Nicotine is a secondary substance synthesized in tobacco roots. In flue-cured tobacco planting, tobacco decapitation is an effective practice to promote nicotine biosynthesis by regulation of the redistribution of tot...Nicotine is a secondary substance synthesized in tobacco roots. In flue-cured tobacco planting, tobacco decapitation is an effective practice to promote nicotine biosynthesis by regulation of the redistribution of total nitrogen amounts. However, proteins relevant to nicotine synthesis in tobacco roots has not been identified and characterized yet. It is important to explore the regulation of nicotine biosynthesis in tobacco roots. To identify the proteins relevant to nicotine synthesis, the protein patterns in roots of flue-cured tobacco （cv. K326） before and after decapitation were analyzed. In the present study, the protein patterns in roots of flue-cured tobacco were analyzed by two-dimensional electrophoresis （2-DE）, and the differentially-expressed spots were identified by matrix assisted laser desorption ionization time of flight mass spectrometry （MALDI-TOF-MS）. Paired comparison of 2-DE maps revealed 26 spots of differentially-expressed proteins in roots before and after decapitation. Furthermore, nine differentially-expressed spots were identified. There were four proteins which were enzymes possibly involved in nicotine biosynthesis. In addition, the roles of the four enzymes in nicotine biosynthesis were discussed in a putative network. Our results would contribute to the understanding of the regulation pathway of nicotine biosynthesis and further to the molecular manipulation on the nicotine contents in flue-cured tobacco.展开更多
The relatively high stress probably leads to generation of a fractured or even instable area around a working coalface. Also, the generated weak area often evolves into an easy-infiltrating field of water/gas to great...The relatively high stress probably leads to generation of a fractured or even instable area around a working coalface. Also, the generated weak area often evolves into an easy-infiltrating field of water/gas to greatly increase probability of accident occurrence. To reveal the distribution of high stress around working faces, we put forward the mode-I-crack compression model. In this model, the goaf following a working face is regarded as a mode-I crack in an infinite plate, and the self-gravity of overlaying strata is transformed into an uniform pressure applied normal to the upper edge of the model crack. Solving this problem is based on the Westergaard complex stress function. For comparison, the software RFPA-2D is also employed to simulate the same mining problem, and furthermore extendedly to calculate the stress interference induced by the simultaneous advances of two different working faces. The results show that, the area close to a working face or the goaf tail has the maximum stress, and the stress is distributed directly proportional to the square root of the advance and inversely proportional to the square root of the distance to the working face. The simultaneous advances of two neighboring working faces in different horizontals can lead to extremely high resultant stress in an interference area.展开更多
基金Natural Science Foundation of Henan Province, China (0624050013)Inovation Foundation of Tobacco Profession Cultivation Key Laboratory,China (06 TCIF 006)
文摘Nicotine is a secondary substance synthesized in tobacco roots. In flue-cured tobacco planting, tobacco decapitation is an effective practice to promote nicotine biosynthesis by regulation of the redistribution of total nitrogen amounts. However, proteins relevant to nicotine synthesis in tobacco roots has not been identified and characterized yet. It is important to explore the regulation of nicotine biosynthesis in tobacco roots. To identify the proteins relevant to nicotine synthesis, the protein patterns in roots of flue-cured tobacco （cv. K326） before and after decapitation were analyzed. In the present study, the protein patterns in roots of flue-cured tobacco were analyzed by two-dimensional electrophoresis （2-DE）, and the differentially-expressed spots were identified by matrix assisted laser desorption ionization time of flight mass spectrometry （MALDI-TOF-MS）. Paired comparison of 2-DE maps revealed 26 spots of differentially-expressed proteins in roots before and after decapitation. Furthermore, nine differentially-expressed spots were identified. There were four proteins which were enzymes possibly involved in nicotine biosynthesis. In addition, the roles of the four enzymes in nicotine biosynthesis were discussed in a putative network. Our results would contribute to the understanding of the regulation pathway of nicotine biosynthesis and further to the molecular manipulation on the nicotine contents in flue-cured tobacco.
基金Projects 50774083 and 40811120546 supported by the National Natural Science Foundation of ChinaNCET-07-0803 by the Program for New Century Ex-cellent Talents in University 2005CB221502 by the National Basic Research Program of China
文摘The relatively high stress probably leads to generation of a fractured or even instable area around a working coalface. Also, the generated weak area often evolves into an easy-infiltrating field of water/gas to greatly increase probability of accident occurrence. To reveal the distribution of high stress around working faces, we put forward the mode-I-crack compression model. In this model, the goaf following a working face is regarded as a mode-I crack in an infinite plate, and the self-gravity of overlaying strata is transformed into an uniform pressure applied normal to the upper edge of the model crack. Solving this problem is based on the Westergaard complex stress function. For comparison, the software RFPA-2D is also employed to simulate the same mining problem, and furthermore extendedly to calculate the stress interference induced by the simultaneous advances of two different working faces. The results show that, the area close to a working face or the goaf tail has the maximum stress, and the stress is distributed directly proportional to the square root of the advance and inversely proportional to the square root of the distance to the working face. The simultaneous advances of two neighboring working faces in different horizontals can lead to extremely high resultant stress in an interference area.
