特色,为麻醉准备室护理质量提供持续改进的评价方法。

蒙成药三臣方研究概况

基于蒙医传统理论对三臣方特定热证临床表征进行加减运用,且该方在中医、蒙医等临床亦有运用记录,使其具有十分重要的研究与理论价值。三臣方始见于《四部医典》,具有清热燥湿之效。通过蒙医药传统古籍文献资料、中国知网文献资料、万方数据库和维普官网信息源途径,从蒙剂“三臣方”化学成分、药理及功效方面开展文献综述研究,以期为该类蒙剂更深入的研究与开发应用提供参考。

Differentiation of Mesenchymal Stem Cells Into Dopaminergic Neuron-like Cells in vitro

To explore the way to induce mesenchymal stem cells (MSCs) to differentiate into dopaminergic neurons in vitro. Methods MSCs were obtained from rat bone marrow, cultured and passaged. MSCs used in this experiment had multipotency, which was indirectly proved by being induced to differentiate into chondrocytes and adipocytes. MSCs were cultured in medium containing 0.5 mmol/L IBMX for 2 days. Then the medium was replaced with induction medium, which contained GDNF, IL-1β, mesencephalic glial-cell-conditioned medium and flash-frozen mesencephalic membrane fragments. The surface markers of the differentiated neurons, such as NSE, nestin, MAP-2a, b and TH were detected by immunocytochemistry and Western blot after MSCs were cultured in induction medium for 7 days and 15 days. Results MSCs differentiated into neural progenitors and expressed nestin after MSCs were incubated with medium containing IBMX for 2 d. After the medium was replaced with induction medium containing many inducing agents, MSCs differentiated into neuron-like cells and dopaminergic neuron-like cells and expressed NSE, MAP-2a, b and TH. The percentage of NSE-positive cells, MAP-2a, b-positive cells and TH-positive cells was 30.032±2.489%, 41.580±5.101% and 34.958±5.534%, respectively after MSCs were induced in medium containing GDNF, IL-1β, mesencephalic glial-cell-conditioned medium and flash-frozen mesencephalic membrane fragments for 15 days. Conclusion MSCs can differentiate into dopaminergic neuron-like cells and are a new cell source for the treatment of neurodegeneration diseases and have a great potential for wide application

契丹文基本方向名称考释

经过多年的研究,表示方向名称“东、西、南、北”的契丹大、小字均已成功识别。但是这些方向名称的读音及其词源,一直未能得到较好的训释。契丹人的早期方向观念与现代地理学的方向观念相差90度,即契丹人把“东”作“南”,“西”作“北”,“南”作“西”,“北”作“东”。以此为前提,对契丹文方向词进行释读,提出“东”读■mud,“西”读d■ut~d■ult,“南”读der~dor,“北”读o■r~or,读音均类似于中古蒙古语的对应词汇。