目的通过多肽组学分析,从子宫内膜癌组织中筛选获得具有潜在生物学活性的内源性多肽。方法收集3例子宫内膜样腺癌组织标本(以3例年龄匹配的健康女性子宫内膜样本为对照),通过液相色谱-串联质谱法(liquid chromatography-tandem mass spe...目的通过多肽组学分析,从子宫内膜癌组织中筛选获得具有潜在生物学活性的内源性多肽。方法收集3例子宫内膜样腺癌组织标本(以3例年龄匹配的健康女性子宫内膜样本为对照),通过液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)获得子宫内膜癌特异性内源性多肽,借助生物信息学方法筛选具有潜在活性的功能多肽,使用CCK-8实验、Transwell及划痕实验验证多肽对子宫内膜癌细胞株HEC-1-A增殖、侵袭、迁移的影响,并以PCR、Western Blot方法选择性观察多肽对Wnt/β-catenin/EMT通路相关分子表达的影响。结果鉴定获得序列为TAGNVLMH的多肽(PDAEP-1)对应前体为SLC38A10,其表达水平与子宫内膜癌患者5年生存率相关;该多肽对子宫内膜癌细胞的增殖、侵袭、迁移有明显的抑制作用,且作用后Vimentin、β-catenin两者的mRNA及蛋白水平均显著降低,而E-cadherin则上调。结论通过调变Wnt/β-catenin/EMT通路相关分子,内源性多肽PDAEP-1在体外有抑制子宫内膜癌细胞增殖、侵袭和迁移的抗肿瘤活性,具有潜在的诊疗应用价值。展开更多
This paper addresses the emergence of water security problems in North China with the aim of highlighting key waterresources management and water security issues for the long-term development of North China. Three key...This paper addresses the emergence of water security problems in North China with the aim of highlighting key waterresources management and water security issues for the long-term development of North China. Three key problemsrelated to water resources and security issues in North China in the 21st century are addressed, namely 1) the watercycle under environmental change, 2) agricultural water saving, and 3) water security. Development of internationalresearch related to these issues is also reviewed. The research plan developed recently by the Chinese Academy of Sciences(CAS) is discussed and suggestions on research and development of water resources science in North China are presented.Thanks to focus on experimental catchments and dedicated research stations, a detailed knowledge of the water cycle onNorth China farmland has been compiled. A range of techniques that include isotope tracers has been used to acquirehydrologic data. Much research has been devoted to developing distributed hydrological models at different scales. In thewell irrigation district, five different water saving irrigation regimes have been investigated, and these regimes have hadwidespread application, and reduced water use 60-150 mm while they increased water use efficiency (WUE) by 20%-30%.Furthermore, preventing water pollution is the most essential step to ensure North China’s water security.展开更多
文摘目的通过多肽组学分析,从子宫内膜癌组织中筛选获得具有潜在生物学活性的内源性多肽。方法收集3例子宫内膜样腺癌组织标本(以3例年龄匹配的健康女性子宫内膜样本为对照),通过液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)获得子宫内膜癌特异性内源性多肽,借助生物信息学方法筛选具有潜在活性的功能多肽,使用CCK-8实验、Transwell及划痕实验验证多肽对子宫内膜癌细胞株HEC-1-A增殖、侵袭、迁移的影响,并以PCR、Western Blot方法选择性观察多肽对Wnt/β-catenin/EMT通路相关分子表达的影响。结果鉴定获得序列为TAGNVLMH的多肽(PDAEP-1)对应前体为SLC38A10,其表达水平与子宫内膜癌患者5年生存率相关;该多肽对子宫内膜癌细胞的增殖、侵袭、迁移有明显的抑制作用,且作用后Vimentin、β-catenin两者的mRNA及蛋白水平均显著降低,而E-cadherin则上调。结论通过调变Wnt/β-catenin/EMT通路相关分子,内源性多肽PDAEP-1在体外有抑制子宫内膜癌细胞增殖、侵袭和迁移的抗肿瘤活性,具有潜在的诊疗应用价值。
基金the Knowledge Innovation Key Project of the Chinese Academy of Sciences (Nos. KZCX2-SW-317/CX10G-E01-08 and KZCX1-09-02) and the National Natural Science Foundation of China (No. 50279049).
文摘This paper addresses the emergence of water security problems in North China with the aim of highlighting key waterresources management and water security issues for the long-term development of North China. Three key problemsrelated to water resources and security issues in North China in the 21st century are addressed, namely 1) the watercycle under environmental change, 2) agricultural water saving, and 3) water security. Development of internationalresearch related to these issues is also reviewed. The research plan developed recently by the Chinese Academy of Sciences(CAS) is discussed and suggestions on research and development of water resources science in North China are presented.Thanks to focus on experimental catchments and dedicated research stations, a detailed knowledge of the water cycle onNorth China farmland has been compiled. A range of techniques that include isotope tracers has been used to acquirehydrologic data. Much research has been devoted to developing distributed hydrological models at different scales. In thewell irrigation district, five different water saving irrigation regimes have been investigated, and these regimes have hadwidespread application, and reduced water use 60-150 mm while they increased water use efficiency (WUE) by 20%-30%.Furthermore, preventing water pollution is the most essential step to ensure North China’s water security.
