The variable domain of heavy chain (VH) and light chain (VL) genes of anti-CD20 monoclonal antibody HL47 were cloned from anti-CD20 ScFv expression vector pCANBTEcd20 by PCR and ligated into vector pYZF to construct c...The variable domain of heavy chain (VH) and light chain (VL) genes of anti-CD20 monoclonal antibody HL47 were cloned from anti-CD20 ScFv expression vector pCANBTEcd20 by PCR and ligated into vector pYZF to construct chimeric anti-CD20 Fab’ fragment expression vector pTZFcd20. Chimeric anti-CD20 Fab’ fragment was expressed in E. coli 16C9 and purified by protein G affinity chromatography. Competitive inhibition assay showed that anti-CD20 Fab’ fragment inhibited binding of HI47 to CD20 on the surface of Daudi cells. Results from MTT assay indicated that chimeric anti-CD20 Fab’fragment inhibited the proliferation of Daudi cells, IC50=69 ug/mL. Affinity of chimeric anti-CD20 Fab’ fragment was determined, Ka was about 8.9×108 (mol/L)-1.展开更多
The binding of recombinant human macrophage colony-stimulating factor (M-CSF) soluble receptor (rh-M-CSF-sR) to membrane-bound macrophage colony stimulating factor (m-M-CSF) and the internalization and recycling of rh...The binding of recombinant human macrophage colony-stimulating factor (M-CSF) soluble receptor (rh-M-CSF-sR) to membrane-bound macrophage colony stimulating factor (m-M-CSF) and the internalization and recycling of rh-M-CSF-sR/m-M-CSF complexes mediated by m-M-CSF were studied with a model of J6-1 cell line. The results indicated that m-M-CSF bound rh-M-CSF-sR with high affinity (Kd= 1.78×10 12 mol/L) and mediated a temperature- and energy-dependent internalization of rh-M-CSF-sR, and that internalized rh-M-CSF-sR could return to the cell surface in an m-M-CSF-bound state, suggesting that m-M-CSF may have a capability to mediate the internalization and recycling of rh-M-CSF-sR/m-M-CSF complexes. In addition, the half-lives of cell-associated M-CSF and its receptor of stimulated normal human cord blood mononuclear cells (CBMCs) and 4 leukemic cell lines were measured by indirect immunoflu-orescence and flow cytometry. The results showed that the half-lives of the various kinds of M-CSF isoforms and展开更多
The outbreak of coronavirus disease 2019(COVID-19)has been spreading rapidly in China and the Chinese government took a series of policies to control the epidemic.Therefore,it will be helpful to predict the tendency o...The outbreak of coronavirus disease 2019(COVID-19)has been spreading rapidly in China and the Chinese government took a series of policies to control the epidemic.Therefore,it will be helpful to predict the tendency of the epidemic and analyze the influence of official policies.Existing models for prediction,such as cabin models and individual-based models,are either oversimplified or too meticulous,and the influence of the epidemic was studied much more than that of official policies.To predict the epidemic tendency,we consider four groups of people,and establish a propagation dynamics model.We also create a negative feedback to quantify the public vigilance to the epidemic.We evaluate the tendency of epidemic in Hubei and China except Hubei separately to predict the situation of the whole country.Experiments show that the epidemic will terminate around 17 March 2020 and the final number of cumulative infections will be about 78191(prediction interval,74872 to 82474).By changing the parameters of the model accordingly,we demonstrate the control effect of the policies of the government on the epidemic situation,which can reduce about 68%possible infections.At the same time,we use the capital asset pricing model with dummy variable to evaluate the effects of the epidemic and official policies on the revenue of multiple industries.展开更多
基金This work was supported by the State "863" High-Tech Project (Grant No. 102-09-03-03).
文摘The variable domain of heavy chain (VH) and light chain (VL) genes of anti-CD20 monoclonal antibody HL47 were cloned from anti-CD20 ScFv expression vector pCANBTEcd20 by PCR and ligated into vector pYZF to construct chimeric anti-CD20 Fab’ fragment expression vector pTZFcd20. Chimeric anti-CD20 Fab’ fragment was expressed in E. coli 16C9 and purified by protein G affinity chromatography. Competitive inhibition assay showed that anti-CD20 Fab’ fragment inhibited binding of HI47 to CD20 on the surface of Daudi cells. Results from MTT assay indicated that chimeric anti-CD20 Fab’fragment inhibited the proliferation of Daudi cells, IC50=69 ug/mL. Affinity of chimeric anti-CD20 Fab’ fragment was determined, Ka was about 8.9×108 (mol/L)-1.
文摘The binding of recombinant human macrophage colony-stimulating factor (M-CSF) soluble receptor (rh-M-CSF-sR) to membrane-bound macrophage colony stimulating factor (m-M-CSF) and the internalization and recycling of rh-M-CSF-sR/m-M-CSF complexes mediated by m-M-CSF were studied with a model of J6-1 cell line. The results indicated that m-M-CSF bound rh-M-CSF-sR with high affinity (Kd= 1.78×10 12 mol/L) and mediated a temperature- and energy-dependent internalization of rh-M-CSF-sR, and that internalized rh-M-CSF-sR could return to the cell surface in an m-M-CSF-bound state, suggesting that m-M-CSF may have a capability to mediate the internalization and recycling of rh-M-CSF-sR/m-M-CSF complexes. In addition, the half-lives of cell-associated M-CSF and its receptor of stimulated normal human cord blood mononuclear cells (CBMCs) and 4 leukemic cell lines were measured by indirect immunoflu-orescence and flow cytometry. The results showed that the half-lives of the various kinds of M-CSF isoforms and
基金the National Key Research and Development Program of China(No.2018YFB1004700)the National Natural Science Foundation of China(Nos.61872238 and 61972254)+1 种基金the Shanghai Science and Technology Fund(No.17510740200)the CCFHuawei Database System Innovation Research Plan(No.CCF-Huawei DBIR2019002A)。
文摘The outbreak of coronavirus disease 2019(COVID-19)has been spreading rapidly in China and the Chinese government took a series of policies to control the epidemic.Therefore,it will be helpful to predict the tendency of the epidemic and analyze the influence of official policies.Existing models for prediction,such as cabin models and individual-based models,are either oversimplified or too meticulous,and the influence of the epidemic was studied much more than that of official policies.To predict the epidemic tendency,we consider four groups of people,and establish a propagation dynamics model.We also create a negative feedback to quantify the public vigilance to the epidemic.We evaluate the tendency of epidemic in Hubei and China except Hubei separately to predict the situation of the whole country.Experiments show that the epidemic will terminate around 17 March 2020 and the final number of cumulative infections will be about 78191(prediction interval,74872 to 82474).By changing the parameters of the model accordingly,we demonstrate the control effect of the policies of the government on the epidemic situation,which can reduce about 68%possible infections.At the same time,we use the capital asset pricing model with dummy variable to evaluate the effects of the epidemic and official policies on the revenue of multiple industries.
