以Web of Science核心数据库中2000~2019年的1377篇草地恢复相关文献作为研究对象,使用CiteSpace 5.3.R11软件对其进行可视化分析,探讨草地恢复的研究态势及热点前沿。结果表明:(1)近二十年来,草地恢复研究领域的发文数量呈现波动增长...以Web of Science核心数据库中2000~2019年的1377篇草地恢复相关文献作为研究对象,使用CiteSpace 5.3.R11软件对其进行可视化分析,探讨草地恢复的研究态势及热点前沿。结果表明:(1)近二十年来,草地恢复研究领域的发文数量呈现波动增长的趋势,其中,《Restoration Ecology》是相关产量最高的期刊,而生态学是最受欢迎的学科类别;(2)发文前五位的国家为美国(35.44%)、中国(19.03%)、德国(7.04%)、英国(6.61%)和荷兰(5.95%),相关研究力量主要集中在高等教育研究机构,中国科学院是进行此类研究的关键机构之一;(3)在研究样本中,有12篇论文在该领域的发展中发挥了关键作用,为今后的研究奠定了坚实的基础;(4)关键词聚类分析表明,草地恢复领域的研究热点领域可归纳为:土壤种子库;湿地;生态恢复;草地管理;碳汇。同时,研究前沿分析表明,植物的功能多样性与生态系统服务功能、土地利用变化对草地恢复的影响、草地恢复过程中微生物群落和草地恢复方法对气候变化的适应性研究是该领域研究的新趋势。展开更多
As an important indicator of environmental and climate changes, snow chemical properties can be used to reflect microcosmic changes, large-scale environmental and climate changes. 174 groups of snow samples were colle...As an important indicator of environmental and climate changes, snow chemical properties can be used to reflect microcosmic changes, large-scale environmental and climate changes. 174 groups of snow samples were collected from four different rivers, Jinta river, Sishui river, Binggou river, and Nancha river, in the eastern Qilian Mountains in west China from May 2014 to October 2017. The characteristics of inorganic ions, Ca2+, Mg2+, Na+, K+, Cl–, NO3–, HCO3–, and SO2–, in the samples were analyzed by Dionex-600 and Dionex-3000 ion chromatograph. The results show that Ca2+ is the main cation, while HCO3– is the main anion;the ion concentration of snow is higher than that of rain. After careful analysis, we draw the conclusion that due to the controlling of the westerly wind, the atmosphere of the Qilian Mountains is dry with high dust content in winter and spring, which makes the ions in the snow mainly derive from the weathering of carbonate rock and sulfate rock. The ions in snow cover mainly come from land-sourced dust, while less contribution is from marine sources and human activities.展开更多
Citrus Huanglongbing(HLB, yellow shoot disease) is one of the most serious citrus diseases worldwide. To better improve the detection sensitivity, a droplet digital PCR(ddPCR) assay was developed for the rapid det...Citrus Huanglongbing(HLB, yellow shoot disease) is one of the most serious citrus diseases worldwide. To better improve the detection sensitivity, a droplet digital PCR(ddPCR) assay was developed for the rapid detection of ‘Candidatus Liberibacter asiaticus'(Las), the putative causal agent of HLB. The detection of sensitivity comparison using positive plasmid indicated that dd PCR was superior to quantitative PCR(qPCR) for detecting and quantifying Las at low concentrations. The Las detection of 40 field samples also showed that six of 13 asymptomatic samples(46.15%) with high Ct value(〉35) were positive by dd PCR. This methodology showed great potential for early HLB infection diagnosis.展开更多
文摘以Web of Science核心数据库中2000~2019年的1377篇草地恢复相关文献作为研究对象,使用CiteSpace 5.3.R11软件对其进行可视化分析,探讨草地恢复的研究态势及热点前沿。结果表明:(1)近二十年来,草地恢复研究领域的发文数量呈现波动增长的趋势,其中,《Restoration Ecology》是相关产量最高的期刊,而生态学是最受欢迎的学科类别;(2)发文前五位的国家为美国(35.44%)、中国(19.03%)、德国(7.04%)、英国(6.61%)和荷兰(5.95%),相关研究力量主要集中在高等教育研究机构,中国科学院是进行此类研究的关键机构之一;(3)在研究样本中,有12篇论文在该领域的发展中发挥了关键作用,为今后的研究奠定了坚实的基础;(4)关键词聚类分析表明,草地恢复领域的研究热点领域可归纳为:土壤种子库;湿地;生态恢复;草地管理;碳汇。同时,研究前沿分析表明,植物的功能多样性与生态系统服务功能、土地利用变化对草地恢复的影响、草地恢复过程中微生物群落和草地恢复方法对气候变化的适应性研究是该领域研究的新趋势。
基金funded by the National Natural Science Foundation of China (4166100541867030+1 种基金4197103641761047)
文摘As an important indicator of environmental and climate changes, snow chemical properties can be used to reflect microcosmic changes, large-scale environmental and climate changes. 174 groups of snow samples were collected from four different rivers, Jinta river, Sishui river, Binggou river, and Nancha river, in the eastern Qilian Mountains in west China from May 2014 to October 2017. The characteristics of inorganic ions, Ca2+, Mg2+, Na+, K+, Cl–, NO3–, HCO3–, and SO2–, in the samples were analyzed by Dionex-600 and Dionex-3000 ion chromatograph. The results show that Ca2+ is the main cation, while HCO3– is the main anion;the ion concentration of snow is higher than that of rain. After careful analysis, we draw the conclusion that due to the controlling of the westerly wind, the atmosphere of the Qilian Mountains is dry with high dust content in winter and spring, which makes the ions in the snow mainly derive from the weathering of carbonate rock and sulfate rock. The ions in snow cover mainly come from land-sourced dust, while less contribution is from marine sources and human activities.
基金funded by the National Natural Sciences Foundation of China (31671992, 31301635)the Chongqing Science and Technology Commission Project, China (cstc2016shms-ztzx80003)the Guangxi Key Laboratory of Citrus Biology, Guangxi Academy of Specialty Crops, China (SYS2015K004)
文摘Citrus Huanglongbing(HLB, yellow shoot disease) is one of the most serious citrus diseases worldwide. To better improve the detection sensitivity, a droplet digital PCR(ddPCR) assay was developed for the rapid detection of ‘Candidatus Liberibacter asiaticus'(Las), the putative causal agent of HLB. The detection of sensitivity comparison using positive plasmid indicated that dd PCR was superior to quantitative PCR(qPCR) for detecting and quantifying Las at low concentrations. The Las detection of 40 field samples also showed that six of 13 asymptomatic samples(46.15%) with high Ct value(〉35) were positive by dd PCR. This methodology showed great potential for early HLB infection diagnosis.