A stain-based screening method was developed to screen different catalyst coatings for their germicidal activity. A Baclight dead/live bacteria viability kit （invitrogen, molecular probes） was used for staining the ...A stain-based screening method was developed to screen different catalyst coatings for their germicidal activity. A Baclight dead/live bacteria viability kit （invitrogen, molecular probes） was used for staining the cell. The screening was carried out following a standard procedure. This included loading cell suspension to solid surface and maintaining contact for 30 min, then staining with a mixture containing dyes. The stained cells were observed using an epifluorescent microscope and photographed with a CCD camera under UV. Metal-doped TiO2 coatings on AI plates were prepared and tested for non-UV germicidal activity without using UV. It was tested using model microorganisms such as Bakers Yeast （Saccharomyces cerevisiae）, Bacillus subtilis, Pseudomonas putida, and Escherichia coli. On the basis of the germicidal activity of catalyst and the degree of damage caused to the cells, the stained cells may appear green （viable）, green with red or yellow nuclei and yellow （compromised） or red （nonviable）. According to their stained color, cells were counted to calculate the percentage of dead, live, and compromised cells. Compromised cells are cells that grow very slowly after reculturing indicating a degree of reversible cell damage. Screening the germicidal activity using this staining method is accurate and efficient, and requires less time than the culture-based method. A modification to the procedure for measuring germicidal activity of rough surfaces or fibrous coatings was developed. Both TiO2 and metal-doped TiO2 （Ag, Pt, Au, Cu） possess non-UV based germicidal activity. The germicidal activity of TiO2 was found to be related with its wetting property and can be improved by UV irradiation before testing. It is not greatly affected by contact time, indicating a fast acting germicidal activity.展开更多
目的分析胰岛素联合化痰解瘀汤治疗血糖控制不佳的2型糖尿病的临床效果。方法80例血糖控制不佳的2型糖尿病患者,按照随机数字表法分为对照组和观察组,每组40例。对照组给予胰岛素治疗,观察组在对照组基础上联合化痰解瘀汤治疗。比较两...目的分析胰岛素联合化痰解瘀汤治疗血糖控制不佳的2型糖尿病的临床效果。方法80例血糖控制不佳的2型糖尿病患者,按照随机数字表法分为对照组和观察组,每组40例。对照组给予胰岛素治疗,观察组在对照组基础上联合化痰解瘀汤治疗。比较两组患者血糖指标、不良反应发生情况、血脂指标及代谢指标。结果治疗后,观察组患者空腹血糖水平(6.7±1.2)mmol/L及餐后2 h血糖水平(8.2±2.2)mmol/L均低于对照组的(7.6±1.1)、(10.6±3.2)mmol/L,差异具有统计学意义(P<0.05)。观察组患者不良反应发生率与对照组比较,差异无统计学意义(P>0.05)。观察组患者低密度脂蛋白胆固醇、总胆固醇、甘油三酯水平均低于对照组,高密度脂蛋白胆固醇水平高于对照组,差异具有统计学意义(P<0.05)。观察组患者空腹C肽、餐后2 h C肽、糖化血红蛋白水平均低于对照组,差异有统计学意义(P<0.05)。结论给予血糖控制不佳的2型糖尿病患者胰岛素联合化痰解瘀汤治疗,其血糖指标改善明显,对患者的代谢功能具有调节作用,而且治疗安全性较理想,具有推广价值。展开更多
基金Project supported by the HK Innovation and Technology Fund.
文摘A stain-based screening method was developed to screen different catalyst coatings for their germicidal activity. A Baclight dead/live bacteria viability kit （invitrogen, molecular probes） was used for staining the cell. The screening was carried out following a standard procedure. This included loading cell suspension to solid surface and maintaining contact for 30 min, then staining with a mixture containing dyes. The stained cells were observed using an epifluorescent microscope and photographed with a CCD camera under UV. Metal-doped TiO2 coatings on AI plates were prepared and tested for non-UV germicidal activity without using UV. It was tested using model microorganisms such as Bakers Yeast （Saccharomyces cerevisiae）, Bacillus subtilis, Pseudomonas putida, and Escherichia coli. On the basis of the germicidal activity of catalyst and the degree of damage caused to the cells, the stained cells may appear green （viable）, green with red or yellow nuclei and yellow （compromised） or red （nonviable）. According to their stained color, cells were counted to calculate the percentage of dead, live, and compromised cells. Compromised cells are cells that grow very slowly after reculturing indicating a degree of reversible cell damage. Screening the germicidal activity using this staining method is accurate and efficient, and requires less time than the culture-based method. A modification to the procedure for measuring germicidal activity of rough surfaces or fibrous coatings was developed. Both TiO2 and metal-doped TiO2 （Ag, Pt, Au, Cu） possess non-UV based germicidal activity. The germicidal activity of TiO2 was found to be related with its wetting property and can be improved by UV irradiation before testing. It is not greatly affected by contact time, indicating a fast acting germicidal activity.
文摘目的分析胰岛素联合化痰解瘀汤治疗血糖控制不佳的2型糖尿病的临床效果。方法80例血糖控制不佳的2型糖尿病患者,按照随机数字表法分为对照组和观察组,每组40例。对照组给予胰岛素治疗,观察组在对照组基础上联合化痰解瘀汤治疗。比较两组患者血糖指标、不良反应发生情况、血脂指标及代谢指标。结果治疗后,观察组患者空腹血糖水平(6.7±1.2)mmol/L及餐后2 h血糖水平(8.2±2.2)mmol/L均低于对照组的(7.6±1.1)、(10.6±3.2)mmol/L,差异具有统计学意义(P<0.05)。观察组患者不良反应发生率与对照组比较,差异无统计学意义(P>0.05)。观察组患者低密度脂蛋白胆固醇、总胆固醇、甘油三酯水平均低于对照组,高密度脂蛋白胆固醇水平高于对照组,差异具有统计学意义(P<0.05)。观察组患者空腹C肽、餐后2 h C肽、糖化血红蛋白水平均低于对照组,差异有统计学意义(P<0.05)。结论给予血糖控制不佳的2型糖尿病患者胰岛素联合化痰解瘀汤治疗,其血糖指标改善明显,对患者的代谢功能具有调节作用,而且治疗安全性较理想,具有推广价值。
