A novel biomaterial scaffold was created from collagen chitosan/GAG. Its tensile strength was 8.6MPa(wet state)and degree of swelling water was 60%～75% with higer ultimate elongation 300%. Rabbit corneas of collagen ...A novel biomaterial scaffold was created from collagen chitosan/GAG. Its tensile strength was 8.6MPa(wet state)and degree of swelling water was 60%～75% with higer ultimate elongation 300%. Rabbit corneas of collagen chitosan/GAG implantation samples in vivo for biodegradation showed that the inplantion samples was complets biodegrable and digested afere 120 day. There was enought time to maintain cell growth,immigrating and proliferation. This biomaterials scaffold can be used for cell culture and in various tissue engineering fields.展开更多
The objective of this study was to produce the porous col lagen-chitosan/Gl ycosanminglycans(GAG) for corneal cell-seed implant as a t hree-dimensional tissue engineering scaffold to improve the regeneration cornea s....The objective of this study was to produce the porous col lagen-chitosan/Gl ycosanminglycans(GAG) for corneal cell-seed implant as a t hree-dimensional tissue engineering scaffold to improve the regeneration cornea s.The effect of various content of glycerol as form porous agent to collagen-ch i tosan/GAG preserved a porous dimensional structure was investigated.The heat-dr ying was used to prepare porous collagen-chitosan /GAG scaffold.The pore morpho logy of collagen-chitosan/GAG was controlled by changing the concentration of g lycerol solution and drying methods.The porous structure morphology was observed by SEM.The diameter of the pores form 10 to 50 μm.The highly porous scaffold had interconnecting pores.The corneal cell morphology was observed under the li ght microscope.These results suggest that collagen-chitosan/GAG showed that cor neal cell have formed confluent layers and resemble the surface of normal cornea l cell surface.展开更多
文摘A novel biomaterial scaffold was created from collagen chitosan/GAG. Its tensile strength was 8.6MPa(wet state)and degree of swelling water was 60%～75% with higer ultimate elongation 300%. Rabbit corneas of collagen chitosan/GAG implantation samples in vivo for biodegradation showed that the inplantion samples was complets biodegrable and digested afere 120 day. There was enought time to maintain cell growth,immigrating and proliferation. This biomaterials scaffold can be used for cell culture and in various tissue engineering fields.
文摘The objective of this study was to produce the porous col lagen-chitosan/Gl ycosanminglycans(GAG) for corneal cell-seed implant as a t hree-dimensional tissue engineering scaffold to improve the regeneration cornea s.The effect of various content of glycerol as form porous agent to collagen-ch i tosan/GAG preserved a porous dimensional structure was investigated.The heat-dr ying was used to prepare porous collagen-chitosan /GAG scaffold.The pore morpho logy of collagen-chitosan/GAG was controlled by changing the concentration of g lycerol solution and drying methods.The porous structure morphology was observed by SEM.The diameter of the pores form 10 to 50 μm.The highly porous scaffold had interconnecting pores.The corneal cell morphology was observed under the li ght microscope.These results suggest that collagen-chitosan/GAG showed that cor neal cell have formed confluent layers and resemble the surface of normal cornea l cell surface.
