The deep-sea clam Calyptogena marissinica is widely distributed in the Haima cold seep ecosystem on the northwes-tern slope of the South China Sea with low pH values,low temperature and high pressure.Limited informati...The deep-sea clam Calyptogena marissinica is widely distributed in the Haima cold seep ecosystem on the northwes-tern slope of the South China Sea with low pH values,low temperature and high pressure.Limited information is available on the biomineralization of this species.In this research,we generated a comprehensive transcript dataset of C.marissinica’s mantle tissue,and a total of 19821 unigenes were assembled.Fourteen shell matrix proteins(SMP)-related genes were identified.The qPCR results showed that four out of six prismatic matrix genes(MSP2,MSP5,prisilkin-39,and shematrin),four out of the six nacreous matrix genes(perlucin,pif,pif97,and papilin),and two extrapallial fluid proteins(SPARC and calmodulin)were significantly expressed in the mantle.Both the nacreous and the prismatic layers are chrysanthemum-shaped,which are stacked on the top of each other to form a laminated nacreous structure.The alignment and phylogenetic analysis of MSP-5,Prisilkin-39,Perlucin,and Pif homologues showed that some amino acids of C.marissinica that differed from those detected in other molluscs may cause the different shape of the nacreous and prismatic layers,but do not lead to a change in the species’evolutionary status.These results indicated the conservation of the functions of SMP-related genes in C.marissinica,and the specific shape of the prismatic and nacreous layers of this deep-sea mollusc,which will contribute to the research on the molecular regulation mechanisms of biomineralization in C.marissinica and provide a new perspective to investigate biomineralization in deep-sea clams in general.展开更多
目的比较彩色多普勒超声(CDUS)和对比增强磁共振血管造影(CE-MRA)在评价肾动脉狭窄(RAS)中的应用价值。资料与方法回顾性分析50例RAS患者共100条肾动脉的CDUS及CE-MRA资料。所有患者最终均经数字减影血管造影(DSA)检查确认诊断。比较CDU...目的比较彩色多普勒超声(CDUS)和对比增强磁共振血管造影(CE-MRA)在评价肾动脉狭窄(RAS)中的应用价值。资料与方法回顾性分析50例RAS患者共100条肾动脉的CDUS及CE-MRA资料。所有患者最终均经数字减影血管造影(DSA)检查确认诊断。比较CDUS与CE-MRA对RAS检出率的差异。采用受试者工作特征(ROC)曲线评价CDUS与CE-MRA对RAS的诊断效能。结果CDUS与CE-MRA对RAS的检出率差异无统计学意义(57%比60.0%,P>0.05)。CDUS诊断RAS的ROC曲线下面积为0.88(95% CI 0.810.90),CE-MRA诊断RAS的ROC曲线下面积为0.79(95% CI 0.710.86),差异有统计学意义(Z=2.345,P<0.05)。结论CDUS及CE-MRA均具有较高的RAS检出率,其中CDUS具有更高的诊断效能。展开更多
目的旨在探讨糖尿病心肌病(DCM)早期心肌应变的改变特点。方法60只健康4周龄雄性C57BL/6J小鼠,采用随机数字法分为2型糖尿病(T2DM)组(30只)和对照组(30只)。对照组采用普通饲料喂养,T2DM组采用高脂饲料喂养。喂养4周后,T2DM组单次大剂...目的旨在探讨糖尿病心肌病(DCM)早期心肌应变的改变特点。方法60只健康4周龄雄性C57BL/6J小鼠,采用随机数字法分为2型糖尿病(T2DM)组(30只)和对照组(30只)。对照组采用普通饲料喂养,T2DM组采用高脂饲料喂养。喂养4周后,T2DM组单次大剂量腹腔注射链脲佐菌素(STZ)溶液制作T2DM模型,最终造模成功23只。对照组注射等剂量柠檬酸钠缓冲液。造模成功后第4、8、12、16、20和24周作为监测时间点采集实验数据。每个时间点时各组均入组9只小鼠,然后各组随机选取6只用于体重和血糖监测及7.0 T MR图像采集,处死剩余3只用于制作病理切片(天狼星红染色)以观察心肌间质纤维成分及微小血管的情况。MR图像采用心脏MR特征追踪(CMR-FT)技术进行分析,获得左心室整体周向应变峰值(GPCS)和径向应变峰值(GPRS),并测量射血分数(EF)等。观察期内,各组自身不同监测点参数间比较采用单因素方差分析;组间参数比较采取两独立样本t检验。结果观察期内组间血糖值差异均有统计学意义(P<0.05)。观察期(4~24周)内T2DM组小鼠GPCS呈下降趋势,组内差异有统计学意义(F=8.23,P<0.001);与对照组相比,T2DM组小鼠的GPCS于第20、24周时组间差异具有统计学意义。第20周时T2DM组GPCS为-11.4%±2.1%,对照组为-14.3%±1.9%(t=-2.54,P=0.029);第24周时T2DM组为-12.3%±1.7%,对照组为-14.6%±1.8%(t=-2.35,P=0.040)。EF在第24周时组间差异有统计学意义,T2DM组为51%±5%,对照组为62%±6%(t=3.38,P=0.007)。观察期内,T2DM组小鼠GPRS呈下降趋势,但组内和组间均差异无统计学意义(P>0.05)。心肌组织病理切片显示从第12周起T2DM组小鼠心肌间质纤维成分明显增多。结论T2DM小鼠早期即可出现心肌间质纤维化和心肌应变的改变,其中左心室整体周向应变峰值可较EF更早反映DCM的心功能改变。展开更多
基金supported by the Major Project of Basic and Applied Basic Research of Guangdong Province(No.2019B030302004)the Guangdong Basic and Applied Basic Research Foundation(No.2023A1515030295)the Science and Technology Planning Project of Guangdong Province,China(No.2020B1212060058).
文摘The deep-sea clam Calyptogena marissinica is widely distributed in the Haima cold seep ecosystem on the northwes-tern slope of the South China Sea with low pH values,low temperature and high pressure.Limited information is available on the biomineralization of this species.In this research,we generated a comprehensive transcript dataset of C.marissinica’s mantle tissue,and a total of 19821 unigenes were assembled.Fourteen shell matrix proteins(SMP)-related genes were identified.The qPCR results showed that four out of six prismatic matrix genes(MSP2,MSP5,prisilkin-39,and shematrin),four out of the six nacreous matrix genes(perlucin,pif,pif97,and papilin),and two extrapallial fluid proteins(SPARC and calmodulin)were significantly expressed in the mantle.Both the nacreous and the prismatic layers are chrysanthemum-shaped,which are stacked on the top of each other to form a laminated nacreous structure.The alignment and phylogenetic analysis of MSP-5,Prisilkin-39,Perlucin,and Pif homologues showed that some amino acids of C.marissinica that differed from those detected in other molluscs may cause the different shape of the nacreous and prismatic layers,but do not lead to a change in the species’evolutionary status.These results indicated the conservation of the functions of SMP-related genes in C.marissinica,and the specific shape of the prismatic and nacreous layers of this deep-sea mollusc,which will contribute to the research on the molecular regulation mechanisms of biomineralization in C.marissinica and provide a new perspective to investigate biomineralization in deep-sea clams in general.
文摘目的比较彩色多普勒超声(CDUS)和对比增强磁共振血管造影(CE-MRA)在评价肾动脉狭窄(RAS)中的应用价值。资料与方法回顾性分析50例RAS患者共100条肾动脉的CDUS及CE-MRA资料。所有患者最终均经数字减影血管造影(DSA)检查确认诊断。比较CDUS与CE-MRA对RAS检出率的差异。采用受试者工作特征(ROC)曲线评价CDUS与CE-MRA对RAS的诊断效能。结果CDUS与CE-MRA对RAS的检出率差异无统计学意义(57%比60.0%,P>0.05)。CDUS诊断RAS的ROC曲线下面积为0.88(95% CI 0.810.90),CE-MRA诊断RAS的ROC曲线下面积为0.79(95% CI 0.710.86),差异有统计学意义(Z=2.345,P<0.05)。结论CDUS及CE-MRA均具有较高的RAS检出率,其中CDUS具有更高的诊断效能。
文摘目的旨在探讨糖尿病心肌病(DCM)早期心肌应变的改变特点。方法60只健康4周龄雄性C57BL/6J小鼠,采用随机数字法分为2型糖尿病(T2DM)组(30只)和对照组(30只)。对照组采用普通饲料喂养,T2DM组采用高脂饲料喂养。喂养4周后,T2DM组单次大剂量腹腔注射链脲佐菌素(STZ)溶液制作T2DM模型,最终造模成功23只。对照组注射等剂量柠檬酸钠缓冲液。造模成功后第4、8、12、16、20和24周作为监测时间点采集实验数据。每个时间点时各组均入组9只小鼠,然后各组随机选取6只用于体重和血糖监测及7.0 T MR图像采集,处死剩余3只用于制作病理切片(天狼星红染色)以观察心肌间质纤维成分及微小血管的情况。MR图像采用心脏MR特征追踪(CMR-FT)技术进行分析,获得左心室整体周向应变峰值(GPCS)和径向应变峰值(GPRS),并测量射血分数(EF)等。观察期内,各组自身不同监测点参数间比较采用单因素方差分析;组间参数比较采取两独立样本t检验。结果观察期内组间血糖值差异均有统计学意义(P<0.05)。观察期(4~24周)内T2DM组小鼠GPCS呈下降趋势,组内差异有统计学意义(F=8.23,P<0.001);与对照组相比,T2DM组小鼠的GPCS于第20、24周时组间差异具有统计学意义。第20周时T2DM组GPCS为-11.4%±2.1%,对照组为-14.3%±1.9%(t=-2.54,P=0.029);第24周时T2DM组为-12.3%±1.7%,对照组为-14.6%±1.8%(t=-2.35,P=0.040)。EF在第24周时组间差异有统计学意义,T2DM组为51%±5%,对照组为62%±6%(t=3.38,P=0.007)。观察期内,T2DM组小鼠GPRS呈下降趋势,但组内和组间均差异无统计学意义(P>0.05)。心肌组织病理切片显示从第12周起T2DM组小鼠心肌间质纤维成分明显增多。结论T2DM小鼠早期即可出现心肌间质纤维化和心肌应变的改变,其中左心室整体周向应变峰值可较EF更早反映DCM的心功能改变。
