目的探究内蒙古通辽地区牛肉中挥发性风味物质及特征风味物质,以牛肉的前腿、后腿和背脊为研究对象,对其挥发性风味物质进行测定分析。方法利用固相微萃取结合气相色谱-质谱法(solid phase microextraction coupled with gas chromatogr...目的探究内蒙古通辽地区牛肉中挥发性风味物质及特征风味物质,以牛肉的前腿、后腿和背脊为研究对象,对其挥发性风味物质进行测定分析。方法利用固相微萃取结合气相色谱-质谱法(solid phase microextraction coupled with gas chromatography-mass spectrometry,SPME-GC-MS)及AOC-6000自动进样器对牛肉3个部位共12个样本进行分析。结果共鉴定出117种挥发性成分,包括酮类、醇类、醛类、酸类、酯类、烷烃类和其他类化合物。利用偏最小二乘判别分析及聚类热图分析,较好的识别3种部位之间的关系,结合相对气味活度值(relative odor activity value,ROAV)初步得出影响通辽牛肉的9种风味化合物,分别为十六醛、己醛、庚醛、辛醛、壬醛、(E)-2-壬烯醛、癸醛、(E)-2-癸烯醛和1-辛烯-3-醇。结论本研究探讨了通辽牛肉3个部位中挥发性风味物质的相互关系,初步确定牛肉的关键性风味化合物,以期为提升牛肉风味及其品质评价提供科学依据。展开更多
Chitooligosaccharide-zinc(COS·Zn)is a powerful anti-oxidant and anti-aging scavenger,whose anti-oxidative ability immensely exceeds vitamin C.Therefore,this study was aimed to investigate the protective effects o...Chitooligosaccharide-zinc(COS·Zn)is a powerful anti-oxidant and anti-aging scavenger,whose anti-oxidative ability immensely exceeds vitamin C.Therefore,this study was aimed to investigate the protective effects of COS·Zn against premature ovarian failure(POF)and potential mechanisms.Female KM adult mice were divided into the following groups:a treatment group(150 mg·kg^(−1)·d^(−1) COS·Zn),a treatment group(300 mg·kg^(−1)·d^(−1) COS·Zn),a prevention group,two control groups and two CY/BUS groups.COS·Zn(150,300 mg·kg^(−1)·d^(−1))and COS·Zn(300 mg·kg^(−1)·d^(−1))were therapeutically and preventatively administered to POF mice in the treatment and prevention studies,respectively.All the groups were administered for 21 days.Fewer primary and secondary follicles were observed in the COS·Zn-treated groups(including the treatment and prevention groups)than those of the control groups.Meanwhile,the ovarian index and the levels of FSH and LH notably increased in the treatment and prevention groups compared with those in the CY/BUS group.The levels of MVH,OCT4 and PCNA in the treatment group(300·kg^(−1)·d^(−1) COS·Zn)and MVH in the prevention group remarkably increased compared with those in the CY/BUS groups.Meanwhile,the levels of P53 and P16 protein were down-regulated in the treatment and prevention groups compared with those in the CY/BUS groups.Additionally,the amounts of Sestrin2(SESN2)and SOD2 protein were obviously higher in the treatment group(150 mg·kg^(−1)·d^(−1) COS·Zn)than those in the CY/BUS groups.Similarly,the amounts of NRF2 and SESN2 protein were up-regulated in the prevention group.Besides,an increased GSH level was observed in the two treatment groups,compared with that in the CY/BUS groups,and the same trend was also present in the prevention group.Taken together,COS·Zn improves the ovarian and follicular development through regulating the SESN2/NRF2 signaling pathway.These results suggest the role of COS·Zn as a novel agent for the treatment and prevention of POF.展开更多
基金supported by the Natural Science Foundation of Jiangxi,China(No.20192BAB215009)the National Natural Science Foundation of China(Nos.31460307,81671455 and 81771583)+1 种基金the Key Project of Jiangxi Province Natural Science Youth Fund(No.20202ACB216003)the Young Teacher Research and Training Foundation of Nanchang University Medical Department(No.PY201814).
文摘Chitooligosaccharide-zinc(COS·Zn)is a powerful anti-oxidant and anti-aging scavenger,whose anti-oxidative ability immensely exceeds vitamin C.Therefore,this study was aimed to investigate the protective effects of COS·Zn against premature ovarian failure(POF)and potential mechanisms.Female KM adult mice were divided into the following groups:a treatment group(150 mg·kg^(−1)·d^(−1) COS·Zn),a treatment group(300 mg·kg^(−1)·d^(−1) COS·Zn),a prevention group,two control groups and two CY/BUS groups.COS·Zn(150,300 mg·kg^(−1)·d^(−1))and COS·Zn(300 mg·kg^(−1)·d^(−1))were therapeutically and preventatively administered to POF mice in the treatment and prevention studies,respectively.All the groups were administered for 21 days.Fewer primary and secondary follicles were observed in the COS·Zn-treated groups(including the treatment and prevention groups)than those of the control groups.Meanwhile,the ovarian index and the levels of FSH and LH notably increased in the treatment and prevention groups compared with those in the CY/BUS group.The levels of MVH,OCT4 and PCNA in the treatment group(300·kg^(−1)·d^(−1) COS·Zn)and MVH in the prevention group remarkably increased compared with those in the CY/BUS groups.Meanwhile,the levels of P53 and P16 protein were down-regulated in the treatment and prevention groups compared with those in the CY/BUS groups.Additionally,the amounts of Sestrin2(SESN2)and SOD2 protein were obviously higher in the treatment group(150 mg·kg^(−1)·d^(−1) COS·Zn)than those in the CY/BUS groups.Similarly,the amounts of NRF2 and SESN2 protein were up-regulated in the prevention group.Besides,an increased GSH level was observed in the two treatment groups,compared with that in the CY/BUS groups,and the same trend was also present in the prevention group.Taken together,COS·Zn improves the ovarian and follicular development through regulating the SESN2/NRF2 signaling pathway.These results suggest the role of COS·Zn as a novel agent for the treatment and prevention of POF.
