Hepatic stellate cells,hepatic sinusoidal endothelial cells and hepatic sinusoidal capllarization are closely related to the occurrence and progression of hepatic fibrosis.The pathological activation of hepatic stella...Hepatic stellate cells,hepatic sinusoidal endothelial cells and hepatic sinusoidal capllarization are closely related to the occurrence and progression of hepatic fibrosis.The pathological activation of hepatic stellate cels is the central link of hepatic fibrosis,and hepatic sinusoidal capillarization also promotes the occurrence and development of liver diseases.In the course of hepatic fibrosis,there is always a mutually reinforcing relationship between the activation of hepatic stellate cells and the capillarization of hepatic sinusoids.This paper strives to find an effective way to intervene or even reverse this vicious cycle by deeply investigating the effect of hepatic stellate cells and hepatic sinusoidal capillarization on hepatic fibrosis and their mutual promotion,and provide a new idea for the treatment of hepatic fibrosis,which is of great significance for relieving and reversing hepatic fibrosis.展开更多
筛选高原雨点鸽与詹森鸽胸肌飞行能力差异的关键基因,为赛鸽选育奠定基础。选择性别相同、体况与日龄相近的高原雨点鸽、詹森鸽各3只,屠宰后取胸肌,进行转录组测序,筛选差异表达基因,对差异基因进行Gene Ontology(GO)分析、Kyoto Encycl...筛选高原雨点鸽与詹森鸽胸肌飞行能力差异的关键基因,为赛鸽选育奠定基础。选择性别相同、体况与日龄相近的高原雨点鸽、詹森鸽各3只,屠宰后取胸肌,进行转录组测序,筛选差异表达基因,对差异基因进行Gene Ontology(GO)分析、Kyoto Encyclopedia of Genes and Genomes(KEGG)分析与蛋白质互作网络分析。结果表明:高原雨点鸽与詹森鸽胸肌转录组比较共检测到75个显著差异基因,49个基因上调表达,26个基因下调表达;GO功能分析显示,差异基因主要富集在骨骼肌细胞分化、细胞代谢过程调节等条目;KEGG通路分析显示,差异基因显著富集在胰岛素信号通路、AMPK信号通路等。与詹森鸽相比,高原雨点鸽胸肌SMYD1、STAT1、VEGFA、PPM1K、PLCE1基因上调,MYOD1、SOCS3、MGLL基因下调。STAT1、MYOD1可能导致高原雨点鸽胸肌生长慢于詹森鸽;SOCS3可能导致高原雨点鸽胸肌肌纤维直径变小,爆发力下降;PLCE1可能导致高原雨点鸽胸肌肌纤维增多;SMYD1可能导致肌纤维分化形成更多的红肌纤维;VEGFA可能导致高原雨点鸽体内白色脂肪转化为棕色脂肪;PPM1K、MGLL可能催化支链氨基酸分解,为高原雨点鸽长距离负重飞行提供足够能量,更适宜远距离飞行。展开更多
基金Supported by National Natural Science Foundation of China(81960761,82060825)Guangxi Natural Science Foundation(2020GXNSFAA297119)+2 种基金Guangxi First-class Discipline of Traditional Chinese Medicine(GJKY[2022]1)Guangxi Famous TCM Doctor Linjang Inheritance Studio(GZYYKJF[2021]6)Guangxi Postgraduate Education Innovation Program(YCSY2023004,YCSZ2022002).
文摘Hepatic stellate cells,hepatic sinusoidal endothelial cells and hepatic sinusoidal capllarization are closely related to the occurrence and progression of hepatic fibrosis.The pathological activation of hepatic stellate cels is the central link of hepatic fibrosis,and hepatic sinusoidal capillarization also promotes the occurrence and development of liver diseases.In the course of hepatic fibrosis,there is always a mutually reinforcing relationship between the activation of hepatic stellate cells and the capillarization of hepatic sinusoids.This paper strives to find an effective way to intervene or even reverse this vicious cycle by deeply investigating the effect of hepatic stellate cells and hepatic sinusoidal capillarization on hepatic fibrosis and their mutual promotion,and provide a new idea for the treatment of hepatic fibrosis,which is of great significance for relieving and reversing hepatic fibrosis.
文摘筛选高原雨点鸽与詹森鸽胸肌飞行能力差异的关键基因,为赛鸽选育奠定基础。选择性别相同、体况与日龄相近的高原雨点鸽、詹森鸽各3只,屠宰后取胸肌,进行转录组测序,筛选差异表达基因,对差异基因进行Gene Ontology(GO)分析、Kyoto Encyclopedia of Genes and Genomes(KEGG)分析与蛋白质互作网络分析。结果表明:高原雨点鸽与詹森鸽胸肌转录组比较共检测到75个显著差异基因,49个基因上调表达,26个基因下调表达;GO功能分析显示,差异基因主要富集在骨骼肌细胞分化、细胞代谢过程调节等条目;KEGG通路分析显示,差异基因显著富集在胰岛素信号通路、AMPK信号通路等。与詹森鸽相比,高原雨点鸽胸肌SMYD1、STAT1、VEGFA、PPM1K、PLCE1基因上调,MYOD1、SOCS3、MGLL基因下调。STAT1、MYOD1可能导致高原雨点鸽胸肌生长慢于詹森鸽;SOCS3可能导致高原雨点鸽胸肌肌纤维直径变小,爆发力下降;PLCE1可能导致高原雨点鸽胸肌肌纤维增多;SMYD1可能导致肌纤维分化形成更多的红肌纤维;VEGFA可能导致高原雨点鸽体内白色脂肪转化为棕色脂肪;PPM1K、MGLL可能催化支链氨基酸分解,为高原雨点鸽长距离负重飞行提供足够能量,更适宜远距离飞行。
