生物钟基因能够参与调控植物的整个生命进程,对提高作物产量具有重要的作用。LNK1(NIGHTLIGHTINDUCIBLE AND CLOCK-REGULATED 1)、LNK2、RVE4(REVEILLE 4)、RVE8(REVEILLE 8)和TOC1(TIMING OF CAB EXPRESSION1)是植物中重要的生物钟基...生物钟基因能够参与调控植物的整个生命进程,对提高作物产量具有重要的作用。LNK1(NIGHTLIGHTINDUCIBLE AND CLOCK-REGULATED 1)、LNK2、RVE4(REVEILLE 4)、RVE8(REVEILLE 8)和TOC1(TIMING OF CAB EXPRESSION1)是植物中重要的生物钟基因。本研究利用BLAST同源比对和进化树分析的方法分别鉴定AtLNK1、AtLNK2、AtRVE4、AtRVE8和AtTOC1在大豆中的同源基因,通过qRT-PCR实验证明这些生物钟基因在大豆根、茎、叶等组织中均有表达。成功构建这些基因的CRISPR/Cas9敲除载体,并利用大豆根毛转化体系成功鉴定出13个基因的CRISPR/CAS9有效靶点。为进一步获得稳定的大豆突变体材料及研究其生物钟基因的功能提供了理论基础。展开更多
Outcrossing rate is an important determinant of cytoplasmic male sterile(CMS)breeding and hybrid seed production for heterosis in soybean.Parental lines with a high outcrossing rate were screened for backcross breedin...Outcrossing rate is an important determinant of cytoplasmic male sterile(CMS)breeding and hybrid seed production for heterosis in soybean.Parental lines with a high outcrossing rate were screened for backcross breeding to obtain the high outcrossing rate maintenance B-lines and sterile A-lines.Application in production practices will help to increase hybrid soybean production.In this study,JLCMS82B and JLCMS89B were selected as parents for the construction of outcrossing rate segregation populations,and the progeny-array approach(PAA)and glyphosate resistant gene markers were used to determine outcrossing rates.We found that:(1)The outcrossing rate between JLCMS82B and JLCMS89B was significantly different;(2)the outcrossing rate of the F2 segregating populations was a quantitative trait,though whether an additive or epistatic effect exists required analysis with a triple test intersection analysis;(3)agronomic traits correlated with outcrossing rate;outcrossing rate was the highest with plant height of about 84 cm,lower number of plant branches,earlier flowering time,larger angle between the branches and the main stem,and with more divergent plant morphology.Correlation analysis between agronomic traits and outcrossing rate can effectively guide the screening of parents with a high outcrossing rate.展开更多
The adaptability of soybean to be grown at a wide range of latitudes is attributed to natural variation in the major genes and quantitative trait loci （QTLs） that control flowering time and maturity. Thus, the ident...The adaptability of soybean to be grown at a wide range of latitudes is attributed to natural variation in the major genes and quantitative trait loci （QTLs） that control flowering time and maturity. Thus, the identification of genes controlling flowering time and maturity and the understanding of their molecular basis are critical for improving soybean productivity. However, due to the great effect of the major maturity gene E1 on flowering time, it is difficult to detect other small-effect QTLs. In this study, aiming to reduce the effect of the QTL, associated with the E1 gene, on the detection of other QTLs, we divided a population of 96 recombinant inbred lines （RILs） into two sub-populations： one with the E1 allele and another with the elns allele. Compared with the results of using all 96 recombinant inbred lines, additional QTLs for flowering time were identified in the sub-populations, two （qFT-B1 and qFT-H） in RILs with the E1 allele and one （qFT-J-2） in the RILs with the elnl allele, respectively. The three QTLs, qFT-B1, qFT-H and qFT-J-2 were true QTLs and played an important role in the regulation of growth period. Our data provides valuable information for the genetic mapping and gene cloning of traits controlling flowering time and maturity and will help a better understanding of the mechanism of photoperiod-regulated flowering and molecular breeding in soybean.展开更多
文摘生物钟基因能够参与调控植物的整个生命进程,对提高作物产量具有重要的作用。LNK1(NIGHTLIGHTINDUCIBLE AND CLOCK-REGULATED 1)、LNK2、RVE4(REVEILLE 4)、RVE8(REVEILLE 8)和TOC1(TIMING OF CAB EXPRESSION1)是植物中重要的生物钟基因。本研究利用BLAST同源比对和进化树分析的方法分别鉴定AtLNK1、AtLNK2、AtRVE4、AtRVE8和AtTOC1在大豆中的同源基因,通过qRT-PCR实验证明这些生物钟基因在大豆根、茎、叶等组织中均有表达。成功构建这些基因的CRISPR/Cas9敲除载体,并利用大豆根毛转化体系成功鉴定出13个基因的CRISPR/CAS9有效靶点。为进一步获得稳定的大豆突变体材料及研究其生物钟基因的功能提供了理论基础。
基金supported by the National Natural Science Foundation for Young Scientists of China (31301399)the Major Project for Science and Technology Development of Jilin Province, China (20170201001NY)the Agricultural Science Technology Innovation Project of Jilin Province, China (CXGC2017TD002)
文摘Outcrossing rate is an important determinant of cytoplasmic male sterile(CMS)breeding and hybrid seed production for heterosis in soybean.Parental lines with a high outcrossing rate were screened for backcross breeding to obtain the high outcrossing rate maintenance B-lines and sterile A-lines.Application in production practices will help to increase hybrid soybean production.In this study,JLCMS82B and JLCMS89B were selected as parents for the construction of outcrossing rate segregation populations,and the progeny-array approach(PAA)and glyphosate resistant gene markers were used to determine outcrossing rates.We found that:(1)The outcrossing rate between JLCMS82B and JLCMS89B was significantly different;(2)the outcrossing rate of the F2 segregating populations was a quantitative trait,though whether an additive or epistatic effect exists required analysis with a triple test intersection analysis;(3)agronomic traits correlated with outcrossing rate;outcrossing rate was the highest with plant height of about 84 cm,lower number of plant branches,earlier flowering time,larger angle between the branches and the main stem,and with more divergent plant morphology.Correlation analysis between agronomic traits and outcrossing rate can effectively guide the screening of parents with a high outcrossing rate.
基金partially supported by the National Natural Science Foundation of China (31430065, 31571686, 31201222 and 31371643)the Open Foundation of the Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences+5 种基金the “Hundred Talents” Program of the Chinese Academy of Sciencesthe Strategic Action Plan for Science and Technology Innovation of the Chinese Academy of Sciences (XDA08030108)the Natural Science Foundation of Heilongjiang Province, China (ZD201001, JC201313)the Research and Development of Applied Technology Project, Harbin, China (2014RFQYJ055)the Scientific Research Foundation for Returned Chinese Scholars of Heilongjiang Province, China (LC201417)the Science Foundation for Creative Research Talents of Harbin Science and Technology Bureau, China (2014RFQYJ046)
文摘The adaptability of soybean to be grown at a wide range of latitudes is attributed to natural variation in the major genes and quantitative trait loci （QTLs） that control flowering time and maturity. Thus, the identification of genes controlling flowering time and maturity and the understanding of their molecular basis are critical for improving soybean productivity. However, due to the great effect of the major maturity gene E1 on flowering time, it is difficult to detect other small-effect QTLs. In this study, aiming to reduce the effect of the QTL, associated with the E1 gene, on the detection of other QTLs, we divided a population of 96 recombinant inbred lines （RILs） into two sub-populations： one with the E1 allele and another with the elns allele. Compared with the results of using all 96 recombinant inbred lines, additional QTLs for flowering time were identified in the sub-populations, two （qFT-B1 and qFT-H） in RILs with the E1 allele and one （qFT-J-2） in the RILs with the elnl allele, respectively. The three QTLs, qFT-B1, qFT-H and qFT-J-2 were true QTLs and played an important role in the regulation of growth period. Our data provides valuable information for the genetic mapping and gene cloning of traits controlling flowering time and maturity and will help a better understanding of the mechanism of photoperiod-regulated flowering and molecular breeding in soybean.
