Isolated ruminal epithelia from caudal blind sacs of dairy goats were incubated with butyrate and insulin-like growth factor-1（IGF-1） at different concentrations. Proportions of ruminal epithelium in different phase...Isolated ruminal epithelia from caudal blind sacs of dairy goats were incubated with butyrate and insulin-like growth factor-1（IGF-1） at different concentrations. Proportions of ruminal epithelium in different phases of the cell division cycle were determined by flow cytometric analysis. The proportion of epithelial cells in S phase and G2-M phase（PS＆G2-M） increased significantly（P〈0.01） whereas the proportion of epithelial cells in G0-G1 phase（PG0-G1） decreased after incubation with IGF-1. PS＆G2-M decreased whereas PG0-G1 increased markedly（P〈0.01） after incubation with sodium butyrate. PS＆G2-M incubated with IGF-1 and butyrate sodium together increased more than that incubated with IGF-1 alone; PG0-G1, however, decreased significantly（P〈0.01）. Our results indicate that IGF-1 enhances whereas sodium butyrate inhibits the proliferation of rumen epithelial cells. Furthermore, butyrate and IGF-1, together, have a synergic effect on the proliferation of rumen epithelium.展开更多
回转支承广泛应用于旋挖钻机等需要在较大轴向力作用下实现周向自由转动的机械设备上。回转支承一旦发生故障会影响设备的正常工作,严重时还可能造成重大安全事故。文章以回转支承出现事故不能正常工作为出发点,采用故障树分析(Fault Tr...回转支承广泛应用于旋挖钻机等需要在较大轴向力作用下实现周向自由转动的机械设备上。回转支承一旦发生故障会影响设备的正常工作,严重时还可能造成重大安全事故。文章以回转支承出现事故不能正常工作为出发点,采用故障树分析(Fault Tree Analysis,FTA)方法对回转支承使用过程中各影响因素进行分析,发现影响工作可靠性的关键影响因素,结合实际运行/失效情况,对主要影响因素进行机理分析,并提出改进建议。本文研究对提高回转支承工作可靠性及使用寿命,保证设备安全稳定运行,提高社会及经济效益具有现实意义。展开更多
Fusarium head blight（FHB）,caused primarily by Fusarium graminearum,is a destructive disease of wheat throughout the world.However,the mechanisms of host resistance to FHB are still largely unclear.Deoxynivalenol（DO...Fusarium head blight（FHB）,caused primarily by Fusarium graminearum,is a destructive disease of wheat throughout the world.However,the mechanisms of host resistance to FHB are still largely unclear.Deoxynivalenol（DON）produced by F graminearum which enhances the pathogen to spread could be converted into inactive form D3 G by UDP-glycosyltransferases（UGTs）.A DON responsive UGT gene,designated as TaUGT4,was first cloned from wheat in this study.The putative open reading frame（ORF） of TaUGT4 was 1386 bp,encoding 461 amino acids protein.TaUGT4 was placed on chromosome 2D using a set of nulli-tetrasomic lines of wheat cultivar Chinese Spring（CS）.When fused with eGFP at C terminal,TaUGT4 was shown to localize in cytoplasm of the transformed tobacco cells.The transcriptional analysis revealed that TaUGT4 was strongly induced by F graminearum or DON in both of FHB-resistant cultivar Sumai 3 and susceptible cultivar Kenong 199,especially in Sumai 3 under DON treatment.Similar increase of TaUGT4 expression was observed in Sumai 3 and Kenong 199 in response to salicylic acid（SA） treatment.But interestingly,the transcripts level of TaUGT4 in Sumai 3 showed significantly higher than that in Kenong 199 after treated with methyl jasmonate（MeJA）.According to the expression patterns,TaUGT4 might lead to different effects between FHB-resistant genotype and susceptible genotype in the process against F graminearum inoculation.It had also been discussed in this paper that JA signaling pathway might play a significant role in the resistance against F.graminearum compared to SA signaling pathway.展开更多
基金supported by the National Natural Science Foundation of China(30960252)the China Agriculture Research System(CARS-37)
文摘Isolated ruminal epithelia from caudal blind sacs of dairy goats were incubated with butyrate and insulin-like growth factor-1（IGF-1） at different concentrations. Proportions of ruminal epithelium in different phases of the cell division cycle were determined by flow cytometric analysis. The proportion of epithelial cells in S phase and G2-M phase（PS＆G2-M） increased significantly（P〈0.01） whereas the proportion of epithelial cells in G0-G1 phase（PG0-G1） decreased after incubation with IGF-1. PS＆G2-M decreased whereas PG0-G1 increased markedly（P〈0.01） after incubation with sodium butyrate. PS＆G2-M incubated with IGF-1 and butyrate sodium together increased more than that incubated with IGF-1 alone; PG0-G1, however, decreased significantly（P〈0.01）. Our results indicate that IGF-1 enhances whereas sodium butyrate inhibits the proliferation of rumen epithelial cells. Furthermore, butyrate and IGF-1, together, have a synergic effect on the proliferation of rumen epithelium.
文摘回转支承广泛应用于旋挖钻机等需要在较大轴向力作用下实现周向自由转动的机械设备上。回转支承一旦发生故障会影响设备的正常工作,严重时还可能造成重大安全事故。文章以回转支承出现事故不能正常工作为出发点,采用故障树分析(Fault Tree Analysis,FTA)方法对回转支承使用过程中各影响因素进行分析,发现影响工作可靠性的关键影响因素,结合实际运行/失效情况,对主要影响因素进行机理分析,并提出改进建议。本文研究对提高回转支承工作可靠性及使用寿命,保证设备安全稳定运行,提高社会及经济效益具有现实意义。
基金supported by the National Natural Science Foundation of China(31071405 and 31171553)the National High-Tech R&D Program of China(863 Program,2011AA100102 and 2012AA101105)+3 种基金the Transgenic Special Item,China(2011ZX08002-004 and 2011ZX08009-003)the International Collaboration Program(948 Project,2013-S19)the Doctoral Fund of Ministry of Education of China(20133702120002)the Promotive Research Fund for Young and Middle-Aged Scientisits of Shandong Province,China(BS2013NY006)
文摘Fusarium head blight（FHB）,caused primarily by Fusarium graminearum,is a destructive disease of wheat throughout the world.However,the mechanisms of host resistance to FHB are still largely unclear.Deoxynivalenol（DON）produced by F graminearum which enhances the pathogen to spread could be converted into inactive form D3 G by UDP-glycosyltransferases（UGTs）.A DON responsive UGT gene,designated as TaUGT4,was first cloned from wheat in this study.The putative open reading frame（ORF） of TaUGT4 was 1386 bp,encoding 461 amino acids protein.TaUGT4 was placed on chromosome 2D using a set of nulli-tetrasomic lines of wheat cultivar Chinese Spring（CS）.When fused with eGFP at C terminal,TaUGT4 was shown to localize in cytoplasm of the transformed tobacco cells.The transcriptional analysis revealed that TaUGT4 was strongly induced by F graminearum or DON in both of FHB-resistant cultivar Sumai 3 and susceptible cultivar Kenong 199,especially in Sumai 3 under DON treatment.Similar increase of TaUGT4 expression was observed in Sumai 3 and Kenong 199 in response to salicylic acid（SA） treatment.But interestingly,the transcripts level of TaUGT4 in Sumai 3 showed significantly higher than that in Kenong 199 after treated with methyl jasmonate（MeJA）.According to the expression patterns,TaUGT4 might lead to different effects between FHB-resistant genotype and susceptible genotype in the process against F graminearum inoculation.It had also been discussed in this paper that JA signaling pathway might play a significant role in the resistance against F.graminearum compared to SA signaling pathway.
