目的本研究利用糖尿病动物模型,在体外环境下,研究C1q肿瘤坏死因子相关蛋白9(C1q/Tumornecrosis factor related protein 9,CTRP9)对糖尿病血小板异常激活的作用,以及其可能的受体通路。方法将6-8周龄雄性C57BL/6J小鼠共64只,随机平均分...目的本研究利用糖尿病动物模型,在体外环境下,研究C1q肿瘤坏死因子相关蛋白9(C1q/Tumornecrosis factor related protein 9,CTRP9)对糖尿病血小板异常激活的作用,以及其可能的受体通路。方法将6-8周龄雄性C57BL/6J小鼠共64只,随机平均分为2组,分别给予正常饮食及高脂饮食12周,检测其体质量、血糖、血小板数、胰岛素水平、血清CTRP9水平,并计算其胰岛素抵抗指数(homeostasis modelassessment-insulin resistance,HOMA-IR)水平;麻醉后取全血测定在不同因素干预组中血小板聚集效应的变化。结果与正常饮食组相比,高脂饮食的小鼠体质量增加,血糖和胰岛素水平升高,HOMA-IR指数升高,CTRP9水平降低,血小板最大聚集率升高,聚集曲线下面积增加。在体外环境下,加入CTRP9后,可改善两组小鼠血小板异常聚集的现象;在加入N-钙黏蛋白(N-cadherin)抑制剂ADH-1后,可抑制CTRP9对血小板异常聚集的抑制作用;与未给予任何干预的小鼠血小板对比,只加入ADH-1的样本上述指标未发现明显差异。上述效应在高脂饮食及正常饮食的小鼠中均有发现。结论在体外环境中,CTRP9可抑制糖尿病小鼠血小板的异常激活;抑制N-cadherin后,血小板聚集功能未受影响,CTRP9的作用消失,由此推断:N-cadherin可能为CTRP9抑制血小板异常激活的受体。展开更多
In order to simulate the recovery of groundwater funnels under the condition of reducing groundwater abstraction, hydrogeological conditions of recoverability construction of Shijiazhuang groundwater funnel were analy...In order to simulate the recovery of groundwater funnels under the condition of reducing groundwater abstraction, hydrogeological conditions of recoverability construction of Shijiazhuang groundwater funnel were analyzed, and a numerical simulation method was used based on the change of various parameters. The results show that the groundwater flow model can accurately reflect groundwater hydrogeological characteristics, and can guarantee the reliability of groundwater restoration prediction. The research has set up four schemes for rural water-saving, planting structure adjustment, urban reducing abstraction and integrated method. The effect of four restoration schemes on the restoration of groundwater funnels was compared with water table variations of two observation well. Comparison results show that the level changes of plan Three and Four are different from the other two kinds of exploitation and the drop trend of water table in the funnel area is flat. So we can conclude that Plan Three and Four have significant effect on the groundwater funnel restoration of Shijiazhuang.展开更多
文摘目的本研究利用糖尿病动物模型,在体外环境下,研究C1q肿瘤坏死因子相关蛋白9(C1q/Tumornecrosis factor related protein 9,CTRP9)对糖尿病血小板异常激活的作用,以及其可能的受体通路。方法将6-8周龄雄性C57BL/6J小鼠共64只,随机平均分为2组,分别给予正常饮食及高脂饮食12周,检测其体质量、血糖、血小板数、胰岛素水平、血清CTRP9水平,并计算其胰岛素抵抗指数(homeostasis modelassessment-insulin resistance,HOMA-IR)水平;麻醉后取全血测定在不同因素干预组中血小板聚集效应的变化。结果与正常饮食组相比,高脂饮食的小鼠体质量增加,血糖和胰岛素水平升高,HOMA-IR指数升高,CTRP9水平降低,血小板最大聚集率升高,聚集曲线下面积增加。在体外环境下,加入CTRP9后,可改善两组小鼠血小板异常聚集的现象;在加入N-钙黏蛋白(N-cadherin)抑制剂ADH-1后,可抑制CTRP9对血小板异常聚集的抑制作用;与未给予任何干预的小鼠血小板对比,只加入ADH-1的样本上述指标未发现明显差异。上述效应在高脂饮食及正常饮食的小鼠中均有发现。结论在体外环境中,CTRP9可抑制糖尿病小鼠血小板的异常激活;抑制N-cadherin后,血小板聚集功能未受影响,CTRP9的作用消失,由此推断:N-cadherin可能为CTRP9抑制血小板异常激活的受体。
基金supported by Public Welfare Project of Ministry of Water Resources(201501008)Natural Science Foundation of Hebei Province(D2015504019)
文摘In order to simulate the recovery of groundwater funnels under the condition of reducing groundwater abstraction, hydrogeological conditions of recoverability construction of Shijiazhuang groundwater funnel were analyzed, and a numerical simulation method was used based on the change of various parameters. The results show that the groundwater flow model can accurately reflect groundwater hydrogeological characteristics, and can guarantee the reliability of groundwater restoration prediction. The research has set up four schemes for rural water-saving, planting structure adjustment, urban reducing abstraction and integrated method. The effect of four restoration schemes on the restoration of groundwater funnels was compared with water table variations of two observation well. Comparison results show that the level changes of plan Three and Four are different from the other two kinds of exploitation and the drop trend of water table in the funnel area is flat. So we can conclude that Plan Three and Four have significant effect on the groundwater funnel restoration of Shijiazhuang.
