The solution structure of the imidazole adduct of oxidized horse heart cytochrome c(Im cyt c) has been determined through 2D NMR spectroscopy. The 35 conformers of the family show the RMSD values to the average struct...The solution structure of the imidazole adduct of oxidized horse heart cytochrome c(Im cyt c) has been determined through 2D NMR spectroscopy. The 35 conformers of the family show the RMSD values to the average structure of 0.063±0.007 nm for the backbone and 0.107±0.007 nm for all heavy atoms, respectively. The secondary structure elements and the overall folding of the present structure are substantially similar to those of the solution structure of native protein. However, the replacement of the axial ligand Met 80 with the exogenous imidazole ligand induces significant conformation changes in both backbone and side chains of the residues locating in the distal axial ligand regions. The electron delocalization on the heme moiety and the magnetic susceptibility tensor are consistent with these structural features.展开更多
目的:探讨非能量器械联合喉返神经监测在胸腔镜下肺癌根治术中保护喉返神经的可行性及安全性。方法:选取2020年3月至2022年4月于我院行单孔胸腔镜下肺癌根治术的非小细胞肺癌患者206例作为研究对象,分为使用能量器械清扫喉返神经相关淋...目的:探讨非能量器械联合喉返神经监测在胸腔镜下肺癌根治术中保护喉返神经的可行性及安全性。方法:选取2020年3月至2022年4月于我院行单孔胸腔镜下肺癌根治术的非小细胞肺癌患者206例作为研究对象,分为使用能量器械清扫喉返神经相关淋巴结的能量组和使用非能量器械并进行术中喉返神经监测的非能量监测组。回顾性分析两组患者的术中及术后情况。采用单变量分析和多变量分析来寻找喉返神经损伤的风险因素。结果:所有患者均顺利完成手术,无围术期死亡。喉返神经相关淋巴结的总转移率为6.96%(38/546)。能量组患者的手术时间明显短于非能量监测组(140 min vs 150 min,Z=-3.071,P=0.002),术后第一天引流量明显少于非能量组(170 mL vs 210 mL,Z=-2.017,P=0.044),但非能量监测组患者的喉返神经损伤明显少于能量组(7.48%vs 17.17%,χ^(2)=4.533,P=0.033),且非能量监测组的患者更有可能从喉返神经损伤中恢复(P=0.022)。体质指数(OR=1.228,95%CI:1.030~1.465,P=0.022)、肿瘤的解剖学分型(OR=0.079,95%CI:0.022~0.285,P<0.001)、肿瘤原发侧(OR=3.613,95%CI:1.130~11.550,P=0.030)和手术器械(OR=5.237,95%CI:1.686~16.266,P=0.004)是喉返神经损伤的独立风险因素。结论:体质指数、肿瘤的解剖学分型、肿瘤原发侧和手术器械是喉返神经损伤的独立风险因素。在单孔胸腔镜下肺癌根治术清扫喉返神经相关淋巴结时联合使用非能量器械和术中喉返神经监测可能有助于降低喉返神经损伤的发生率,并提高恢复率。展开更多
文摘The solution structure of the imidazole adduct of oxidized horse heart cytochrome c(Im cyt c) has been determined through 2D NMR spectroscopy. The 35 conformers of the family show the RMSD values to the average structure of 0.063±0.007 nm for the backbone and 0.107±0.007 nm for all heavy atoms, respectively. The secondary structure elements and the overall folding of the present structure are substantially similar to those of the solution structure of native protein. However, the replacement of the axial ligand Met 80 with the exogenous imidazole ligand induces significant conformation changes in both backbone and side chains of the residues locating in the distal axial ligand regions. The electron delocalization on the heme moiety and the magnetic susceptibility tensor are consistent with these structural features.
文摘目的:探讨非能量器械联合喉返神经监测在胸腔镜下肺癌根治术中保护喉返神经的可行性及安全性。方法:选取2020年3月至2022年4月于我院行单孔胸腔镜下肺癌根治术的非小细胞肺癌患者206例作为研究对象,分为使用能量器械清扫喉返神经相关淋巴结的能量组和使用非能量器械并进行术中喉返神经监测的非能量监测组。回顾性分析两组患者的术中及术后情况。采用单变量分析和多变量分析来寻找喉返神经损伤的风险因素。结果:所有患者均顺利完成手术,无围术期死亡。喉返神经相关淋巴结的总转移率为6.96%(38/546)。能量组患者的手术时间明显短于非能量监测组(140 min vs 150 min,Z=-3.071,P=0.002),术后第一天引流量明显少于非能量组(170 mL vs 210 mL,Z=-2.017,P=0.044),但非能量监测组患者的喉返神经损伤明显少于能量组(7.48%vs 17.17%,χ^(2)=4.533,P=0.033),且非能量监测组的患者更有可能从喉返神经损伤中恢复(P=0.022)。体质指数(OR=1.228,95%CI:1.030~1.465,P=0.022)、肿瘤的解剖学分型(OR=0.079,95%CI:0.022~0.285,P<0.001)、肿瘤原发侧(OR=3.613,95%CI:1.130~11.550,P=0.030)和手术器械(OR=5.237,95%CI:1.686~16.266,P=0.004)是喉返神经损伤的独立风险因素。结论:体质指数、肿瘤的解剖学分型、肿瘤原发侧和手术器械是喉返神经损伤的独立风险因素。在单孔胸腔镜下肺癌根治术清扫喉返神经相关淋巴结时联合使用非能量器械和术中喉返神经监测可能有助于降低喉返神经损伤的发生率,并提高恢复率。
