The gene encoding the 18 kDa protein of Taenia solium metacestodes was amplified by RT-PCR and cloned into the pGEM-T vector for sequencing.The recombinant plasmid named pGEX-CE18 was constructed and transformed into ...The gene encoding the 18 kDa protein of Taenia solium metacestodes was amplified by RT-PCR and cloned into the pGEM-T vector for sequencing.The recombinant plasmid named pGEX-CE18 was constructed and transformed into E.coli BL21 for in vitro expression.SDS-PAGE and Western blot were employed for analyzing the recombinant protein,which was then used for development of an indirect ELISA for detection of anti-cysticercosis antibodies.The results showed that the recombinant protein of interest was 35 kDa in size,accounting for 28%of total bacteria proteins,and reacted with positive sera against cysticercosis.Using the newly-constructed indirect ELISA and a commercially available ELISA kit,paired analyses of 178 serum samples indicated that the concordant rate was 98.83%and the ELISA exhibited good specificity and sensitivity,supporting its utility and application for diagnosis of cysticercosis.展开更多
The objective of this study was to investigate the effects of applying different amounts of water and nitrogen on yield, fruit quality, water use efficiency(WUE), irrigation water use efficiency(IWUE) and nitrogen use...The objective of this study was to investigate the effects of applying different amounts of water and nitrogen on yield, fruit quality, water use efficiency(WUE), irrigation water use efficiency(IWUE) and nitrogen use efficiency(NUE) of drip-irrigated greenhouse tomatoes in northwestern China. The plants were irrigated every seven days at various proportions of 20-cm pan evaporation(E_p). The experiment consisted of three irrigation levels(I1, 50% E_p; I2, 75% E_p; and I3, 100% E_p) and three N application levels(N1, 150 kg N ha^(–1); N2, 250 kg N ha^(–1); and N3, 350 kg N ha^(–1)). Tomato yield increased with the amount of applied irrigation water in I2 and then decreased in I3. WUE and IWUE were the highest in I1. WUE was 16.5% lower in I2 than that in I1, but yield was 26.6% higher in I2 than that in I1. Tomato yield, WUE, and IWUE were significantly higher in N2 than that in N1 and N3. NUE decreased with increasing N levels but NUE increased with increase the amount of water applied. Increasing both water and N levels increased the foliar net photosynthetic rate. I1 and I2 treatments significantly increased the contents of total soluble solids(TSS), vitamin C(VC), lycopene, soluble sugars(SS), and organic acids(OA) and the sugar:acid ratio in the fruit and decreased the nitrate content. TSS, VC, lycopene, and SS contents were the highest in N2. The harvest index(HI) was the highest in I2N2. I2N2 provided the optimal combination of tomato yield, fruit quality, and WUE. The irrigation and fertilisation regime of 75% E_p and 250 kg N ha^(–1) was the best strategy of water and N management for the production of drip-irrigated greenhouse tomato.展开更多
基金supported by National High-tech Research and Development Plan(863 Project)(2006AA10A207)
文摘The gene encoding the 18 kDa protein of Taenia solium metacestodes was amplified by RT-PCR and cloned into the pGEM-T vector for sequencing.The recombinant plasmid named pGEX-CE18 was constructed and transformed into E.coli BL21 for in vitro expression.SDS-PAGE and Western blot were employed for analyzing the recombinant protein,which was then used for development of an indirect ELISA for detection of anti-cysticercosis antibodies.The results showed that the recombinant protein of interest was 35 kDa in size,accounting for 28%of total bacteria proteins,and reacted with positive sera against cysticercosis.Using the newly-constructed indirect ELISA and a commercially available ELISA kit,paired analyses of 178 serum samples indicated that the concordant rate was 98.83%and the ELISA exhibited good specificity and sensitivity,supporting its utility and application for diagnosis of cysticercosis.
基金supported by grants from the National High-Tech R&D Program of China(863 Program)(2013AA103004)the Water and Technology Support Plan of Shaanxi Province,China(2014slkj-17)
文摘The objective of this study was to investigate the effects of applying different amounts of water and nitrogen on yield, fruit quality, water use efficiency(WUE), irrigation water use efficiency(IWUE) and nitrogen use efficiency(NUE) of drip-irrigated greenhouse tomatoes in northwestern China. The plants were irrigated every seven days at various proportions of 20-cm pan evaporation(E_p). The experiment consisted of three irrigation levels(I1, 50% E_p; I2, 75% E_p; and I3, 100% E_p) and three N application levels(N1, 150 kg N ha^(–1); N2, 250 kg N ha^(–1); and N3, 350 kg N ha^(–1)). Tomato yield increased with the amount of applied irrigation water in I2 and then decreased in I3. WUE and IWUE were the highest in I1. WUE was 16.5% lower in I2 than that in I1, but yield was 26.6% higher in I2 than that in I1. Tomato yield, WUE, and IWUE were significantly higher in N2 than that in N1 and N3. NUE decreased with increasing N levels but NUE increased with increase the amount of water applied. Increasing both water and N levels increased the foliar net photosynthetic rate. I1 and I2 treatments significantly increased the contents of total soluble solids(TSS), vitamin C(VC), lycopene, soluble sugars(SS), and organic acids(OA) and the sugar:acid ratio in the fruit and decreased the nitrate content. TSS, VC, lycopene, and SS contents were the highest in N2. The harvest index(HI) was the highest in I2N2. I2N2 provided the optimal combination of tomato yield, fruit quality, and WUE. The irrigation and fertilisation regime of 75% E_p and 250 kg N ha^(–1) was the best strategy of water and N management for the production of drip-irrigated greenhouse tomato.
