Perfluorooctanoic acid(PFOA)is a widespread synthetic persistent organic pollutant that may enrich along the food chain and affect the growth,development,reproduction,and lipid metabolism of aquatic organisms,particul...Perfluorooctanoic acid(PFOA)is a widespread synthetic persistent organic pollutant that may enrich along the food chain and affect the growth,development,reproduction,and lipid metabolism of aquatic organisms,particularly the benthic organisms.How-ever,the toxic effects of PFOA on the half-smooth tongue sole Cynoglossus semilaevis,a commercial benthic fish in China,have rarely been reported.Because juvenile fish are sensitive to environmental pollutants,in the present study,histological assessment and tran-scriptome sequencing were performed to determine the short-term impact of PFOA on juvenile half-smooth tongue soles.Histologi-cal analysis showed that PFOA exposure caused hepatocyte rupture,intestinal villi breakage,increased goblet cell count,and brain ab-normal.Transcriptome results showed that some interesting signaling pathways,such as glycolysis/gluconeogenesis,PPAR signaling pathway and GABAergic synapse signaling pathway,were enriched after PFOA exposure.In addition,some metabolic,immune and neural genes were differentially expressed,which including ependymin,hbb1-like and gad 1,and they were up-regulated after 14 days of exposure.Transcriptome results also indicated that half-smooth tongue sole might improve energy metabolism in response to PFOA toxicity after 7 days of exposure.These findings provide a basis for studying the ecological effects of PFOA on marine benthic fishes.展开更多
目的基于网络药理学探析疏风解毒胶囊治疗新型冠状病毒肺炎(corona virus disease,COVID-19)的潜在分子机制。方法通过检索中药药理学分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCM...目的基于网络药理学探析疏风解毒胶囊治疗新型冠状病毒肺炎(corona virus disease,COVID-19)的潜在分子机制。方法通过检索中药药理学分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)数据库查找筛选疏风解毒胶囊的化学活性成分,并运用PubChem数据库预测疏风解毒胶囊人类靶蛋白。利用GeneCards数据库获取并筛选COVID-19人类基因,数据导入生物通路分析(Ingenuity Pathway Analysis,IPA)软件,比较疏风解毒胶囊靶蛋白和COVID-19人类基因之间相同的分子网络、生物学通路及位点,预测药物作用机制。结果通过GeneCards数据库获取COVID-19相关基因546个,TCMSP数据库筛选得到101个类药性良好的活性成分,PubChem数据库对应预测出靶蛋白102个。经过IPA分析发现,疏风解毒胶囊与COVID-19共有的生物学通路有331条,多数集中在炎症、免疫以及细胞增殖、凋亡和迁移的相关通路。作用于趋化因子受体1(chemokine receptor 1,CXCR1)、表皮生长因子受体(epidermal growth factor receptor,EGFR)、基质金属蛋白酶-2(matrix metalloproteinase 2,MMP2)、基质金属蛋白酶-9(matrix metalloproteinase 9,MMP9)、磷脂酰肌醇3-激酶(phosphatidylinositol 3-kinase,PI3K)及蛋白激酶B(protein kinase B,PKB/AKT)等位点。结论疏风解毒胶囊可能通过与炎症、免疫反应以及细胞增殖、凋亡和迁移等相关生物通路治疗COVID-19,其作用机制可能与趋化免疫细胞、激活多种炎性因子的表达以及抑制病毒等方面有关。展开更多
基金supported by the National Key R&D Program(No.2018YFD0900301-03)the MNR Key Laboratory of Marine Eco-Environmental Science and Technology,China(No.MEEST-2021-04).
文摘Perfluorooctanoic acid(PFOA)is a widespread synthetic persistent organic pollutant that may enrich along the food chain and affect the growth,development,reproduction,and lipid metabolism of aquatic organisms,particularly the benthic organisms.How-ever,the toxic effects of PFOA on the half-smooth tongue sole Cynoglossus semilaevis,a commercial benthic fish in China,have rarely been reported.Because juvenile fish are sensitive to environmental pollutants,in the present study,histological assessment and tran-scriptome sequencing were performed to determine the short-term impact of PFOA on juvenile half-smooth tongue soles.Histologi-cal analysis showed that PFOA exposure caused hepatocyte rupture,intestinal villi breakage,increased goblet cell count,and brain ab-normal.Transcriptome results showed that some interesting signaling pathways,such as glycolysis/gluconeogenesis,PPAR signaling pathway and GABAergic synapse signaling pathway,were enriched after PFOA exposure.In addition,some metabolic,immune and neural genes were differentially expressed,which including ependymin,hbb1-like and gad 1,and they were up-regulated after 14 days of exposure.Transcriptome results also indicated that half-smooth tongue sole might improve energy metabolism in response to PFOA toxicity after 7 days of exposure.These findings provide a basis for studying the ecological effects of PFOA on marine benthic fishes.
