为了实现鲢鱼加工副产物的高效综合利用,以鲢鱼精巢为原料,研究柠檬酸浓度、提取温度、超声功率、提取时间、固液比、提取次数对鱼精蛋白得率的影响。在单因素试验基础上,通过正交试验获得最佳提取工艺为:柠檬酸浓度0.45mol/L,温度55℃...为了实现鲢鱼加工副产物的高效综合利用,以鲢鱼精巢为原料,研究柠檬酸浓度、提取温度、超声功率、提取时间、固液比、提取次数对鱼精蛋白得率的影响。在单因素试验基础上,通过正交试验获得最佳提取工艺为:柠檬酸浓度0.45mol/L,温度55℃,超声时间20min,超声功率400 W,固液比130(g/mL),提取3次。在最佳工艺条件下,鱼精蛋白得率为3.25%;鱼精粗蛋白经Sepharose Fast Flow离子交换层析分离得到4个组分,对4个组分进行抗菌性分析可知,除鱼精蛋白的前体蛋白外,其他组分对沙门氏菌、金黄色葡萄球菌和大肠杆菌均具有较好的抑制活性,其中组分II、III的抑菌活性较好。展开更多
2,4,6-三芳基吡啶是一类重要的杂环化合物,在多个领域有广泛的应用。本文在氯化胆碱/对甲苯磺酸组成的低共熔溶剂中,以芳香酮、芳香醛、乙酸铵为原料,微波辐射下一锅合成了一系列2,4,6-三芳基吡啶,产率74.6~90.7%,产物结构经^1H NMR、^1...2,4,6-三芳基吡啶是一类重要的杂环化合物,在多个领域有广泛的应用。本文在氯化胆碱/对甲苯磺酸组成的低共熔溶剂中,以芳香酮、芳香醛、乙酸铵为原料,微波辐射下一锅合成了一系列2,4,6-三芳基吡啶,产率74.6~90.7%,产物结构经^1H NMR、^13 C NMR表征。该法具有反应条件温和、操作简单、产率较高、耗时短、绿色环保等优势。展开更多
The authors dealt with the effects of dynamic high pressure micro-fluidization（untreated and 20-160 MPa treated） on the structure and physico-chemical properties of egg white solutions[6%（mass fraction） or 61.2 mg...The authors dealt with the effects of dynamic high pressure micro-fluidization（untreated and 20-160 MPa treated） on the structure and physico-chemical properties of egg white solutions[6%（mass fraction） or 61.2 mg protein/mL]. Micro-fluidization treatment resulted in a decrease in mean particle size, and little change in solubility. It was found that the residual denaturation enthalpy was decreased with the increase of pressure from 20 to 100 MPa and from 120 to 160 MPa, reaching into the maximum at 160 MPa. There was an increase of surface hydrophobicity with the maximum at 100 MPa observed. Simultaneously, micro-fluidization treatment also resulted in a decrease in the content of sulfhydryl groups.展开更多
文摘为了实现鲢鱼加工副产物的高效综合利用,以鲢鱼精巢为原料,研究柠檬酸浓度、提取温度、超声功率、提取时间、固液比、提取次数对鱼精蛋白得率的影响。在单因素试验基础上,通过正交试验获得最佳提取工艺为:柠檬酸浓度0.45mol/L,温度55℃,超声时间20min,超声功率400 W,固液比130(g/mL),提取3次。在最佳工艺条件下,鱼精蛋白得率为3.25%;鱼精粗蛋白经Sepharose Fast Flow离子交换层析分离得到4个组分,对4个组分进行抗菌性分析可知,除鱼精蛋白的前体蛋白外,其他组分对沙门氏菌、金黄色葡萄球菌和大肠杆菌均具有较好的抑制活性,其中组分II、III的抑菌活性较好。
文摘2,4,6-三芳基吡啶是一类重要的杂环化合物,在多个领域有广泛的应用。本文在氯化胆碱/对甲苯磺酸组成的低共熔溶剂中,以芳香酮、芳香醛、乙酸铵为原料,微波辐射下一锅合成了一系列2,4,6-三芳基吡啶,产率74.6~90.7%,产物结构经^1H NMR、^13 C NMR表征。该法具有反应条件温和、操作简单、产率较高、耗时短、绿色环保等优势。
基金Supported by the National Natural Science Foundation of China(No.20666004)Program for Changjiang Scholars and Innovative Research Team in University of China(No.IRT0540)
文摘The authors dealt with the effects of dynamic high pressure micro-fluidization（untreated and 20-160 MPa treated） on the structure and physico-chemical properties of egg white solutions[6%（mass fraction） or 61.2 mg protein/mL]. Micro-fluidization treatment resulted in a decrease in mean particle size, and little change in solubility. It was found that the residual denaturation enthalpy was decreased with the increase of pressure from 20 to 100 MPa and from 120 to 160 MPa, reaching into the maximum at 160 MPa. There was an increase of surface hydrophobicity with the maximum at 100 MPa observed. Simultaneously, micro-fluidization treatment also resulted in a decrease in the content of sulfhydryl groups.
