Soybean （Glycine max L. Merr.） is the world＇s foremost source of edible plant oil and proteins, meantime, the biologically active secondary metabolites such as saponins and isoflavones are benefit to human health. ...Soybean （Glycine max L. Merr.） is the world＇s foremost source of edible plant oil and proteins, meantime, the biologically active secondary metabolites such as saponins and isoflavones are benefit to human health. The objective of this study was to identify quantitative trait loci （QTL） and epistatic interactions associated with isoflavone, protein, and oil contents in soybean seeds. An F13 recombinant inbred line （RIL） comprising 474 lines was derived from a cross between Jindou 23 and Huibuzhi cultivars. SSR technique was employed for mapping of the QTLs. The QTLs for isoflavone, protein, and oil contents were analyzed and 23 QTLs were detected based on the constructed linkage map. Six QTLs for isoflavone content were localized in linkage groups J, N, D2, and G, eleven QTLs for oil content were localized in the linkage groups A1, A2, B2, C2, and D2, and six QTLs for protein content were localized in linkage groups B2, C2, G, and H1. The correlative analysis demonstrated that the isoflavone content had significant correlation with protein content, while significantly negative correlations was existed between oil and protein content, and significantly positive correlations was existed between protein and oil content. All these findings have laid an important basis for the marker assisted breeding in soybean. The phenotypic correlations of quantitative traits may be resulted from the correlation of the QTL controlling those traits.展开更多
年检过程中发现渤海油田"渤海友谊号"浮式生产储油卸油装置(Floating Production Storage and Offloading,FPSO)单点系泊系统单点左右舷系泊腿存在故障,为消除油田安全隐患,需更换系泊腿。通过研究系泊腿的结构形式,初步判断...年检过程中发现渤海油田"渤海友谊号"浮式生产储油卸油装置(Floating Production Storage and Offloading,FPSO)单点系泊系统单点左右舷系泊腿存在故障,为消除油田安全隐患,需更换系泊腿。通过研究系泊腿的结构形式,初步判断故障原因,确定只更换系泊腿上半部分的工作范围;在方案可行性研究阶段,对比分析停产工期、风险及施工效率等,制定在码头更换系泊腿的基本策略;在设计系泊腿拆卸方案时,选择重型销轴开销器作为首选、金刚石绳锯机作为备用的施工方案,最终成功完成单点系泊腿的快速更换工作,使得油田停产时间比原计划缩短18d,经济效益显著提高。该项目是解决水上软刚臂单点系泊腿故障问题的一个典型案例,涉及策略制定、施工方案设计、海上施工与油田复产、冬季施工气候窗口选择及主线辅线施工相互配合等,对类似工程的研究具有借鉴意义。展开更多
基金supported by the National Key Project for Transgenic Organisms Breeding,China(2008ZX08004-005,2009ZX08004-001B)the National 948 Project of China(2009-Z34,2010-G23)+3 种基金the National 973 Program of China(2008CB117005)the Henan Province key Scientific and Technological Project,China(102101110100)the Transformational Fund of Agricultural Scientific and Technological Achievements in China(2008D00010209)and the National Key Technologies R&D Program of China(2006BAD521B01-3)
文摘Soybean （Glycine max L. Merr.） is the world＇s foremost source of edible plant oil and proteins, meantime, the biologically active secondary metabolites such as saponins and isoflavones are benefit to human health. The objective of this study was to identify quantitative trait loci （QTL） and epistatic interactions associated with isoflavone, protein, and oil contents in soybean seeds. An F13 recombinant inbred line （RIL） comprising 474 lines was derived from a cross between Jindou 23 and Huibuzhi cultivars. SSR technique was employed for mapping of the QTLs. The QTLs for isoflavone, protein, and oil contents were analyzed and 23 QTLs were detected based on the constructed linkage map. Six QTLs for isoflavone content were localized in linkage groups J, N, D2, and G, eleven QTLs for oil content were localized in the linkage groups A1, A2, B2, C2, and D2, and six QTLs for protein content were localized in linkage groups B2, C2, G, and H1. The correlative analysis demonstrated that the isoflavone content had significant correlation with protein content, while significantly negative correlations was existed between oil and protein content, and significantly positive correlations was existed between protein and oil content. All these findings have laid an important basis for the marker assisted breeding in soybean. The phenotypic correlations of quantitative traits may be resulted from the correlation of the QTL controlling those traits.
文摘年检过程中发现渤海油田"渤海友谊号"浮式生产储油卸油装置(Floating Production Storage and Offloading,FPSO)单点系泊系统单点左右舷系泊腿存在故障,为消除油田安全隐患,需更换系泊腿。通过研究系泊腿的结构形式,初步判断故障原因,确定只更换系泊腿上半部分的工作范围;在方案可行性研究阶段,对比分析停产工期、风险及施工效率等,制定在码头更换系泊腿的基本策略;在设计系泊腿拆卸方案时,选择重型销轴开销器作为首选、金刚石绳锯机作为备用的施工方案,最终成功完成单点系泊腿的快速更换工作,使得油田停产时间比原计划缩短18d,经济效益显著提高。该项目是解决水上软刚臂单点系泊腿故障问题的一个典型案例,涉及策略制定、施工方案设计、海上施工与油田复产、冬季施工气候窗口选择及主线辅线施工相互配合等,对类似工程的研究具有借鉴意义。
