This study investigated the effects of ammonia-N(control and 1 and 5 mg L^(-1)) on the changes of metabolic enzymes activities and nitrogenous compounds levels in the gills, hepatopancreas, muscles, and hemolymph of t...This study investigated the effects of ammonia-N(control and 1 and 5 mg L^(-1)) on the changes of metabolic enzymes activities and nitrogenous compounds levels in the gills, hepatopancreas, muscles, and hemolymph of the swimming crab Portunus trituberculatus. Results showed that the levels of hemolymph ammonia increased significantly at 6 h and then decreased to control level at 12 h. The activities of key enzymes involved in biosynthesis pathways of glutamine(Gln) and urea, such as glutamate dehydrogenase(GDH), glutamine synthetase(GS), and arginase(ARG), increased remarkably in different tissues of P. trituberculatus. Gln level was the highest in muscles, while urea content was the largest in hepatopancreas. The m RNA expression level of xanthine dehydrogenase(XDH) was downregulated sharply in gills but upregulated in the hepatopancreas and muscles. A small amount of uric acid was detected in the hemolymph. This result suggests that Gln in muscles may be used as an intermediary nitrogen repository, while urea may be primarily synthesized in the hepatopancreas and then transferred to other tissues via the hemolymph. Uric acid could be synthesized by the anabolism of purine nucleotides, in additon to the inhibitory activities of gills. In addition, the levels of Gln, urea and uric acid exhibited dose-and time-dependent effects with ammonia content. Therefore, P. trituberculatus could possibly convert ammonia into Gln, urea, or uric acid to detoxify ammonia during high ambient ammonia-N stress.展开更多
Wavelength selective switch(WSS)is the crucial component in the reconfigurable optical add/drop multiplexer(ROADM),which plays a pivotal role in the next-generation all-optical networks.We present a compact architectu...Wavelength selective switch(WSS)is the crucial component in the reconfigurable optical add/drop multiplexer(ROADM),which plays a pivotal role in the next-generation all-optical networks.We present a compact architecture of twin 1×40 liquid crystal on silicon(LCoS)-based WSS,which can be regarded as a 4f system in the wavelength direction and a 2f system in the switching direction.It is designed with theoretical analysis and simulation investigation.Polarization multiplexing is employed for two sources of twin WSS by polarization con-version before the common optical path.The WSS system attains a coupling efficacy exceeding 96%for 90%of the ports through simulation optimization.The 3 dB bandwidth can be achieved by more than 44 GHz at a 50 GHz grid for all 120 channels at all deflection ports.This work establishes a solid foundation for developing high-performance WSS with larger port counts.展开更多
基金supported by the Natural Science Foundation of Shandong Province, China (No. ZR2016 CM21)
文摘This study investigated the effects of ammonia-N(control and 1 and 5 mg L^(-1)) on the changes of metabolic enzymes activities and nitrogenous compounds levels in the gills, hepatopancreas, muscles, and hemolymph of the swimming crab Portunus trituberculatus. Results showed that the levels of hemolymph ammonia increased significantly at 6 h and then decreased to control level at 12 h. The activities of key enzymes involved in biosynthesis pathways of glutamine(Gln) and urea, such as glutamate dehydrogenase(GDH), glutamine synthetase(GS), and arginase(ARG), increased remarkably in different tissues of P. trituberculatus. Gln level was the highest in muscles, while urea content was the largest in hepatopancreas. The m RNA expression level of xanthine dehydrogenase(XDH) was downregulated sharply in gills but upregulated in the hepatopancreas and muscles. A small amount of uric acid was detected in the hemolymph. This result suggests that Gln in muscles may be used as an intermediary nitrogen repository, while urea may be primarily synthesized in the hepatopancreas and then transferred to other tissues via the hemolymph. Uric acid could be synthesized by the anabolism of purine nucleotides, in additon to the inhibitory activities of gills. In addition, the levels of Gln, urea and uric acid exhibited dose-and time-dependent effects with ammonia content. Therefore, P. trituberculatus could possibly convert ammonia into Gln, urea, or uric acid to detoxify ammonia during high ambient ammonia-N stress.
基金This work was supported by ZTE Industry⁃University⁃Institute Coopera⁃tion Funds under Grant No.IA20230614004.
文摘Wavelength selective switch(WSS)is the crucial component in the reconfigurable optical add/drop multiplexer(ROADM),which plays a pivotal role in the next-generation all-optical networks.We present a compact architecture of twin 1×40 liquid crystal on silicon(LCoS)-based WSS,which can be regarded as a 4f system in the wavelength direction and a 2f system in the switching direction.It is designed with theoretical analysis and simulation investigation.Polarization multiplexing is employed for two sources of twin WSS by polarization con-version before the common optical path.The WSS system attains a coupling efficacy exceeding 96%for 90%of the ports through simulation optimization.The 3 dB bandwidth can be achieved by more than 44 GHz at a 50 GHz grid for all 120 channels at all deflection ports.This work establishes a solid foundation for developing high-performance WSS with larger port counts.
