Background Our previous in vivo study in the rat demonstrates that Shenfu injection, a clinically used extract preparation from Chinese herbs, attenuates neural and cardiac toxicity induced by intravenous infusion of ...Background Our previous in vivo study in the rat demonstrates that Shenfu injection, a clinically used extract preparation from Chinese herbs, attenuates neural and cardiac toxicity induced by intravenous infusion of bupivacaine, a local anesthetic. This study was designed to investigate whether bupivacaine could induce a toxic effect in pnmary cultured mouse spinal cord neuron and if so, whether the Shenfu injection had a similar neuroprotective effect in the cell model. Methods The spinal cords from 11 - to 14-day-old fetal mice were minced and incubated. Cytarabine was added into the medium to inhibit the proliferation of non-neuronal cells. The immunocytochemical staining of β-tubulin was used to determine the identity of cultured cells. The cultured neurons were randomly assigned into three sets treated with various doses of bupivacaine, Shenfu and bupivacaine＋Shenfu, for 48 hours respectively. Cell viability in each group was analyzed by methyl thiazoleterazolium （MTT） assay. Results The viability of the cultured neurons treated with bupivacaine at concentrations of 0.01%, 0.02%, 0.04% and 0.08% was decreased in a dose-dependent manner. Although the Shenfu injection at concentrations ranging from 1/50 to 1/12.5 （VN） had no significant influence on the viability of cultured neurons （P〈0.05 vs control）, the injection significantly increased the cellular viability of cultured neurons pretreated with 0.03% bupivacaine （P〈0.05）. Conclusion Although Shenfu injection itself has no effect on spinal neurons, it was able to reduce the bupivacaineinduced neurotoxicity in vitro.展开更多
基金This work was supported by a grant from the Ministry of Health of China(No.200312)
文摘Background Our previous in vivo study in the rat demonstrates that Shenfu injection, a clinically used extract preparation from Chinese herbs, attenuates neural and cardiac toxicity induced by intravenous infusion of bupivacaine, a local anesthetic. This study was designed to investigate whether bupivacaine could induce a toxic effect in pnmary cultured mouse spinal cord neuron and if so, whether the Shenfu injection had a similar neuroprotective effect in the cell model. Methods The spinal cords from 11 - to 14-day-old fetal mice were minced and incubated. Cytarabine was added into the medium to inhibit the proliferation of non-neuronal cells. The immunocytochemical staining of β-tubulin was used to determine the identity of cultured cells. The cultured neurons were randomly assigned into three sets treated with various doses of bupivacaine, Shenfu and bupivacaine＋Shenfu, for 48 hours respectively. Cell viability in each group was analyzed by methyl thiazoleterazolium （MTT） assay. Results The viability of the cultured neurons treated with bupivacaine at concentrations of 0.01%, 0.02%, 0.04% and 0.08% was decreased in a dose-dependent manner. Although the Shenfu injection at concentrations ranging from 1/50 to 1/12.5 （VN） had no significant influence on the viability of cultured neurons （P〈0.05 vs control）, the injection significantly increased the cellular viability of cultured neurons pretreated with 0.03% bupivacaine （P〈0.05）. Conclusion Although Shenfu injection itself has no effect on spinal neurons, it was able to reduce the bupivacaineinduced neurotoxicity in vitro.
