构建脂肪特异性蛋白27(Fat-specific protein of 27,Fsp27)基因沉默载体,研究沉默Fsp27基因表达对3T3-L1细胞脂解的影响,并对其作用机制进行探究。采用RNAi技术,构建Fsp27基因真核干扰载体,下调Fsp27基因的表达。“鸡尾酒”法诱导3T3-L...构建脂肪特异性蛋白27(Fat-specific protein of 27,Fsp27)基因沉默载体,研究沉默Fsp27基因表达对3T3-L1细胞脂解的影响,并对其作用机制进行探究。采用RNAi技术,构建Fsp27基因真核干扰载体,下调Fsp27基因的表达。“鸡尾酒”法诱导3T3-L1前脂肪细胞分化为成熟脂肪细胞。脂质体转染脂肪细胞,油红O染色脂滴,酶法测定细胞中甘油及甘油三酯的含量。Western blot法检测细胞中Fsp27、HSL、ATGL和PPARγ的蛋白表达。Western blot结果显示:阳性sh-Fsp27干扰载体均能有效下调Fsp27的表达,且伴随细胞内ATGL和PPARγ的表达量升高(P<0.05),其中sh-Fsp27-2的沉默效果最好;酶学方法检测结果显示:阳性sh-Fsp27干扰组细胞中甘油三酯含量下降,甘油含量升高(P<0.05);油红O染色结果发现:空白对照组与阴性对照组均有大脂滴堆积,阳性sh-Fsp27组小脂滴分布广泛,未见明显的大脂滴。sh-Fsp27-2组基因沉默载体的沉默效果最好,Fsp27基因沉默可以加快3T3-L1细胞的脂解速率,其主要是通过抑制脂滴融合和增强ATGL酶的水解来完成对脂解的调控。展开更多
A novel hydrogen-bonded organic framework, HOF-TCPP(HOF = hydrogen bonded organic framework, TCPP = tetrakis(4-carboxyphenyl) porphyrin), has been synthesized via solvothermal reaction in ethylene glycol. Crystal stru...A novel hydrogen-bonded organic framework, HOF-TCPP(HOF = hydrogen bonded organic framework, TCPP = tetrakis(4-carboxyphenyl) porphyrin), has been synthesized via solvothermal reaction in ethylene glycol. Crystal structure was well determined by single-crystal X-ray diffraction and powder X-ray diffraction(PXRD). Topological analysis reveals that HOF-TCPP exhibits sql 2D layer and features 2D → 3D polycatenation. Fluorescence investigation shows that HOF-TCPP displays much higher photoluminescence(PL) intensity than the amorphous ligands TCPP, which can be ascribed to the crystalline structure and hydrogen bonds existing in the structure.展开更多
An interesting metal-organic framework(MOF) PFC-31 was synthesized by a simple solvothermal reaction. X-ray crystallography reveals that PFC-31 crystallizes in monoclinic system, space group P21/c with a = 6.8410(17),...An interesting metal-organic framework(MOF) PFC-31 was synthesized by a simple solvothermal reaction. X-ray crystallography reveals that PFC-31 crystallizes in monoclinic system, space group P21/c with a = 6.8410(17), b = 20.123(5), c = 7.689(2) ?, β = 104.239(7)°, V = 1026.0(5) ?3, Z = 2, C16 H22 CuN4 O8 S2, Mr = 526.03, μ = 1.322 mm-1, Dc = 1.703 g/cm3, F(000) = 542, GOOF = 1.214, R = 0.0975 and w R = 0.2324. According to X-ray analysis, PFC-31 shows one-dimensional chain constructed by Cu(Ⅱ) centers coordinating with two pyridine groups, two sulfonate groups and two DMF molecules. Owing to the orderly functionalized sulfonate group and Lewis metal sites, PFC-31 exhibits good catalytic capability for CO2 cycloaddition of epoxide under normal pressure and temperature.展开更多
The hydrogen-bonded organic framework(PFC-32),constructed by tetrahydroxyquinone(THQN)and diethylamine(DEA),was readily prepared via hydrothermal synthesis in DEF(N,N-diethylformamide).PFC-32 was characterized by PXRD...The hydrogen-bonded organic framework(PFC-32),constructed by tetrahydroxyquinone(THQN)and diethylamine(DEA),was readily prepared via hydrothermal synthesis in DEF(N,N-diethylformamide).PFC-32 was characterized by PXRD,IR,UV-Vis,TGA and photoluminescence(PL).Single crystal analysis reveals that PFC-32 shows a three-dimensional(3 D)framework,where the THQN anions are coplanar and separated by DEA cations.PFC-32 displays intrinsic photoluminescence property owing to the alleviation of the aggregation-caused quenching(ACQ)effect caused byπ-πstacking.展开更多
文摘构建脂肪特异性蛋白27(Fat-specific protein of 27,Fsp27)基因沉默载体,研究沉默Fsp27基因表达对3T3-L1细胞脂解的影响,并对其作用机制进行探究。采用RNAi技术,构建Fsp27基因真核干扰载体,下调Fsp27基因的表达。“鸡尾酒”法诱导3T3-L1前脂肪细胞分化为成熟脂肪细胞。脂质体转染脂肪细胞,油红O染色脂滴,酶法测定细胞中甘油及甘油三酯的含量。Western blot法检测细胞中Fsp27、HSL、ATGL和PPARγ的蛋白表达。Western blot结果显示:阳性sh-Fsp27干扰载体均能有效下调Fsp27的表达,且伴随细胞内ATGL和PPARγ的表达量升高(P<0.05),其中sh-Fsp27-2的沉默效果最好;酶学方法检测结果显示:阳性sh-Fsp27干扰组细胞中甘油三酯含量下降,甘油含量升高(P<0.05);油红O染色结果发现:空白对照组与阴性对照组均有大脂滴堆积,阳性sh-Fsp27组小脂滴分布广泛,未见明显的大脂滴。sh-Fsp27-2组基因沉默载体的沉默效果最好,Fsp27基因沉默可以加快3T3-L1细胞的脂解速率,其主要是通过抑制脂滴融合和增强ATGL酶的水解来完成对脂解的调控。
基金Supported by “Strategic Priority Research Program” of the Chinese Academy of Sciences(No.XDB20000000)the National Natural Science Foundation of China(No.21871267 and 21561009)Hainan University Youth Fund Project(hdkyxj201715)
文摘A novel hydrogen-bonded organic framework, HOF-TCPP(HOF = hydrogen bonded organic framework, TCPP = tetrakis(4-carboxyphenyl) porphyrin), has been synthesized via solvothermal reaction in ethylene glycol. Crystal structure was well determined by single-crystal X-ray diffraction and powder X-ray diffraction(PXRD). Topological analysis reveals that HOF-TCPP exhibits sql 2D layer and features 2D → 3D polycatenation. Fluorescence investigation shows that HOF-TCPP displays much higher photoluminescence(PL) intensity than the amorphous ligands TCPP, which can be ascribed to the crystalline structure and hydrogen bonds existing in the structure.
基金the National Natural Science Foundation of China (No. 21871267)。
文摘An interesting metal-organic framework(MOF) PFC-31 was synthesized by a simple solvothermal reaction. X-ray crystallography reveals that PFC-31 crystallizes in monoclinic system, space group P21/c with a = 6.8410(17), b = 20.123(5), c = 7.689(2) ?, β = 104.239(7)°, V = 1026.0(5) ?3, Z = 2, C16 H22 CuN4 O8 S2, Mr = 526.03, μ = 1.322 mm-1, Dc = 1.703 g/cm3, F(000) = 542, GOOF = 1.214, R = 0.0975 and w R = 0.2324. According to X-ray analysis, PFC-31 shows one-dimensional chain constructed by Cu(Ⅱ) centers coordinating with two pyridine groups, two sulfonate groups and two DMF molecules. Owing to the orderly functionalized sulfonate group and Lewis metal sites, PFC-31 exhibits good catalytic capability for CO2 cycloaddition of epoxide under normal pressure and temperature.
基金Supported by Fujian Young and Middle-aged Teachers’Educational Research Project(No.JT180842)。
文摘The hydrogen-bonded organic framework(PFC-32),constructed by tetrahydroxyquinone(THQN)and diethylamine(DEA),was readily prepared via hydrothermal synthesis in DEF(N,N-diethylformamide).PFC-32 was characterized by PXRD,IR,UV-Vis,TGA and photoluminescence(PL).Single crystal analysis reveals that PFC-32 shows a three-dimensional(3 D)framework,where the THQN anions are coplanar and separated by DEA cations.PFC-32 displays intrinsic photoluminescence property owing to the alleviation of the aggregation-caused quenching(ACQ)effect caused byπ-πstacking.
