In this work,we prepared a material with magnetic nanoparticles(Fe_(3)O_(4))as core,layered double hydroxides(LDHs)as affinity shell,and cerium dioxide(CeO_(2))as functional molecules(denoted as Fe_(3)O_(4)@LDH-CeO_(2...In this work,we prepared a material with magnetic nanoparticles(Fe_(3)O_(4))as core,layered double hydroxides(LDHs)as affinity shell,and cerium dioxide(CeO_(2))as functional molecules(denoted as Fe_(3)O_(4)@LDH-CeO_(2)).On the basis of combined immobilized metal ion affinity chromatography(IMAC)and metal oxide affinity chromatography(MOAC),Fe_(3)O_(4)@LDH-CeO_(2) was used to enrich phosphopeptides with high efficiency.The material exhibited high selectivity(α-casein:β-casein:BSA=1:1:5000,mass ratio),high recovery(95.87%),and good reusability of 10 times adsorption-desorption experiments.The feasibility of Fe_(3)O_(4)@LDH-CeO_(2) was further investigated by extracting phosphopeptides from biological samples(nonfat milk,serum,saliva,and A549 cell lysate).展开更多
基金National Natural Science Foundation of China(Nos.22004073 and 21961024)Natural Science Foundation of Inner Mongolia,China(No.2020BS02010)+2 种基金Talent Research Support Funds from Government-Sponsored Institution of Inner Mongolia,China(No.RCQD19002)Inner Mongolia Minzu University Doctoral Research Startup Fund Project,China(No.BS516)Incentive Funding from Nano Innovation Institute(NII)of Inner Mongolia Minzu University,China.
文摘In this work,we prepared a material with magnetic nanoparticles(Fe_(3)O_(4))as core,layered double hydroxides(LDHs)as affinity shell,and cerium dioxide(CeO_(2))as functional molecules(denoted as Fe_(3)O_(4)@LDH-CeO_(2)).On the basis of combined immobilized metal ion affinity chromatography(IMAC)and metal oxide affinity chromatography(MOAC),Fe_(3)O_(4)@LDH-CeO_(2) was used to enrich phosphopeptides with high efficiency.The material exhibited high selectivity(α-casein:β-casein:BSA=1:1:5000,mass ratio),high recovery(95.87%),and good reusability of 10 times adsorption-desorption experiments.The feasibility of Fe_(3)O_(4)@LDH-CeO_(2) was further investigated by extracting phosphopeptides from biological samples(nonfat milk,serum,saliva,and A549 cell lysate).
