调查四川省一些实验动物养殖场的实验动物中金黄色葡萄球菌(Staphylococcus aureus,SA)携带情况,找出传播途径和进化规律,分析其污染原因,为保障实验动物质量提供技术支撑。按照GB/T 14926.14-2001《实验动物金黄色葡萄球菌检测方法》对...调查四川省一些实验动物养殖场的实验动物中金黄色葡萄球菌(Staphylococcus aureus,SA)携带情况,找出传播途径和进化规律,分析其污染原因,为保障实验动物质量提供技术支撑。按照GB/T 14926.14-2001《实验动物金黄色葡萄球菌检测方法》对2011年-2017年四川省部分实验动物中SA进行检测,对分离的SA菌株进行PCR耐药基因mecA检测,并应用金黄色葡萄球菌A蛋白(Staphylococcus protein A,SPA)和脉冲场电泳(pulsed field gel electrophoresis,PFGE)进行分子分型。结果显示,2011年-2017年共从7家实验动物养殖场采集并完成370只实验动物中SA的检测,共检出SA 31株,总分离率为12.16%,且均从SPF大鼠中分离所得。31株SA的mecA基因均为阴性。SPA可分成5个型别,其中T2360为优势型别,PFGE型别有10个带型。四川省养殖实验动物中仅SPF大鼠携带SA,不同年份检出SA基因型别基本不同,不同养殖场同一年检出的SA具有相同基因型,说明不同养殖场中的SA可能来自同一污染源,应加强SPF大鼠监控,以确保实验动物的质量。展开更多
为精细化把握城市建设项目在微观空间尺度下的停车需求规律,从空间视角探究停车需求与建成环境之间的关系。通过高峰小时建筑物单位面积的停车生成数表征停车需求,以土地利用混合度、路网密度、公交服务水平等9个因子描述建成环境,分别...为精细化把握城市建设项目在微观空间尺度下的停车需求规律,从空间视角探究停车需求与建成环境之间的关系。通过高峰小时建筑物单位面积的停车生成数表征停车需求,以土地利用混合度、路网密度、公交服务水平等9个因子描述建成环境,分别构建建成环境对停车需求影响的普通最小二乘(Ordinary Least Squares,OLS)模型与梯度提升迭代决策树(Gradient Boosting Decision Tree,GBDT)模型。以保定市主城区停车调查数据中的商业类配建停车场为对象,基于停车调查数据、兴趣点数据(Point of Interst,POI)、道路网络数据等多源异构数据进行模型实证分析。结果表明,考虑非线性效应的GBDT模型比OLS模型具有更好的拟合度。从影响贡献度来看,配建指标(18.92%)与区位(15.23%)是影响停车需求的最重要建成环境因素,交叉口密度(5.19%)贡献度最小;在非线性关系方面,建成环境因子与停车需求均具有非线性关系与阈值效应,除交叉口密度及人口密度与停车需求呈现U型关系,其余因素与停车需求的关系整体上保持正相关或负相关。展开更多
Parallel DNA helices with reverse WatsonCrick pairing have been described in several papers.It has been pointed out,however,that the parallel structures may also be in equilibrium with antiparallel WatsonCrick helices...Parallel DNA helices with reverse WatsonCrick pairing have been described in several papers.It has been pointed out,however,that the parallel structures may also be in equilibrium with antiparallel WatsonCrick helices due to their closely similar properties.To avoid the problem we have prepared and characterized DNA helices which are constrained to possess parallel polarity by two dC residues,or CC+clamps,at both the ends of AT oligomer chains.The residues are hemiprotonated below neutral pH,forming two stable CC+base pairs with three hydrogen bonds at each end of the helix.The physical properties of these constructs can then be used to calibrate those of duplexes having the same sequences but lacking the terminal C residues.Though recent studies indicate that dC acid selfstructures are fourstranded with intercalated bases,gel electrophoresis indicates that these structures are not present in the molecules described here.展开更多
文摘调查四川省一些实验动物养殖场的实验动物中金黄色葡萄球菌(Staphylococcus aureus,SA)携带情况,找出传播途径和进化规律,分析其污染原因,为保障实验动物质量提供技术支撑。按照GB/T 14926.14-2001《实验动物金黄色葡萄球菌检测方法》对2011年-2017年四川省部分实验动物中SA进行检测,对分离的SA菌株进行PCR耐药基因mecA检测,并应用金黄色葡萄球菌A蛋白(Staphylococcus protein A,SPA)和脉冲场电泳(pulsed field gel electrophoresis,PFGE)进行分子分型。结果显示,2011年-2017年共从7家实验动物养殖场采集并完成370只实验动物中SA的检测,共检出SA 31株,总分离率为12.16%,且均从SPF大鼠中分离所得。31株SA的mecA基因均为阴性。SPA可分成5个型别,其中T2360为优势型别,PFGE型别有10个带型。四川省养殖实验动物中仅SPF大鼠携带SA,不同年份检出SA基因型别基本不同,不同养殖场同一年检出的SA具有相同基因型,说明不同养殖场中的SA可能来自同一污染源,应加强SPF大鼠监控,以确保实验动物的质量。
文摘为精细化把握城市建设项目在微观空间尺度下的停车需求规律,从空间视角探究停车需求与建成环境之间的关系。通过高峰小时建筑物单位面积的停车生成数表征停车需求,以土地利用混合度、路网密度、公交服务水平等9个因子描述建成环境,分别构建建成环境对停车需求影响的普通最小二乘(Ordinary Least Squares,OLS)模型与梯度提升迭代决策树(Gradient Boosting Decision Tree,GBDT)模型。以保定市主城区停车调查数据中的商业类配建停车场为对象,基于停车调查数据、兴趣点数据(Point of Interst,POI)、道路网络数据等多源异构数据进行模型实证分析。结果表明,考虑非线性效应的GBDT模型比OLS模型具有更好的拟合度。从影响贡献度来看,配建指标(18.92%)与区位(15.23%)是影响停车需求的最重要建成环境因素,交叉口密度(5.19%)贡献度最小;在非线性关系方面,建成环境因子与停车需求均具有非线性关系与阈值效应,除交叉口密度及人口密度与停车需求呈现U型关系,其余因素与停车需求的关系整体上保持正相关或负相关。
文摘Parallel DNA helices with reverse WatsonCrick pairing have been described in several papers.It has been pointed out,however,that the parallel structures may also be in equilibrium with antiparallel WatsonCrick helices due to their closely similar properties.To avoid the problem we have prepared and characterized DNA helices which are constrained to possess parallel polarity by two dC residues,or CC+clamps,at both the ends of AT oligomer chains.The residues are hemiprotonated below neutral pH,forming two stable CC+base pairs with three hydrogen bonds at each end of the helix.The physical properties of these constructs can then be used to calibrate those of duplexes having the same sequences but lacking the terminal C residues.Though recent studies indicate that dC acid selfstructures are fourstranded with intercalated bases,gel electrophoresis indicates that these structures are not present in the molecules described here.
