Objective:To investigate the effect of Wujiadan Recipe on PLC-β1,IP3,IP3R,Cam,PLC-β1,IP3R1 mRNA and GnRH,Cam protein from hypothalamus tissue of ovariectomized female rats,and to further clarify whether Wujiadan Rec...Objective:To investigate the effect of Wujiadan Recipe on PLC-β1,IP3,IP3R,Cam,PLC-β1,IP3R1 mRNA and GnRH,Cam protein from hypothalamus tissue of ovariectomized female rats,and to further clarify whether Wujiadan Recipe plays a role through PLC-β/IP3/CaM signal pathway.Methods:We randomly divided 64 SD adult female rats into NG group(normal group),MG group(the model group),TMC group(the control group with traditional Chinese medicine),WMC group(the control group with western medicine)with 10 rats in each,and Wujiadan Recipe high(WH),Wujiadan Recipe medium(WM)and Wujiadan Recipe low dose group(WL)with 8 rats in each.Except the NG group,we removed the ovarian tissues of other six groups to establish the ovariectomized model.When the model is successfully replicated,we give the medicine to the rats in each group once a day.The TMC and WMC group were given the corresponding dose of Gengnian'an tablet suspension and Bujiale suspension by gavage respectively.The NG and MG group were given equal measure of normal saline.After 4 weeks,the rats were killed.We use ELISA to detecte the levels of PLC-β1,IP3,IP3R and Cam;and we use PCR to detecte the mRNA expression levels of PLC-βand IP3R1;and use WesternBlot to detecte the protein expression levels of Cam and GnRH in rat hypothalamus tissue.Results:The test results of PLC-β1,IP3,IP3R and Cam in the hypothalamus homogenate of the model group were significantly increased than the NG group(P<0.01).The results of PLC-β1 and IP3R1 and GnRHmRNA were significantly increased(P<0.01),and the expressions of GnRH and Cam protein were higher than NG(P<0.01).Wujiadan Recipe could reduce the results of PLC-β1,IP3,IP3R and Cam in hypothalamus of ovariectomized rats,and reduce the expression of PLC-β1 and IP3R1 and GnRH mRNA.Conclusion:Wujiadan Recipe down-regulates GnRH expression,which may be caused by acting on PLC-β1,IP3,IP3R,CaM in hypothalamus of ovariectomized rats,so as to coordinate the function of reproductive axis and improve the reproductive endocrine level of patients with menopausal syndrome.展开更多
采用“二水法”湿法磷酸工艺对阿联酋某中品位磷矿进行了制酸评价研究,通过单因素实验得到了“二水法”湿法磷酸最适宜的生产控制条件,包括游离SO_(3)浓度、磷酸浓度、停留时间和液固比4个主要工艺参数。磷矿制酸系统在最适宜工艺参数...采用“二水法”湿法磷酸工艺对阿联酋某中品位磷矿进行了制酸评价研究,通过单因素实验得到了“二水法”湿法磷酸最适宜的生产控制条件,包括游离SO_(3)浓度、磷酸浓度、停留时间和液固比4个主要工艺参数。磷矿制酸系统在最适宜工艺参数控制下连续运行24 h,磷石膏滤饼采用二级逆流洗涤,磷矿中P_(2)O_(5)转化率可达到97.9%,回收率可达到96.9%,料浆的过滤强度约为10.5 t P_(2)O_(5)每平方米每天(TPD P_(2)O_(5)/m^(2))。展开更多
Alkylation of the ketimine 2 obtained from condensation of (+)-ketopinic acid and benzyla- mine with a variety of alkylating agents gives the products whose trends in diastereomeric excesses (1- 100% O. P.) appear to ...Alkylation of the ketimine 2 obtained from condensation of (+)-ketopinic acid and benzyla- mine with a variety of alkylating agents gives the products whose trends in diastereomeric excesses (1- 100% O. P.) appear to correlate with the structure and reactivity of electrophilic agents. Using excess n-butyl lithium and allylic or benzylic halides, β-alkylation occured.展开更多
基金Health Commission of Heilongjiang Province(2019-085)。
文摘Objective:To investigate the effect of Wujiadan Recipe on PLC-β1,IP3,IP3R,Cam,PLC-β1,IP3R1 mRNA and GnRH,Cam protein from hypothalamus tissue of ovariectomized female rats,and to further clarify whether Wujiadan Recipe plays a role through PLC-β/IP3/CaM signal pathway.Methods:We randomly divided 64 SD adult female rats into NG group(normal group),MG group(the model group),TMC group(the control group with traditional Chinese medicine),WMC group(the control group with western medicine)with 10 rats in each,and Wujiadan Recipe high(WH),Wujiadan Recipe medium(WM)and Wujiadan Recipe low dose group(WL)with 8 rats in each.Except the NG group,we removed the ovarian tissues of other six groups to establish the ovariectomized model.When the model is successfully replicated,we give the medicine to the rats in each group once a day.The TMC and WMC group were given the corresponding dose of Gengnian'an tablet suspension and Bujiale suspension by gavage respectively.The NG and MG group were given equal measure of normal saline.After 4 weeks,the rats were killed.We use ELISA to detecte the levels of PLC-β1,IP3,IP3R and Cam;and we use PCR to detecte the mRNA expression levels of PLC-βand IP3R1;and use WesternBlot to detecte the protein expression levels of Cam and GnRH in rat hypothalamus tissue.Results:The test results of PLC-β1,IP3,IP3R and Cam in the hypothalamus homogenate of the model group were significantly increased than the NG group(P<0.01).The results of PLC-β1 and IP3R1 and GnRHmRNA were significantly increased(P<0.01),and the expressions of GnRH and Cam protein were higher than NG(P<0.01).Wujiadan Recipe could reduce the results of PLC-β1,IP3,IP3R and Cam in hypothalamus of ovariectomized rats,and reduce the expression of PLC-β1 and IP3R1 and GnRH mRNA.Conclusion:Wujiadan Recipe down-regulates GnRH expression,which may be caused by acting on PLC-β1,IP3,IP3R,CaM in hypothalamus of ovariectomized rats,so as to coordinate the function of reproductive axis and improve the reproductive endocrine level of patients with menopausal syndrome.
文摘采用“二水法”湿法磷酸工艺对阿联酋某中品位磷矿进行了制酸评价研究,通过单因素实验得到了“二水法”湿法磷酸最适宜的生产控制条件,包括游离SO_(3)浓度、磷酸浓度、停留时间和液固比4个主要工艺参数。磷矿制酸系统在最适宜工艺参数控制下连续运行24 h,磷石膏滤饼采用二级逆流洗涤,磷矿中P_(2)O_(5)转化率可达到97.9%,回收率可达到96.9%,料浆的过滤强度约为10.5 t P_(2)O_(5)每平方米每天(TPD P_(2)O_(5)/m^(2))。
基金The subject was supported by the National Natural Science Foundation of China
文摘Alkylation of the ketimine 2 obtained from condensation of (+)-ketopinic acid and benzyla- mine with a variety of alkylating agents gives the products whose trends in diastereomeric excesses (1- 100% O. P.) appear to correlate with the structure and reactivity of electrophilic agents. Using excess n-butyl lithium and allylic or benzylic halides, β-alkylation occured.
