Comparative study on the activity and kinectic properties of acid phosphatase （ACPase） of three soils amended with Hg and Cu at constant temperature and humidity was carried out. The results indicated that the inhib...Comparative study on the activity and kinectic properties of acid phosphatase （ACPase） of three soils amended with Hg and Cu at constant temperature and humidity was carried out. The results indicated that the inhibition on ACPase of the three sample soils by Hg and Cu varied with the content of soil organic matter and pH, where, Soil 1 was the most seriously contaminated due to its lowest content of organic matter and the lowest pH among three samples, Soil 2 took the second place, and Soil 3 was the least contaminated. Except Soil 3, the activity of soil ACPase tended to increase along with the contact time under the same type and the same concentration of heavy metal. In particular the Vmax values of ACPase in all three samples decreased with increasing Hg and Cu concentration, whereas the Km values were affected weakly. According to the change of Vmax and Km values, Cu and Hg had the same inhibition effect on soil ACPase. Both of them may he a type of compound of non-competitive and anti-competitive inhibition. Statistic analyses indicated that activities of soil ACPase and Vmax values could serve as bioindicator to partially denote the heavy metal Hg and Cu contamination degree.展开更多
[ Objective] To explore the effects of synergy between earthworms （ Eisenia foetida） and effective microorganisms （EM） on vermicom. posting of pig manure and to provide a new idea for rational and effective utiliz...[ Objective] To explore the effects of synergy between earthworms （ Eisenia foetida） and effective microorganisms （EM） on vermicom. posting of pig manure and to provide a new idea for rational and effective utilization and treatment of animal manure. [Method] Four treatments were used to compost pig manure, namely, EM-inoculated manure, earthworms-inoculated manure, EM and earthworms-inoculated manure and control （CK） manure. The experiment lasted for 60 d. On Day 0, 15, 30, 45 and 60, the pig manure was collected, and the content of total organic carbon （TOC）, total Kjeldahl nitrogen （TKN）, ammonium nitrogen, nitrate nitrogen and water soluble carbon （WSC） was determined, respectively. [Resuit] Compared with the control, the EM and earthworms-inoculated manure had a decrease of 42.8% in the TOC content, an increase of 13.6% in the TKN content, a decrease of 49.5% in the carbon nitrogen ratio, the conversion rate of ammonium nitrogen of 98.1%, a 96 times increase in nitrate nitrogen, the ratio of nitrate nitrogen to ammonium nitrogen of 61, and a decrease of 58.9% in the WSC content. [ Conclusion] The synergy between earthworms and EM can accelerate maturation of pig manure and improve mineralization and stability of products.展开更多
基金Project (No. 2002CB410800) supported by the National NaturalScience Foundation of China
文摘Comparative study on the activity and kinectic properties of acid phosphatase （ACPase） of three soils amended with Hg and Cu at constant temperature and humidity was carried out. The results indicated that the inhibition on ACPase of the three sample soils by Hg and Cu varied with the content of soil organic matter and pH, where, Soil 1 was the most seriously contaminated due to its lowest content of organic matter and the lowest pH among three samples, Soil 2 took the second place, and Soil 3 was the least contaminated. Except Soil 3, the activity of soil ACPase tended to increase along with the contact time under the same type and the same concentration of heavy metal. In particular the Vmax values of ACPase in all three samples decreased with increasing Hg and Cu concentration, whereas the Km values were affected weakly. According to the change of Vmax and Km values, Cu and Hg had the same inhibition effect on soil ACPase. Both of them may he a type of compound of non-competitive and anti-competitive inhibition. Statistic analyses indicated that activities of soil ACPase and Vmax values could serve as bioindicator to partially denote the heavy metal Hg and Cu contamination degree.
基金funded by the Scientific Research Foundation of Changsha University (CDJJ-07010107)
文摘[ Objective] To explore the effects of synergy between earthworms （ Eisenia foetida） and effective microorganisms （EM） on vermicom. posting of pig manure and to provide a new idea for rational and effective utilization and treatment of animal manure. [Method] Four treatments were used to compost pig manure, namely, EM-inoculated manure, earthworms-inoculated manure, EM and earthworms-inoculated manure and control （CK） manure. The experiment lasted for 60 d. On Day 0, 15, 30, 45 and 60, the pig manure was collected, and the content of total organic carbon （TOC）, total Kjeldahl nitrogen （TKN）, ammonium nitrogen, nitrate nitrogen and water soluble carbon （WSC） was determined, respectively. [Resuit] Compared with the control, the EM and earthworms-inoculated manure had a decrease of 42.8% in the TOC content, an increase of 13.6% in the TKN content, a decrease of 49.5% in the carbon nitrogen ratio, the conversion rate of ammonium nitrogen of 98.1%, a 96 times increase in nitrate nitrogen, the ratio of nitrate nitrogen to ammonium nitrogen of 61, and a decrease of 58.9% in the WSC content. [ Conclusion] The synergy between earthworms and EM can accelerate maturation of pig manure and improve mineralization and stability of products.
