Brassica napus cultivars have little or no primary dormancy;however,they are prone to sec on dary dorma ncy in ducti on.Secondary dormant seeds can produce volunteer plants,which can result in genetic contamination,re...Brassica napus cultivars have little or no primary dormancy;however,they are prone to sec on dary dorma ncy in ducti on.Secondary dormant seeds can produce volunteer plants,which can result in genetic contamination,reduced quality and biosafety issues.However,informati on regarding the molecular mecha nism un derlyi ng sec on dary dormancy is limited.The MOTHER OF FT AND TFL1(MFT)gene,which is evolutionarily conserved in the plant kingdom,acts in a complex gene network in the seed dormancy or germination processes.In this study,we identified four B.napus genes that share high homology with AtMFT,named as BnaMFT.Analyses of c/s-acting elements showed that BnaMFT promoters contain multiple seed-specific regulatory elements,and various stress-and hormone-responsive elements.Further experiments validated that BnaMFTs were specifically expressed during seed maturation and in the dry seed,with peaks at 35-42 days after pollination.BnaMFTs were not sufficient for primary dormancy;however,they were significantly enhanced by secondary dormancy induction with PEG6000 treatment.Moreover,BnaMFTtranscripts were elevated by treatment with abscisic acid(ABA),which is known to be accumulated duri ng sec on dary dorma ncy.These results collectively suggest that increased BnaMFT tra nscription levels are associated with sec on dary dorma ncy inducti on in an ABA-depe ndent mariner in B.napus.展开更多
Development of new technologies for evaluating genetically modiifed （GM） crops has revealed that there are unintended insertions and expression changes in GM crops. Proifling techniques are non-targeted approaches a...Development of new technologies for evaluating genetically modiifed （GM） crops has revealed that there are unintended insertions and expression changes in GM crops. Proifling techniques are non-targeted approaches and are capable of detecting more unintended changes in GM crops. Here, we report the application of a comparative proteomic approach to investigate the protein proifle differences between a GM rice line, which has a lysine-rich protein gene, and its non-transgenic parental line. Proteome analysis by two-dimensional gel electrophoresis （2-DE） and mass spectrum analysis of the seeds identiifed 22 differentially expressed protein spots. Apart from a number of glutelins that were detected as targeted proteins in the GM line, the majority of the other changed proteins were involved in carbohydrate metabolism, protein synthesis and stress responses. These results indicated that the altered proteins were not associated with plant allergens or toxicity.展开更多
基金This work was funded by the National Natural Science Foundation of China(31470572 and 31701514)and the Qing Lan Project of Jiangsu Province,China(2016).
文摘Brassica napus cultivars have little or no primary dormancy;however,they are prone to sec on dary dorma ncy in ducti on.Secondary dormant seeds can produce volunteer plants,which can result in genetic contamination,reduced quality and biosafety issues.However,informati on regarding the molecular mecha nism un derlyi ng sec on dary dormancy is limited.The MOTHER OF FT AND TFL1(MFT)gene,which is evolutionarily conserved in the plant kingdom,acts in a complex gene network in the seed dormancy or germination processes.In this study,we identified four B.napus genes that share high homology with AtMFT,named as BnaMFT.Analyses of c/s-acting elements showed that BnaMFT promoters contain multiple seed-specific regulatory elements,and various stress-and hormone-responsive elements.Further experiments validated that BnaMFTs were specifically expressed during seed maturation and in the dry seed,with peaks at 35-42 days after pollination.BnaMFTs were not sufficient for primary dormancy;however,they were significantly enhanced by secondary dormancy induction with PEG6000 treatment.Moreover,BnaMFTtranscripts were elevated by treatment with abscisic acid(ABA),which is known to be accumulated duri ng sec on dary dorma ncy.These results collectively suggest that increased BnaMFT tra nscription levels are associated with sec on dary dorma ncy inducti on in an ABA-depe ndent mariner in B.napus.
基金supported by the National Major Special Project for the Development of Transgenic OrganismsChina(2009ZX-08011-003B)+2 种基金the Jiangsu Province Qing Lan Project for Young and Middle-Aged Academic LeadersChina and the Jiangsu Province Qing Lan Project for Outstanding Scientific and Technological Innovation TeamChina
文摘Development of new technologies for evaluating genetically modiifed （GM） crops has revealed that there are unintended insertions and expression changes in GM crops. Proifling techniques are non-targeted approaches and are capable of detecting more unintended changes in GM crops. Here, we report the application of a comparative proteomic approach to investigate the protein proifle differences between a GM rice line, which has a lysine-rich protein gene, and its non-transgenic parental line. Proteome analysis by two-dimensional gel electrophoresis （2-DE） and mass spectrum analysis of the seeds identiifed 22 differentially expressed protein spots. Apart from a number of glutelins that were detected as targeted proteins in the GM line, the majority of the other changed proteins were involved in carbohydrate metabolism, protein synthesis and stress responses. These results indicated that the altered proteins were not associated with plant allergens or toxicity.
