旨在建立崂山奶山羊脂肪间充质干细胞系并对其进行初步鉴定。采集崂山奶山羊脂肪组织,在Ⅰ型胶原酶的分解消化作用下,将脂肪组织中的单核细胞进行分离并扩增培养;测定其生长曲线,利用Real time RT-PCR技术对其表达的干细胞因子进行鉴定...旨在建立崂山奶山羊脂肪间充质干细胞系并对其进行初步鉴定。采集崂山奶山羊脂肪组织,在Ⅰ型胶原酶的分解消化作用下,将脂肪组织中的单核细胞进行分离并扩增培养;测定其生长曲线,利用Real time RT-PCR技术对其表达的干细胞因子进行鉴定;将传至第3代的脂肪间充质干细胞进行成骨诱导分化,利用茜素红染色进行鉴定。结果显示,崂山奶山羊脂肪间充质干细胞形态为长梭形、星形等成纤维细胞样细胞形态,但比成纤维细胞饱满,第3代细胞生长至3 d左右时细胞进入指数生长期,生长至6~7 d时进入平台期;经成骨诱导分化后,经茜素红染色可见骨结节被染成深红色。综上提示,崂山奶山羊脂肪间充质干细胞具有诱导分化潜能。展开更多
[ Objective] To open an exit for breeding high-fecundity Small Tail Han sheep according to genotypes. [ Method] By the PCR-RFLP, the polymorphism of ovine BMPR-IB gene was detected in Small Tail Han sheep of basic pop...[ Objective] To open an exit for breeding high-fecundity Small Tail Han sheep according to genotypes. [ Method] By the PCR-RFLP, the polymorphism of ovine BMPR-IB gene was detected in Small Tail Han sheep of basic population and breeding population. And its relationship with litter size was analyzed. [ Result] In the basic population, the frequencies of B ＋ and BB genotypes were 77.78% and 14.81%, respectively, and their litter size per parity was 1.77 and 2.40, respectively. In the breeding population, the frequencies of B ＋ and BB genotypes were 51.79% and 40.18%, respectively, and their litter size per parity was 2.54 and 3.02, respectively. [ Conclusion] The ovine BMPR-IB gene can be used as a molecular Qenetic marker for fecundity traits to establish high-fecundity Small Tail Han sheep.展开更多
文摘旨在建立崂山奶山羊脂肪间充质干细胞系并对其进行初步鉴定。采集崂山奶山羊脂肪组织,在Ⅰ型胶原酶的分解消化作用下,将脂肪组织中的单核细胞进行分离并扩增培养;测定其生长曲线,利用Real time RT-PCR技术对其表达的干细胞因子进行鉴定;将传至第3代的脂肪间充质干细胞进行成骨诱导分化,利用茜素红染色进行鉴定。结果显示,崂山奶山羊脂肪间充质干细胞形态为长梭形、星形等成纤维细胞样细胞形态,但比成纤维细胞饱满,第3代细胞生长至3 d左右时细胞进入指数生长期,生长至6~7 d时进入平台期;经成骨诱导分化后,经茜素红染色可见骨结节被染成深红色。综上提示,崂山奶山羊脂肪间充质干细胞具有诱导分化潜能。
基金supported by Agricultural Stock Breeding Project of Shandong Province(Breeding of New Breeds of Excellent Local Mutton Sheep)
文摘[ Objective] To open an exit for breeding high-fecundity Small Tail Han sheep according to genotypes. [ Method] By the PCR-RFLP, the polymorphism of ovine BMPR-IB gene was detected in Small Tail Han sheep of basic population and breeding population. And its relationship with litter size was analyzed. [ Result] In the basic population, the frequencies of B ＋ and BB genotypes were 77.78% and 14.81%, respectively, and their litter size per parity was 1.77 and 2.40, respectively. In the breeding population, the frequencies of B ＋ and BB genotypes were 51.79% and 40.18%, respectively, and their litter size per parity was 2.54 and 3.02, respectively. [ Conclusion] The ovine BMPR-IB gene can be used as a molecular Qenetic marker for fecundity traits to establish high-fecundity Small Tail Han sheep.
