目的研究高迁移率族蛋白-1(high-mobility group box-1,HMGB 1)和晚期糖基化终末产物受体(receptor for advanced glycation end products,RAGE)在多囊卵巢综合征(polycystic ovary syndrome,PCOS)大鼠模型卵巢组织中的表达。方法采用...目的研究高迁移率族蛋白-1(high-mobility group box-1,HMGB 1)和晚期糖基化终末产物受体(receptor for advanced glycation end products,RAGE)在多囊卵巢综合征(polycystic ovary syndrome,PCOS)大鼠模型卵巢组织中的表达。方法采用脱氢表雄酮联合高脂饮食造模法建立PCOS大鼠模型,造模成功后用Real-time PCR和Western-Blot检测HMGB 1和RAGE的表达,用免疫组织化学染色检测HMGB 1的表达。结果 HMGB 1 mRNA、RAGE mRNA、HMGB 1蛋白、RAGE蛋白在研究组的表达量均高于对照组,差异均有统计学意义(P<0.05)。结论研究组大鼠卵巢组织中的HMGB1和RAGE的表达量均明显高于对照组,提示HMGB 1和RAGE可能在PCOS的发生发展中有一定作用。展开更多
Background In patients suffering from acute pancreatitis, the pathogenesis is not completely understood, and several recent studies in vitro suggested that heat shock proteins might play an important role in cell sign...Background In patients suffering from acute pancreatitis, the pathogenesis is not completely understood, and several recent studies in vitro suggested that heat shock proteins might play an important role in cell signaling. To investigate the possible role of extracellular heat shock protein 70 （Hsp70） in pancreatitis, toll-like receptor-4 （TLR4）-deficient and wild-type mice were administered with exogenous Hsp70 during the course of cerulein-induced pancreatitis （CIP）. Methods Acute pancreatitis was induced by 5 intraperitoneal injections of cerulein at hourly intervals, and then treated with recombinant Hsp70 through the caudal vein 4 hours after the start of cerulein injections. Subsequently serum amylase and serum cytokines levels were detected. Histologic alteration of the pancreas was evaluated. Tumor necrosis factor alpha （TNF-α） concentrations and myeloperoxidase （MPO） activity in both pancreas and lungs were analyzed. The nuclear factor kappa B （NF-κB） activation in pancreatic tissue was measured using a sensitive RelA enzyme-linked immunosorbent assay. Results Treatment with recombinant Hsp70 to wild-type mice in CIP resulted in significant aggravation of inflammation in pancreas, elevated levels of serum cytokines, up-regulation of pulmonary MPO activity and increase of lung tissues TNF-α concentrations. In contrast, treatment with Hsp70 to TLR4-deficient mice had little effect on serum cytokines levels pancreatic inflammation, pulmonary MPO activity and TNF-α concentrations. Conclusions The results suggest that extracellular Hsp70 might induce systemic inflammatory response syndrome （SlRS）-Iike response in vivo and TLR4 might be involved in the Hsp70-mediated activation of inflammatory reaction in the progression of CIP without infection.展开更多
文摘试验旨在研究不同Toll样受体(Toll-like receptor,TLR)在鸭不同组织中的表达情况,选取300日龄雄性金定鸭10只,解剖后采集其血液及脾脏、肝脏、睾丸、肺脏、下丘脑、垂体、皮肤、腿肌、心脏、肾脏、胸肌、盲肠、小肠、胸腺,采用Primer Premier 5.0软件设计特异性引物,并用实时荧光定量PCR法检测TLR1、TLR2、TLR4、TLR5在不同组织中的相对表达情况。结果显示,各基因扩增产物的熔解曲线均有一特异性的单峰,无其他杂峰,说明引物的特异性较强,可以准确定量。4种目的基因与内参基因的扩增效率在101.4%~105.0%之间,均接近100%,相关系数(R2)为0.98~1.000。TLR1、TLR2、TLR4、TLR5 4种TLRs在金定鸭不同组织和血液中均有表达,但每种TLR受体在各组织中表达水平略有差异,其中TLR1在下丘脑中表达量最低,在胸肌中最高;TLR2在小肠中的表达量最低,在肺脏中最高;TLR4、TLR5在睾丸中的表达量最低,在皮肤中最高。以上结果说明,鸭TLRs在多种组织中能够广泛表达,本试验结果可为TLRs在鸭源感染过程中的作用机理研究提供科学依据。
文摘为了解近年来山东地区猪繁殖与呼吸综合征病毒(PRRSV)的流行及分子遗传变异情况。对2018—2019年山东不同地区493份疑似PRRSV样品进行RT-PCR检测,并对34份PRRSV阳性样品进行ORF 5基因核苷酸及GP5蛋白氨基酸序列分析比较。结果显示,2018年和2019年送检样品中PRRSV抗原阳性率分别为20.79%和24.09%;同源性对比及遗传进化分析,34株PRRSV主要属于谱系1和谱系8,且2018年和2019年均为谱系1中的PRRSV检出率最高,分别为50.00%和59.10%;氨基酸分析显示,34株PRRSV主要以点突变为主,未见插入突变,与不同谱系代表毒株相比,诱饵表位、中和表位、潜在毒力位点及N-糖基化位点均存在不同程度的变异。结果表明,NADC 30 like PRRSV可能已成为山东地区优势野毒毒株,且仍在不断发生变异。
基金This study was supported by grants from the National Natural Science Foundation of China （No. 30400434 and No. 30571811） and China Medical Board （No. 96636 ）.
文摘Background In patients suffering from acute pancreatitis, the pathogenesis is not completely understood, and several recent studies in vitro suggested that heat shock proteins might play an important role in cell signaling. To investigate the possible role of extracellular heat shock protein 70 （Hsp70） in pancreatitis, toll-like receptor-4 （TLR4）-deficient and wild-type mice were administered with exogenous Hsp70 during the course of cerulein-induced pancreatitis （CIP）. Methods Acute pancreatitis was induced by 5 intraperitoneal injections of cerulein at hourly intervals, and then treated with recombinant Hsp70 through the caudal vein 4 hours after the start of cerulein injections. Subsequently serum amylase and serum cytokines levels were detected. Histologic alteration of the pancreas was evaluated. Tumor necrosis factor alpha （TNF-α） concentrations and myeloperoxidase （MPO） activity in both pancreas and lungs were analyzed. The nuclear factor kappa B （NF-κB） activation in pancreatic tissue was measured using a sensitive RelA enzyme-linked immunosorbent assay. Results Treatment with recombinant Hsp70 to wild-type mice in CIP resulted in significant aggravation of inflammation in pancreas, elevated levels of serum cytokines, up-regulation of pulmonary MPO activity and increase of lung tissues TNF-α concentrations. In contrast, treatment with Hsp70 to TLR4-deficient mice had little effect on serum cytokines levels pancreatic inflammation, pulmonary MPO activity and TNF-α concentrations. Conclusions The results suggest that extracellular Hsp70 might induce systemic inflammatory response syndrome （SlRS）-Iike response in vivo and TLR4 might be involved in the Hsp70-mediated activation of inflammatory reaction in the progression of CIP without infection.
