The autophagy-lysosome pathway:a potential target in the chemical and gene therapeutic strategies for Parkinson’s disease

Parkinson’s disease is a common neurodegenerative disease with movement disorders associated with the intracytoplasmic deposition of aggregate proteins such asα-synuclein in neurons.As one of the major intracellular degradation pathways,the autophagy-lysosome pathway plays an important role in eliminating these proteins.Accumulating evidence has shown that upregulation of the autophagy-lysosome pathway may contribute to the clearance ofα-synuclein aggregates and protect against degeneration of dopaminergic neurons in Parkinson’s disease.Moreover,multiple genes associated with the pathogenesis of Parkinson’s disease are intimately linked to alterations in the autophagy-lysosome pathway.Thus,this pathway appears to be a promising therapeutic target for treatment of Parkinson’s disease.In this review,we briefly introduce the machinery of autophagy.Then,we provide a description of the effects of Parkinson’s disease–related genes on the autophagy-lysosome pathway.Finally,we highlight the potential chemical and genetic therapeutic strategies targeting the autophagy–lysosome pathway and their applications in Parkinson’s disease.

Exploration of Innovation Strategy in the Deep Integration of Information Technology and Education and Teaching

The rapid development of information technology provides a new opportunity and impetus for the reform of education and teaching.Deep integration of information technology and education and teaching is the only way to promote the modernization of education.Starting from the connotation of the deep integration of information technology and education and teaching,this paper analyzes the existing problems in the current integration process,and puts forward innovative strategies from the aspects of concept,resources,model,and evaluation.Through measures such as building a smart teaching environment,enriching high-quality teaching resources,innovating teaching organization models,and establishing multiple evaluation systems,we will realize the deep integration of information technology and teaching,promote the reform of teaching and learning methods,improve the quality of personnel training,and provide strong support for the modernization of education.

Comparative Study of Water-soluble and Alkali-soluble Hemicelluloses Extracted by Hydrothermal Pretreatment

The purpose of this study was to explore the differences of the hemicelluloses extracted by hydrothermal pretreatment using water and alkaline solutions(Na OH or KOH). The physicochemical properties and structural characteristics of two water-soluble and four alkali-soluble hemicelluloses extracted from the triploid of Populus tomentosa Carr. through the hydrothermal pretreatment were comparatively studied. It was observed that the alkalis(Na OH and KOH) were more effective than distilled water as extractants. Sugar analysis showed that xylose(66.83%~86.49%) was the major constituent, followed by glucose(6.83%~18.49%). Mannose(1.40%~8.42%), galactose(2.17%~4.05%), and arabinose(0.21%~2.26%) were also detected in the hemicellulosic fractions. The results of gelpermeation chromatography(GPC) indicated that the hemicelluloses extracted using the alkaline solutions had relatively higher molecular weights than those solubilized in distilled water. Further, based on spectroscopic ~1Hnuclear magnetic resonance(~1H-NMR) and two-dimensional heteronuclear singular quantum correlation(2D-HSQC) analyses, it was confirmed that the hemicellulosic fractions had a major structure of(1→4)-b-D-xylan and a minor structure of(1→4)-a-D-glucan with small amounts of substituted sugars and glucuronic acid attached.

Tip-enhanced photoluminescence spectroscopy of monolayer MoS_2

Probing the optical properties of molybdenum disulfide(MoS_2) is vital to its application in plasmon-enhanced spectroscopy, catalysts, sensing, and optoelectronic devices. In this paper, we theoretically studied the Raman and fluorescence properties of monolayer MoS_2 using tip-enhanced spectroscopy(TES). In the strong-coupling TES system, the Raman and fluorescence enhancement factors can be turned to as high as 4.5 × 10~8 and 3.3 × 10~3,respectively, by optimizing the tip–MoS_2-film distance. Our theoretical results not only help to deeply understand the TES properties of monolayer MoS_2, but also provide better guidance on the applications of the novel two-dimensional material.