加速康复外科(enhanced recovery after surgery,ERAS)旨在通过优化围手术期处理,减轻手术应激,缩短患者术后恢复时间,现已广泛应用于结直肠外科、骨科、泌尿外科等领域,并逐渐被妇科医生所重视。已有研究表明,无论在妇科良性疾病还是...加速康复外科(enhanced recovery after surgery,ERAS)旨在通过优化围手术期处理,减轻手术应激,缩短患者术后恢复时间,现已广泛应用于结直肠外科、骨科、泌尿外科等领域,并逐渐被妇科医生所重视。已有研究表明,无论在妇科良性疾病还是恶性肿瘤手术中,ERAS均能够减轻患者术后疼痛、降低术后恶心呕吐发生率、促进术后肠道功能恢复,缩短住院日及降低住院费用,提高患者满意度,同时不增加术后并发症及再次住院发生率。ERAS的实施需要多学科紧密协作,同时需要设计严谨科学的前瞻性研究提供高质量的证据支持,推动其不断发展和完善,使患者实际获益。展开更多
加速康复外科(enhanced recovery after surgery,ERAS)是以循证医学为基础,以减少手术创伤与应激为目的,通过优化围手术期临床路径,促进患者术后快速恢复。其基本原则包括:术前宣教、取消常规肠道准备、术前2 h摄入含糖饮料、多模式镇...加速康复外科(enhanced recovery after surgery,ERAS)是以循证医学为基础,以减少手术创伤与应激为目的,通过优化围手术期临床路径,促进患者术后快速恢复。其基本原则包括:术前宣教、取消常规肠道准备、术前2 h摄入含糖饮料、多模式镇痛、术中体温监测、优化液体管理、避免放置引流、术后早期进食及下床活动等。ERAS的成功实施需要多学科密切合作,制定可供临床遵循的规范与流程,但同时需充分结合各医疗中心实际条件与患者具体情况,在标准化的同时做到个体化、最优化,使患者实际获益。展开更多
Background Stathmin was identified as an endometriosis-related protein by comparative proteomics in our previous study.As a microtubule-destablizing factor, stathmin was shown to participate in the relay and integrati...Background Stathmin was identified as an endometriosis-related protein by comparative proteomics in our previous study.As a microtubule-destablizing factor, stathmin was shown to participate in the relay and integration of diverse intracellular signaling pathways involved in cell proliferation, differentiation, and many other cellular activities.To investigate whether stathmin is involved in the pathogenesis of endometriosis, we examined the expression of stathmin in eutopic endometrium of women with or without endometriosis.Methods Eutopic endometrium samples were collected from thirty-six patients who were diagnosed as endometriosis and the nineteen age-matched patients who were confirmed to be free of endometriosis surgically and histologically.The expression of stathmin mRNA was detected by real-time PCR, and its protein was detected by Western blotting and immunohistochemistry.Results Stathmin was overexpressed in eutopic endometrium of women with endometriosis detected by real-time PCR in mRNA levels and by Western blotting in protein levels, without significant difference between proliferative and 0secretory phase.Immunohistochemistry showed that stathmin protein was localized in both endometrial glandular and stromal cells throughout the menstrual cycle.Conclusions Stathmin is overexpressed in endometrium of patients with endometriosis and may play a role in the pathogenesis of endometriosis.展开更多
文摘加速康复外科(enhanced recovery after surgery,ERAS)旨在通过优化围手术期处理,减轻手术应激,缩短患者术后恢复时间,现已广泛应用于结直肠外科、骨科、泌尿外科等领域,并逐渐被妇科医生所重视。已有研究表明,无论在妇科良性疾病还是恶性肿瘤手术中,ERAS均能够减轻患者术后疼痛、降低术后恶心呕吐发生率、促进术后肠道功能恢复,缩短住院日及降低住院费用,提高患者满意度,同时不增加术后并发症及再次住院发生率。ERAS的实施需要多学科紧密协作,同时需要设计严谨科学的前瞻性研究提供高质量的证据支持,推动其不断发展和完善,使患者实际获益。
文摘加速康复外科(enhanced recovery after surgery,ERAS)是以循证医学为基础,以减少手术创伤与应激为目的,通过优化围手术期临床路径,促进患者术后快速恢复。其基本原则包括:术前宣教、取消常规肠道准备、术前2 h摄入含糖饮料、多模式镇痛、术中体温监测、优化液体管理、避免放置引流、术后早期进食及下床活动等。ERAS的成功实施需要多学科密切合作,制定可供临床遵循的规范与流程,但同时需充分结合各医疗中心实际条件与患者具体情况,在标准化的同时做到个体化、最优化,使患者实际获益。
基金This work was supported by the grants from National Natural Science Foundation of China （No.30600669） and the Young Scientists from Peking Union Medical College Hospital （No.20054499）.
文摘Background Stathmin was identified as an endometriosis-related protein by comparative proteomics in our previous study.As a microtubule-destablizing factor, stathmin was shown to participate in the relay and integration of diverse intracellular signaling pathways involved in cell proliferation, differentiation, and many other cellular activities.To investigate whether stathmin is involved in the pathogenesis of endometriosis, we examined the expression of stathmin in eutopic endometrium of women with or without endometriosis.Methods Eutopic endometrium samples were collected from thirty-six patients who were diagnosed as endometriosis and the nineteen age-matched patients who were confirmed to be free of endometriosis surgically and histologically.The expression of stathmin mRNA was detected by real-time PCR, and its protein was detected by Western blotting and immunohistochemistry.Results Stathmin was overexpressed in eutopic endometrium of women with endometriosis detected by real-time PCR in mRNA levels and by Western blotting in protein levels, without significant difference between proliferative and 0secretory phase.Immunohistochemistry showed that stathmin protein was localized in both endometrial glandular and stromal cells throughout the menstrual cycle.Conclusions Stathmin is overexpressed in endometrium of patients with endometriosis and may play a role in the pathogenesis of endometriosis.
