Background In county-level tuberculosis （TB） dispensaries in China,the accurate diagnosis of sputum smear-negative pulmonary tuberculosis （SNPT） needs to be improved by developing and validating clinical and radio...Background In county-level tuberculosis （TB） dispensaries in China,the accurate diagnosis of sputum smear-negative pulmonary tuberculosis （SNPT） needs to be improved by developing and validating clinical and radiographic predictors.Methods The study was conducted simultaneously in three counties per province in Chongqing Municipality and Liaoning Province in China between May 2005 and May 2006.A total of 432 new SNPT patients who are HIV-negative and more than 15 years old diagnosed by expert panels in county-level TB dispensaries were recruited.Their sputum samples were collected for culture before anti-TB treatment,and the treatment outcomes （changes of X-rays） were followed up at the end of the 6th month.Results Of the 432 SNPT patients,sputum culture positive （9.7%） or culture negative with good changes of X-rays at the end of the 6th month （73.6%） was validated as SNPT.Four predictive variables were associated with validated SNPT in the multivariate logistic regression model：age ≤55 years old （odds ratio （OR） 5.66; 95% CI 2.69-11.91）,〉60 days of cough （OR 3.73; 95% CI 1.10-12.65）,≥10% of pulmonary consolidation in the lungs （OR 5.40; 95% CI 2.90-10.06）,and pulmonary consolidation in the upper lobe anterior segment （OR 3.00; 95% CI 1.57-5.72）.The area under the receiver operating characteristic curve of the model was 0.77 （95% CI 0.71-0.83）.Conclusion Four predictors of clinical and radiological characteristics that had a good diagnostic performance of SNPT deserve to be recommended as index indicators of SNPT diagnosis in county-level TB dispensaries in China.展开更多
Six new tirucallane-type triterpenoids(1-6),along with ten known triterpenoids,were isolated from methylene chloride extract of the resin of Boswellia carterii Birdw.By the application of the comprehensive spectroscop...Six new tirucallane-type triterpenoids(1-6),along with ten known triterpenoids,were isolated from methylene chloride extract of the resin of Boswellia carterii Birdw.By the application of the comprehensive spectroscopic data,the structures of the compounds were clarified.The experimental electronic circular dichroism spectra were compared with those calculated,which allowed to assign the absolute configurations.Compounds 5 and 6 possesed a 2,3-seco tirucallane-type triterpenoid skeleton,which were first reported.Their inhibitory activity against NO formation in LPS-activated BV-2 cells were evaluated.Compound 9 showed appreciable inhibitory effect,with an IC_(50)value of 7.58±0.87μmol·L^(-1).展开更多
文摘Background In county-level tuberculosis （TB） dispensaries in China,the accurate diagnosis of sputum smear-negative pulmonary tuberculosis （SNPT） needs to be improved by developing and validating clinical and radiographic predictors.Methods The study was conducted simultaneously in three counties per province in Chongqing Municipality and Liaoning Province in China between May 2005 and May 2006.A total of 432 new SNPT patients who are HIV-negative and more than 15 years old diagnosed by expert panels in county-level TB dispensaries were recruited.Their sputum samples were collected for culture before anti-TB treatment,and the treatment outcomes （changes of X-rays） were followed up at the end of the 6th month.Results Of the 432 SNPT patients,sputum culture positive （9.7%） or culture negative with good changes of X-rays at the end of the 6th month （73.6%） was validated as SNPT.Four predictive variables were associated with validated SNPT in the multivariate logistic regression model：age ≤55 years old （odds ratio （OR） 5.66; 95% CI 2.69-11.91）,〉60 days of cough （OR 3.73; 95% CI 1.10-12.65）,≥10% of pulmonary consolidation in the lungs （OR 5.40; 95% CI 2.90-10.06）,and pulmonary consolidation in the upper lobe anterior segment （OR 3.00; 95% CI 1.57-5.72）.The area under the receiver operating characteristic curve of the model was 0.77 （95% CI 0.71-0.83）.Conclusion Four predictors of clinical and radiological characteristics that had a good diagnostic performance of SNPT deserve to be recommended as index indicators of SNPT diagnosis in county-level TB dispensaries in China.
基金supported by the National Natural Science Foundation of China(No.81973465)the National Science and Technology Major Project of the Ministry of Science and Technology of China(No.2018ZX09735005)the Key Laboratory Basic Research Projects of the Department of Education in Liaoning Province,China(No.LZ2014044)。
文摘Six new tirucallane-type triterpenoids(1-6),along with ten known triterpenoids,were isolated from methylene chloride extract of the resin of Boswellia carterii Birdw.By the application of the comprehensive spectroscopic data,the structures of the compounds were clarified.The experimental electronic circular dichroism spectra were compared with those calculated,which allowed to assign the absolute configurations.Compounds 5 and 6 possesed a 2,3-seco tirucallane-type triterpenoid skeleton,which were first reported.Their inhibitory activity against NO formation in LPS-activated BV-2 cells were evaluated.Compound 9 showed appreciable inhibitory effect,with an IC_(50)value of 7.58±0.87μmol·L^(-1).
