目的探讨莫洛尼病毒前病毒整合位点-1(proviral integration site of moloney virus-1,PIM-1)和程序性死亡-配体1(programmed death-ligand 1,PD-L1)在乳腺癌组织中表达的相关性并在细胞系中验证PIM-1对PD-L1表达的影响。方法制备208例...目的探讨莫洛尼病毒前病毒整合位点-1(proviral integration site of moloney virus-1,PIM-1)和程序性死亡-配体1(programmed death-ligand 1,PD-L1)在乳腺癌组织中表达的相关性并在细胞系中验证PIM-1对PD-L1表达的影响。方法制备208例乳腺癌组织芯片,采用免疫组化SP法检测乳腺癌组织中PIM-1和PD-L1的表达水平,采用H-Score评分和χ^(2)检验分析两者表达的相关性。在乳腺癌细胞系MCF-7和MDA-MB-231中过表达或敲减PIM-1,应用qRT-PCR和Western blot法检测PD-L1的表达变化。结果PD-L1在三阴型乳腺癌中的阳性率为13.16%,显著高于Luminal A型(4.65%)、Luminal B型(2.12%)和HER-2过表达型(4.76%)(P=0.029)。PIM-1高表达组中PD-L1的阳性率(12.50%)显著高于PIM-1低表达组(1.92%)(P=0.006)。细胞学实验结果显示,过表达PIM-1可显著促进乳腺癌细胞中PD-L1的表达,敲减PIM-1则明显降低PD-L1的表达水平。结论乳腺癌组织中PD-L1的阳性率与PIM-1的表达呈正相关,PIM-1是PD-L1的正向调控因子。PIM-1可能成为乳腺癌免疫治疗的潜在靶点。展开更多
Two new coordination polymers,named {[Co3（pfca）6（phen）2]·2H2O}n（1） and[Cu（pfca）2（4,4＇-bpy）（H2O）]n（2）（Hpfca = 4-fluorocinnamic acid and 4,4-bpy = 4,4-bipyridine）,were hydrothermally synthesized an...Two new coordination polymers,named {[Co3（pfca）6（phen）2]·2H2O}n（1） and[Cu（pfca）2（4,4＇-bpy）（H2O）]n（2）（Hpfca = 4-fluorocinnamic acid and 4,4-bpy = 4,4-bipyridine）,were hydrothermally synthesized and characterized by elemental analysis,IR spectroscopy,thermogravimetric analysis（TGA）,and single-crystal X-ray diffraction.Complex 1 crystallizes in the triclinic crystal system,space group P1 with a = 10.939（3）,b = 12.430（4）,c = 13.815（4） A,α=78.436（3）,β= 70.261（3）,γ= 80.268（3）°,V= 1721.8（9） A^3,Dc = 1.508 g/cm^3,Mr= 1564.06,Z = 1,F（000） = 799,μ= 0.802 mm^（-1）,the final R = 0.0432 and wR = 0.1015 for 13250 observed reflections with I 2σ（I）.Complex 2 is of monoclinic system,space group P2／lc with a =15.4985（14）,b = 15.8424（14）,c = 10.9528（10） A,β= 107.1600（10）°,V= 2569.6（4） A^3,Dc = 1.468g/cm^3,Mr = 568.02,Z = 4,F（000） = 1164,μ= 0.906 mm^（-1）,the final R = 0.0371 and wR = 0.0842 for 18183 observed reflections with I〉 2σ（I）.Complex 1 features linear Co（Ⅱ）-trinuclear units,which are assembled into a final 3D supramolecular structure through π…π stacking interactions and F-H weak interactions.Complex 2 consists of 1D polymeric Cu（Ⅱ）-chains that are further cohered together by extensive H-bonds.展开更多
The cyanide groups of letrozole(1-[bis(4-cyanophenyl)methyl]-1,2,4-triazole) were hydrolyzed into the carboxylic groups under alkaline conditions. Then the hydrolysis product(1-[bis(4-carboxylphenyl)methyl]-1,2,4-tria...The cyanide groups of letrozole(1-[bis(4-cyanophenyl)methyl]-1,2,4-triazole) were hydrolyzed into the carboxylic groups under alkaline conditions. Then the hydrolysis product(1-[bis(4-carboxylphenyl)methyl]-1,2,4-triazole, H2ltzda) reacted with Co(NO3)2·6H2O in the presence of different bipyridl-typed ligands under hydrothermal conditions. As a result, two new Co(Ⅱ) MOFs were obtained, formulated as [Co2(ltzda)2(bpp)2]n(1) and {[Co2(ltzda)2(bpy)]·4H2O}n(2)(bpp = 1,3-di(4-pyridyl)propane, bpy = 4,4’-bipyridine). Two MOFs have been characterized by single-crystal X-ray diffraction, thermogravimetric analysis and magnetic measure. 1 displays a 2D metal-organic framework containing wave layers and 2 shows a pillared-layer structure existing in a 2-fold interpenetrated mode.展开更多
Objective:To evaluate the efficacy and safety of Yishen-Huoxue Formula in the treatment of refractory nephrotic syndrome(RNS)using evidence-based medicine.Methods:Databases CNKI,WanFang,VIP,CBM,PubMed,EMbase,and Cochr...Objective:To evaluate the efficacy and safety of Yishen-Huoxue Formula in the treatment of refractory nephrotic syndrome(RNS)using evidence-based medicine.Methods:Databases CNKI,WanFang,VIP,CBM,PubMed,EMbase,and Cochrane Library were searched for randomized controlled trials(RCTs)on the treatment of RNS with the Yishen-Huoxue Formula from June 2008 to July 2020.The quality of the literature was evaluated by the Cochrane.Meta-analysis was performed using Review Manager 5.3 software.Results:A total of 18 articles and 1432 patients were included in this study.The results showed compared to the control group,Yishen-Huoxue Formula was much better in improving total effectiverate[OR=4.15,(95%CI:3.03,5.68),P<0.05],plasma albumin[MD=5.08,(95%CI:3.42,6.74),P<0.05]and decreasing 24-h urine protein quantitation[MD=-0.99,(95%CI:-1.30,-0,69),P<0.05],recurrence rate[OR=0.21,95%CI(0.11,0.40),P<0.05],and adverse reaction rate[OR=0.33,95%CI(0.21,0.52),P<0.05].However,the improvement of complete remission rate was affected by the course of treatment.The effects were similar in less than or equal to nine weeks[OR=1.52,(95%CI:0.74,3.13),P=0.25],whereas the experimental group was superior to the control group in 12-24 weeks[OR=2.47,(95%CI:1.63,3.74),P<0.05]and more than or equal to 26 weeks[OR=2.04,(95%CI:1.43,2.91),P<0.05].Conclusion:The efficacy and safety of Yishen-Huoxue Formula for refractory nephrotic syndrome is better than that of the Western medicine group,but the exact efficacy still needs to be confirmed by prospective studies of higher quality.展开更多
By adopting mixed-ligand strategy,two Co(Ⅱ)coordination polymers,[Co(phda)(itmb)(H2O)2]n(1)and[Co(phda)(Hpytz)]n(2)(H2phda=1,2-phenylenediacetate,itmb=1-(imidazo-1-ly)-4-(1,2,4-triazol-1-yl methyl)benzene and Hpytz=3...By adopting mixed-ligand strategy,two Co(Ⅱ)coordination polymers,[Co(phda)(itmb)(H2O)2]n(1)and[Co(phda)(Hpytz)]n(2)(H2phda=1,2-phenylenediacetate,itmb=1-(imidazo-1-ly)-4-(1,2,4-triazol-1-yl methyl)benzene and Hpytz=3,5-di(4-pyridyl)-1,2,4-triazolate),were hydrothermally synthesized and then characterized by elemental analysis,IR spectroscopy,thermogravimetric analysis(TGA),powder X-ray diffraction(PXRD)and single-crystal X-ray diffraction.The single-crystal X-ray diffraction reveals that two complexes feature carboxylato-bridged binuclear subunits extended further by the nitrogen-rich coligands to form different structures.Complex 1 containing phda-bridged binuclear cobalt is developed by itmb coligand to 1D double-stranded chain,whereas complex 2 exhibits 2D open bilayers,which result from the ribbon-like carboxylate chains pillared Hpytz coligands.In addition,the magnetic susceptibilities of both compounds were measured over the temperature range of 3~300 K,and the data were analyzed well using Mag Saki software.The best fitting parameters wereκ=0.98,λ=-100 cm-1,?=588 cm-1 and J=0.6 cm-1 for complex 1 andκ=0.86,λ=-110 cm-1,?=756.8 cm-1 and J=-1.25 cm-1 for complex 2.The results indicate the existence of weaker ferromagnetic interaction for complex 1 and antiferromagnetic interaction for complex 2 between the metal centers in the dinuclear metal units.展开更多
基金Supported by the Program for Science&Technology Innovation Talents in Universities of Henan Province(No.14HASTIT017)the Program for Innovative Research Team(in Science and Technology)in University of Henan Province(No.14IRTSTHN008)+1 种基金the Foundation of Education Committee of Henan Province(No.142300410301)the National Natural Science Foundation of China(No.21571093)
文摘Two new coordination polymers,named {[Co3（pfca）6（phen）2]·2H2O}n（1） and[Cu（pfca）2（4,4＇-bpy）（H2O）]n（2）（Hpfca = 4-fluorocinnamic acid and 4,4-bpy = 4,4-bipyridine）,were hydrothermally synthesized and characterized by elemental analysis,IR spectroscopy,thermogravimetric analysis（TGA）,and single-crystal X-ray diffraction.Complex 1 crystallizes in the triclinic crystal system,space group P1 with a = 10.939（3）,b = 12.430（4）,c = 13.815（4） A,α=78.436（3）,β= 70.261（3）,γ= 80.268（3）°,V= 1721.8（9） A^3,Dc = 1.508 g/cm^3,Mr= 1564.06,Z = 1,F（000） = 799,μ= 0.802 mm^（-1）,the final R = 0.0432 and wR = 0.1015 for 13250 observed reflections with I 2σ（I）.Complex 2 is of monoclinic system,space group P2／lc with a =15.4985（14）,b = 15.8424（14）,c = 10.9528（10） A,β= 107.1600（10）°,V= 2569.6（4） A^3,Dc = 1.468g/cm^3,Mr = 568.02,Z = 4,F（000） = 1164,μ= 0.906 mm^（-1）,the final R = 0.0371 and wR = 0.0842 for 18183 observed reflections with I〉 2σ（I）.Complex 1 features linear Co（Ⅱ）-trinuclear units,which are assembled into a final 3D supramolecular structure through π…π stacking interactions and F-H weak interactions.Complex 2 consists of 1D polymeric Cu（Ⅱ）-chains that are further cohered together by extensive H-bonds.
基金supported by the Project of Science&Technology in Henan Province(No.172102310441)the National Natural Science Foundation of China(No.21571093)
文摘The cyanide groups of letrozole(1-[bis(4-cyanophenyl)methyl]-1,2,4-triazole) were hydrolyzed into the carboxylic groups under alkaline conditions. Then the hydrolysis product(1-[bis(4-carboxylphenyl)methyl]-1,2,4-triazole, H2ltzda) reacted with Co(NO3)2·6H2O in the presence of different bipyridl-typed ligands under hydrothermal conditions. As a result, two new Co(Ⅱ) MOFs were obtained, formulated as [Co2(ltzda)2(bpp)2]n(1) and {[Co2(ltzda)2(bpy)]·4H2O}n(2)(bpp = 1,3-di(4-pyridyl)propane, bpy = 4,4’-bipyridine). Two MOFs have been characterized by single-crystal X-ray diffraction, thermogravimetric analysis and magnetic measure. 1 displays a 2D metal-organic framework containing wave layers and 2 shows a pillared-layer structure existing in a 2-fold interpenetrated mode.
基金Shanxi Province Science and Technology Research Project(2016ZD0307)Jinyao Comprehensive Development and Utilization Collaborative Innovation Center Project(2017-JYXT-32)+2 种基金The Key Project of Shanxi Province Key R&D Program(201603D3113023)The Key R&D Program(Guide)Project of Shanxi Province(201703D421024)Graduate Education Innovation Program of Shanxi University of Traditional Chinese Medicine(2020JG001)。
文摘Objective:To evaluate the efficacy and safety of Yishen-Huoxue Formula in the treatment of refractory nephrotic syndrome(RNS)using evidence-based medicine.Methods:Databases CNKI,WanFang,VIP,CBM,PubMed,EMbase,and Cochrane Library were searched for randomized controlled trials(RCTs)on the treatment of RNS with the Yishen-Huoxue Formula from June 2008 to July 2020.The quality of the literature was evaluated by the Cochrane.Meta-analysis was performed using Review Manager 5.3 software.Results:A total of 18 articles and 1432 patients were included in this study.The results showed compared to the control group,Yishen-Huoxue Formula was much better in improving total effectiverate[OR=4.15,(95%CI:3.03,5.68),P<0.05],plasma albumin[MD=5.08,(95%CI:3.42,6.74),P<0.05]and decreasing 24-h urine protein quantitation[MD=-0.99,(95%CI:-1.30,-0,69),P<0.05],recurrence rate[OR=0.21,95%CI(0.11,0.40),P<0.05],and adverse reaction rate[OR=0.33,95%CI(0.21,0.52),P<0.05].However,the improvement of complete remission rate was affected by the course of treatment.The effects were similar in less than or equal to nine weeks[OR=1.52,(95%CI:0.74,3.13),P=0.25],whereas the experimental group was superior to the control group in 12-24 weeks[OR=2.47,(95%CI:1.63,3.74),P<0.05]and more than or equal to 26 weeks[OR=2.04,(95%CI:1.43,2.91),P<0.05].Conclusion:The efficacy and safety of Yishen-Huoxue Formula for refractory nephrotic syndrome is better than that of the Western medicine group,but the exact efficacy still needs to be confirmed by prospective studies of higher quality.
基金supported by the National Natural Science Foundation of China(No.21571093)Colleges and Universities Key Scientific Research Project of Henan Province(No.18B150016)。
文摘By adopting mixed-ligand strategy,two Co(Ⅱ)coordination polymers,[Co(phda)(itmb)(H2O)2]n(1)and[Co(phda)(Hpytz)]n(2)(H2phda=1,2-phenylenediacetate,itmb=1-(imidazo-1-ly)-4-(1,2,4-triazol-1-yl methyl)benzene and Hpytz=3,5-di(4-pyridyl)-1,2,4-triazolate),were hydrothermally synthesized and then characterized by elemental analysis,IR spectroscopy,thermogravimetric analysis(TGA),powder X-ray diffraction(PXRD)and single-crystal X-ray diffraction.The single-crystal X-ray diffraction reveals that two complexes feature carboxylato-bridged binuclear subunits extended further by the nitrogen-rich coligands to form different structures.Complex 1 containing phda-bridged binuclear cobalt is developed by itmb coligand to 1D double-stranded chain,whereas complex 2 exhibits 2D open bilayers,which result from the ribbon-like carboxylate chains pillared Hpytz coligands.In addition,the magnetic susceptibilities of both compounds were measured over the temperature range of 3~300 K,and the data were analyzed well using Mag Saki software.The best fitting parameters wereκ=0.98,λ=-100 cm-1,?=588 cm-1 and J=0.6 cm-1 for complex 1 andκ=0.86,λ=-110 cm-1,?=756.8 cm-1 and J=-1.25 cm-1 for complex 2.The results indicate the existence of weaker ferromagnetic interaction for complex 1 and antiferromagnetic interaction for complex 2 between the metal centers in the dinuclear metal units.
