标本来自外科手术取出的新鲜乳腺涂片,经病理学确诊后获得32例。应用显微分光光度计技术,对各种乳腺疾患的单个细胞的间期核进行DNA含量测定。以2例正常人耳血和4例乳腺疾患的淋巴细胞,作为二倍体细胞的DNA含量的对照参考值。测得正常...标本来自外科手术取出的新鲜乳腺涂片,经病理学确诊后获得32例。应用显微分光光度计技术,对各种乳腺疾患的单个细胞的间期核进行DNA含量测定。以2例正常人耳血和4例乳腺疾患的淋巴细胞,作为二倍体细胞的DNA含量的对照参考值。测得正常淋巴细胞DNA平均值为11.22±0.2 A.U和10.98±1.3 A U。乳腺病灶旁组织10例,其上皮核DNA平均值为12.5±1.3 A U;乳腺增生5例,为21.36±1.6 A U;乳腺纤维瘤9例,为22.61±1.0 A.U;乳腺癌8例,为31.9±2.3 A.U。本文认为,乳腺癌细胞的DNA值表现不正常,多倍体细胞增多,组方图右移,带双峰或多峰的非整倍体分布图形。良性乳腺病的DNA含量,比癌细胞低得多,但比正常细胞高,它们的DNA峰值位于二倍体~四倍体之间。我们认为,应用DNA原位定量技术,对癌变细胞的早期和预后诊断,以及抗癌药物疗效的判断,有重要的参考价值。展开更多
背景:对于3-磷酸肌醇依赖性蛋白激酶1(3-phosphoinositide-dependent protein kinase 1,PDK1)的学术研究,国内外主要集中在内分泌和肿瘤学等学科领域,而在骨科学中关于调控破骨细胞对骨质疏松的改善尚未有系统研究与报道。目的:探索PDK...背景:对于3-磷酸肌醇依赖性蛋白激酶1(3-phosphoinositide-dependent protein kinase 1,PDK1)的学术研究,国内外主要集中在内分泌和肿瘤学等学科领域,而在骨科学中关于调控破骨细胞对骨质疏松的改善尚未有系统研究与报道。目的:探索PDK1调控破骨细胞对骨质疏松症发病的影响及分子机制,为临床防治骨质疏症提供新的药物靶点。方法:提取PDK1基因条件性敲除型小鼠(PDK1-cKO)及野生型C57小鼠全骨髓细胞并诱导分化为破骨细胞,将诱导的破骨细胞进行抗酒石酸酸性磷酸酶染色,观察两组破骨细胞的数量及形态变化,蛋白免疫印迹法检测PDK1基因敲除后调控破骨细胞分化相关蛋白的表达。构建两组小鼠卵巢切除模型,采用Micro-CT扫描、抗酒石酸酸性磷酸酶染色来观察PDK1基因敲除对骨质疏松症的影响。结果与结论:①抗酒石酸酸性磷酸酶染色结果:相比较于野生型组,PDK1-cKO组破骨细胞数量在第4,6天显著减少(P<0.05);②蛋白免疫印迹结果显示:PDK1-cKO组调控破骨细胞分化的关键蛋白AKT磷酸化水平下降(P<0.05);③Micro-CT扫描结果:PDK1-cKO组比野生型组骨质疏松情况明显减轻(P<0.05);④抗酒石酸酸性磷酸酶染色结果:PDK1-cKO组胫骨远端破骨细胞较野生型组明显减少(P<0.05);⑤结果显示:PDK1基因可通过调控破骨细胞分化、优化骨代谢平衡来改善骨质疏松症状,有作为治疗骨质疏松疾病药物靶点的可能。展开更多
OBJECTIVE:To systematically evaluate the efficacy and safety of acupuncture(AM)in the treatment of pregnancyrelated symptoms such as acute vomiting during pregnancy.METHODS:We comprehensively searched the available li...OBJECTIVE:To systematically evaluate the efficacy and safety of acupuncture(AM)in the treatment of pregnancyrelated symptoms such as acute vomiting during pregnancy.METHODS:We comprehensively searched the available literature up to November 2021,including Pub Med,Embase,Cochrane Library,Chinese Biomedical Literature Database,and China National Knowledge Infrastructure Database,for randomized controlled trials(RCTs)on AM for the treatment of severe vomiting,insomnia,pharyngeal and pelvic pain,mood abnormalities,and dyspepsia during pregnancy.RESULTS:Sixteen RCTs with a cumulative sample size of 1178 cases were included.Of these,964 patients were included in the Meta-analysis.The Meta-analysis results showed that AM was more efficient than Western medicine in treating discomfort during pregnancy[odds ratio(OR)=1.19,95%confidence interval(CI)(1.11,1.28),P<0.01].AM was better than the control group in improving the visual analog scale scores[standard mean difference(SMD)=0.62,95%CI(0.53,0.71),P<0.01].AM was superior to the control group in improving Numerical Rating Scale(NRS)symptom scores[OR=7.31,95%CI(3.36,15.94),P<0.01].There was no significant difference in adverse effects between the AM and sham-AM groups and the analgesic drug group[OR=0.70,95%CI(0.39,1.28),P=0.25],but the treatment and control groups had mild adverse effects with a low incidence.CONCLUSIONS:AM is more effective than other treatments or pharmacotherapy alone in the treatment of pregnancy-related symptoms,and is relatively safe.However,the quality of the included trials was rather poor,and high-quality studies are required to confirm our findings.展开更多
文摘标本来自外科手术取出的新鲜乳腺涂片,经病理学确诊后获得32例。应用显微分光光度计技术,对各种乳腺疾患的单个细胞的间期核进行DNA含量测定。以2例正常人耳血和4例乳腺疾患的淋巴细胞,作为二倍体细胞的DNA含量的对照参考值。测得正常淋巴细胞DNA平均值为11.22±0.2 A.U和10.98±1.3 A U。乳腺病灶旁组织10例,其上皮核DNA平均值为12.5±1.3 A U;乳腺增生5例,为21.36±1.6 A U;乳腺纤维瘤9例,为22.61±1.0 A.U;乳腺癌8例,为31.9±2.3 A.U。本文认为,乳腺癌细胞的DNA值表现不正常,多倍体细胞增多,组方图右移,带双峰或多峰的非整倍体分布图形。良性乳腺病的DNA含量,比癌细胞低得多,但比正常细胞高,它们的DNA峰值位于二倍体~四倍体之间。我们认为,应用DNA原位定量技术,对癌变细胞的早期和预后诊断,以及抗癌药物疗效的判断,有重要的参考价值。
文摘背景:对于3-磷酸肌醇依赖性蛋白激酶1(3-phosphoinositide-dependent protein kinase 1,PDK1)的学术研究,国内外主要集中在内分泌和肿瘤学等学科领域,而在骨科学中关于调控破骨细胞对骨质疏松的改善尚未有系统研究与报道。目的:探索PDK1调控破骨细胞对骨质疏松症发病的影响及分子机制,为临床防治骨质疏症提供新的药物靶点。方法:提取PDK1基因条件性敲除型小鼠(PDK1-cKO)及野生型C57小鼠全骨髓细胞并诱导分化为破骨细胞,将诱导的破骨细胞进行抗酒石酸酸性磷酸酶染色,观察两组破骨细胞的数量及形态变化,蛋白免疫印迹法检测PDK1基因敲除后调控破骨细胞分化相关蛋白的表达。构建两组小鼠卵巢切除模型,采用Micro-CT扫描、抗酒石酸酸性磷酸酶染色来观察PDK1基因敲除对骨质疏松症的影响。结果与结论:①抗酒石酸酸性磷酸酶染色结果:相比较于野生型组,PDK1-cKO组破骨细胞数量在第4,6天显著减少(P<0.05);②蛋白免疫印迹结果显示:PDK1-cKO组调控破骨细胞分化的关键蛋白AKT磷酸化水平下降(P<0.05);③Micro-CT扫描结果:PDK1-cKO组比野生型组骨质疏松情况明显减轻(P<0.05);④抗酒石酸酸性磷酸酶染色结果:PDK1-cKO组胫骨远端破骨细胞较野生型组明显减少(P<0.05);⑤结果显示:PDK1基因可通过调控破骨细胞分化、优化骨代谢平衡来改善骨质疏松症状,有作为治疗骨质疏松疾病药物靶点的可能。
基金Project of Science and Technology Department of Hubei Province in 2023:to Investigate the Therapeutic Mechanism of Qingre Lishi Huayu Herbs on Pelvic Inflammatory Disease Based on NOTCH Signaling Pathway(No.2023AFD167)。
文摘OBJECTIVE:To systematically evaluate the efficacy and safety of acupuncture(AM)in the treatment of pregnancyrelated symptoms such as acute vomiting during pregnancy.METHODS:We comprehensively searched the available literature up to November 2021,including Pub Med,Embase,Cochrane Library,Chinese Biomedical Literature Database,and China National Knowledge Infrastructure Database,for randomized controlled trials(RCTs)on AM for the treatment of severe vomiting,insomnia,pharyngeal and pelvic pain,mood abnormalities,and dyspepsia during pregnancy.RESULTS:Sixteen RCTs with a cumulative sample size of 1178 cases were included.Of these,964 patients were included in the Meta-analysis.The Meta-analysis results showed that AM was more efficient than Western medicine in treating discomfort during pregnancy[odds ratio(OR)=1.19,95%confidence interval(CI)(1.11,1.28),P<0.01].AM was better than the control group in improving the visual analog scale scores[standard mean difference(SMD)=0.62,95%CI(0.53,0.71),P<0.01].AM was superior to the control group in improving Numerical Rating Scale(NRS)symptom scores[OR=7.31,95%CI(3.36,15.94),P<0.01].There was no significant difference in adverse effects between the AM and sham-AM groups and the analgesic drug group[OR=0.70,95%CI(0.39,1.28),P=0.25],but the treatment and control groups had mild adverse effects with a low incidence.CONCLUSIONS:AM is more effective than other treatments or pharmacotherapy alone in the treatment of pregnancy-related symptoms,and is relatively safe.However,the quality of the included trials was rather poor,and high-quality studies are required to confirm our findings.
