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Qualitative and Quantitative Analysis of Alkaloids in Cortex Phellodendri by HPLC-ESI-MS/MS and HPLC-DAD 被引量:19
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作者 ZHU Shuang-lai DOU Sheng-shan +6 位作者 liu Xin-ru liu run-hui ZHANG Wei-dong HUANG Hong-lin ZHANG Yi HU Yao-hua WANG Shu-ping 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2011年第1期38-44,共7页
A combined method of high performance liquid chromatograph-elecrtrospray-ionization mass spectrometer(HPLC-ESI-MS/MS) coupled with a photodiode array detector(HPLC-DAD) and principal component analysis(PCA) was ... A combined method of high performance liquid chromatograph-elecrtrospray-ionization mass spectrometer(HPLC-ESI-MS/MS) coupled with a photodiode array detector(HPLC-DAD) and principal component analysis(PCA) was applied to the qualitative and quantitative analyses of alkaloids in Cortex Phellodendri(CP) samples, and to the differentiation of two species of CP, Cortex Phellodendri Chinensis(CPC) and Cortex Phellodendri Amurensis(CPA). Twenty-two peaks appeared in the HPLC-MS base peak chromatogram of CP detected by the HPLC-ESI-MS/MS analysis, and the alkaloids were identified according to the MSn data, the known MS fragmentation rules and the literature data. Five alkaloids including berberine, palmatine, jatrorrhizine, phellodendrine and magnoflorine were simultaneously determinated by the HPLC-DAD. Berberine was the primary component in all CP samples, and the contents of berberine and palmatine were exploited to be two critical parameters for effective discrimination between the two species of CP. The average content of berberine in CPC(58.75 mg/g) was higher than that in CPA(9.16 mg/g), while the content of palmatine was less, only 0.25 mg/g in CPC and 4.19 mg/g in CPA. With the use of PCA, samples datasets were separated successfully into two different clusters corresponding to the two species, and berberine, pahnatine, phellodendrine and magnoflorine contribute most to the above mentioned calssifying . The proposed method oroved to be a useful tool in the aualitv control of Chinese herbal medicines. 展开更多
关键词 Cortex Phellodendri ALKALOID HPLC-ESI-MS/MS Quantitative analysis Qualitative analysis Principal component analysis
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中药化学课程思政的教学探索与教学效果实践 被引量:8
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作者 祖先鹏 柳润辉 +1 位作者 徐希科 沈云亨 《医学教育研究与实践》 2021年第4期581-585,共5页
中药化学是具有传统文化特色的学科,历史悠久,底蕴深厚。但在专业教育上尚不能和现代中医药的发展有机融合。根据德育素质要求,教育部提出全方位、全过程的育人理念。在“课程建设”教学体系的建设中,秉承“以人为本,以德立教”的教学理... 中药化学是具有传统文化特色的学科,历史悠久,底蕴深厚。但在专业教育上尚不能和现代中医药的发展有机融合。根据德育素质要求,教育部提出全方位、全过程的育人理念。在“课程建设”教学体系的建设中,秉承“以人为本,以德立教”的教学理念,以中药化学课程的专业课教育和思想文化教育为核心目标。在专业课程的展开上要立足中药化学优势特色,结合中药学专业特征,多方面挖掘中药化学课程各章节中的思政元素,秉承“以学生为中心”的教学理念,因材施教,将德育工作落到实处,通过教学制度改革,加强教师团队建设等方式,深化学生理想信念,增强学生的中药文化自信。目前中药化学课程思政的教学模式仍处于摸索阶段,培养具有社会责任感和大国工匠精神的高素质专业人才仍需要长期的努力。 展开更多
关键词 中药化学 课程思政 教学探索 教学效果
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Qualitative and Quantitative Analysis for the Quality Control of Rhizoma Coptidis by HPLC-DAD and HPLC-ESI-MS 被引量:1
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作者 DOU Sheng-shan ZHU Shuang-lai +3 位作者 DAI Wei-xing ZHANG Wei-dong ZHANG Yi liu run-hui 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2010年第5期735-741,共7页
For quality control purpose, an approach of fingerprinting and simultaneous quantification of five major bioactive constituents of Rhizoma Coptidis was established via a high-performance liquid chromatograph coupled w... For quality control purpose, an approach of fingerprinting and simultaneous quantification of five major bioactive constituents of Rhizoma Coptidis was established via a high-performance liquid chromatograph coupled with a photodiode array UV detector(HPLC-DAD) and an electrospray ionization mass spectrometer(HPLC-ESI/MS) The compounds were identified on the basis of the comparison of their mass spectra with literature data and those of standard samples and quantified by the HPLC-DAD method. Baseline separation was achieved on an XTerra C18 column(5 μm, 250 mm×4.6 mm i. d.) with linear gradient elution of formate buffer(consisting of 0.5% formic acid, adjusted to pH=4.5 with ammonia) and acetonitrile(consisting of 0.2% formic acid and 0.2% triethylamine). The me- thod was validated for linearity(r^2〉0.9995), repeatability(RSD〈3.1%), intra- and inter-day precision(RSD〈1.8%) with recovery(99.9%-105.1%), limits of detection(0.15-0.35 μg/mL), and limits of quantification(0.53-0.82 μg/mL). The similarities of 32 batches of Rhizoma Coptidis and their classification according to their manufacturers were based on the retention time and peak areas of the characteristic compounds. The five compounds were selected for quality assessment ofRhizoma coptidis via partial least squares analysis(PLS). 展开更多
关键词 Rhizoma Coptidis HPLC-DAD HPLC-MS Partial least squares analysis Quality control
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基于整体观中药质量标志物的发现及研究进展 被引量:29
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作者 叶霁 李睿旻 +2 位作者 曾华武 柳润辉 张卫东 《中草药》 CAS CSCD 北大核心 2019年第19期4529-4537,共9页
中药质量是中医药应用、发展和传承的重要因素,是中药发挥临床疗效的保障。长期以来,具有复杂性和多样性特征的中药,特别是由多种中药组成的复方中药药效物质基础尚不明确,限制了其质量标准的制定,影响了中医药国际化的进程。质量标志... 中药质量是中医药应用、发展和传承的重要因素,是中药发挥临床疗效的保障。长期以来,具有复杂性和多样性特征的中药,特别是由多种中药组成的复方中药药效物质基础尚不明确,限制了其质量标准的制定,影响了中医药国际化的进程。质量标志物主要依据中药属性、制造过程及配伍理论等特点提出,是中药及中药复方质量评价的指标性成分。近些年来关于质量标志物的解析和应用研究得到了迅猛的发展,为质量标准的制定提供更加科学、明确的方向。从中药方剂整体观研究体系出发,围绕中药复方的药理活性以及相关化学物质基础、整合系统生物学和网络药理学的前沿技术,对质量标志物的发现进行综述,介绍相关经验方法和研究进展,以期为发现与临床疗效相关的药效成分即质量标志物提供丰富有益的方法指导。 展开更多
关键词 质量标志物 中药 整体观 药效物质基础 系统生物学 网络药理学
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“Omics” in pharmaceutical research: overview, applications, challenges, and future perspectives 被引量:18
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作者 YAN Shi-Kai liu run-hui +4 位作者 JIN Hui-Zi liu Xin-Ru YE Ji SHAN Lei ZHANG Wei-Dong 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2015年第1期3-21,共19页
In the post-genomic era, biological studies are characterized by the rapid development and wide application of a series of "omics" technologies, including genomics, proteomics, metabolomics, transcriptomics,... In the post-genomic era, biological studies are characterized by the rapid development and wide application of a series of "omics" technologies, including genomics, proteomics, metabolomics, transcriptomics, lipidomics, cytomics, metallomics, ionomics, interactomics, and phenomics. These "omics" are often based on global analyses of biological samples using high through-put analytical approaches and bioinformatics and may provide new insights into biological phenomena. In this paper, the development and advances in these omics made in the past decades are reviewed, especially genomics, transcriptomics, proteomics and metabolomics; the applications of omics technologies in pharmaceutical research are then summarized in the fields of drug target discovery, toxicity evaluation, personalized medicine, and traditional Chinese medicine; and finally, the limitations of omics are discussed, along with the future challenges associated with the multi-omics data processing, dynamics omics analysis, and analytical approaches, as well as amenable solutions and future prospects. 展开更多
关键词 OMICS GENOMICS TRANSCRIPTOMICS PROTEOMICS Metabolomics Target Discovery Toxicity Assessment PersonalizedMedicine Traditional Chinese Medicine
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系统生物学在中药方剂现代研究中的应用 被引量:5
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作者 柳润辉 《中国中西医结合杂志》 CAS CSCD 北大核心 2019年第2期148-150,共3页
中医药学是具有中国特色的生命科学,在我国医药卫生事业中占有十分重要的地位。中药方剂作为中医临床用药的主要形式,是中医药的精华,集中体现了中医学“辨证论治、君臣佐使、扶正祛邪”的理论特色,具有多成分、多途径和多靶点协同作用... 中医药学是具有中国特色的生命科学,在我国医药卫生事业中占有十分重要的地位。中药方剂作为中医临床用药的主要形式,是中医药的精华,集中体现了中医学“辨证论治、君臣佐使、扶正祛邪”的理论特色,具有多成分、多途径和多靶点协同作用的特点,在防治复杂疾病方面具有独特优势。 展开更多
关键词 中药方剂 系统生物学 医药卫生事业 中医临床用药 中医药学 生命科学 中国特色 辨证论治
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