目的通过危害分析及关键控制点(hazard analysis and critical control point,HACCP)体系系统化控制核酸检测实验室质量,以期能够实现核酸检测实验室质量的提升,为有效制定核酸检测质量控制对策提供借鉴。方法根据HACCP体系初步探究核...目的通过危害分析及关键控制点(hazard analysis and critical control point,HACCP)体系系统化控制核酸检测实验室质量,以期能够实现核酸检测实验室质量的提升,为有效制定核酸检测质量控制对策提供借鉴。方法根据HACCP体系初步探究核酸检测实验室质量控制,重点分析HACCP体系在核酸标本接收、准备试剂、提取标本核酸、核酸扩增、结果判读、核酸检测结果报告环节制定的监控程序、验证措施以及纠偏措施等重要控制点。结果通过分析2021年12月-2022年12月的检测数据,结果显示,各批次核酸检测阳性质控品均满足判定规则,每一批次的实验均处于在控状态。对HACCP体系下实验室数据进行统计,计算其标准差、均值以及变异系数,对箱型图绘制后,展开离群值检验,结果发现,均值没有产生离群值。结论核酸检测实验室中应用HACCP体系,能够有效控制核酸检测实验室质量,全面掌控核酸检测实验室重要控制点,实现实验室生物安全水平与质量的提升,保证核酸检测实验室质控的有效性与科学性,继而有效控制核酸检测实验室质量。展开更多
Soybean Dongnong 42 from northeast of China was used as an experimental material.The samples were tested by spraying different concentrations of LaCl3,CeCl3 and LaCl3+CeCl3 solutions on the leaves at the seedling stag...Soybean Dongnong 42 from northeast of China was used as an experimental material.The samples were tested by spraying different concentrations of LaCl3,CeCl3 and LaCl3+CeCl3 solutions on the leaves at the seedling stage and the early flowering stage of the soybeans.Gas chromatography was used to determine the fatty acid content and ratio of the soybean seeds at the maturity stage.The fatty acid ratio of soybean seeds were evaluated by calculating saturated fatty acid(SFA),monounsaturated fatty acid(MUFA),polyunsaturated fatty acid(PUFA),unsaturated fatty acids(UFA),UFA/SFA,index unsaturated fatty acid(IUFA)and double bond index(DBI).(MUFA+PUFA)/SFA was used to study the effects of rare earths on the quality of soybean seeds.The results showed that spraying the rare earth solution could significantly optimize the fatty acid distribution ratio of soybean seeds.Compared with the control group(CK),stearic acid was reduced by 5%and linolenic acid was reduced by 3.72%.Except for La10,the oleic acid content increased significantly by 2.22%.Under the treatment of 30 mg·L^(−1) CeCl3,UFA,UFA/SFA and DBI were 83.56,5.08 and 9.47,respectively,which were the highest in each group.Under the treatment of 150 mg·L^(−1) LaCl3,(MUFA+PUFA)/SFA was 4.89,the closest meal to the recommended ratio.The above results showed that spraying appropriate concentrations of rare earth at the seedling stage and the early flowering stage could optimize the fatty acid ratio of soybean seeds and improve dietary fatty acid ratios.展开更多
The construction of the new urban district is one of the main strategies to extend the urban space in China. In recent years, with the performance of the national balanced development strategy of the regional economic...The construction of the new urban district is one of the main strategies to extend the urban space in China. In recent years, with the performance of the national balanced development strategy of the regional economics, the inter-administrative regional construction of the new urban district has been a tendency aiming to remove the administrative division barrier and facilitate the regional economic development. However, problems also arise in the new urban district under the background of the inter-administrative region such as the predicament caused by the ubiquitous regional economic cooperation and competition as well as the public governance, the lack of the management main body qualifi cation and the monotonous means of coordination. Referring to the experiences of the US in the inter-administrative region administration in the metropolitan area, the author is to put forward countermeasures and suggestions from such two aspects as the management system and the working mechanism.展开更多
文摘目的通过危害分析及关键控制点(hazard analysis and critical control point,HACCP)体系系统化控制核酸检测实验室质量,以期能够实现核酸检测实验室质量的提升,为有效制定核酸检测质量控制对策提供借鉴。方法根据HACCP体系初步探究核酸检测实验室质量控制,重点分析HACCP体系在核酸标本接收、准备试剂、提取标本核酸、核酸扩增、结果判读、核酸检测结果报告环节制定的监控程序、验证措施以及纠偏措施等重要控制点。结果通过分析2021年12月-2022年12月的检测数据,结果显示,各批次核酸检测阳性质控品均满足判定规则,每一批次的实验均处于在控状态。对HACCP体系下实验室数据进行统计,计算其标准差、均值以及变异系数,对箱型图绘制后,展开离群值检验,结果发现,均值没有产生离群值。结论核酸检测实验室中应用HACCP体系,能够有效控制核酸检测实验室质量,全面掌控核酸检测实验室重要控制点,实现实验室生物安全水平与质量的提升,保证核酸检测实验室质控的有效性与科学性,继而有效控制核酸检测实验室质量。
基金Supported by the National Natural Science Foundation of China(31471440)。
文摘Soybean Dongnong 42 from northeast of China was used as an experimental material.The samples were tested by spraying different concentrations of LaCl3,CeCl3 and LaCl3+CeCl3 solutions on the leaves at the seedling stage and the early flowering stage of the soybeans.Gas chromatography was used to determine the fatty acid content and ratio of the soybean seeds at the maturity stage.The fatty acid ratio of soybean seeds were evaluated by calculating saturated fatty acid(SFA),monounsaturated fatty acid(MUFA),polyunsaturated fatty acid(PUFA),unsaturated fatty acids(UFA),UFA/SFA,index unsaturated fatty acid(IUFA)and double bond index(DBI).(MUFA+PUFA)/SFA was used to study the effects of rare earths on the quality of soybean seeds.The results showed that spraying the rare earth solution could significantly optimize the fatty acid distribution ratio of soybean seeds.Compared with the control group(CK),stearic acid was reduced by 5%and linolenic acid was reduced by 3.72%.Except for La10,the oleic acid content increased significantly by 2.22%.Under the treatment of 30 mg·L^(−1) CeCl3,UFA,UFA/SFA and DBI were 83.56,5.08 and 9.47,respectively,which were the highest in each group.Under the treatment of 150 mg·L^(−1) LaCl3,(MUFA+PUFA)/SFA was 4.89,the closest meal to the recommended ratio.The above results showed that spraying appropriate concentrations of rare earth at the seedling stage and the early flowering stage could optimize the fatty acid ratio of soybean seeds and improve dietary fatty acid ratios.
基金supported by the Soft Science Project of Guizhou Province(Grant No.LH[2014]7276)the Development Research Project of Gui-An New District of 2014(Grant No.GAZ2014-01)
文摘The construction of the new urban district is one of the main strategies to extend the urban space in China. In recent years, with the performance of the national balanced development strategy of the regional economics, the inter-administrative regional construction of the new urban district has been a tendency aiming to remove the administrative division barrier and facilitate the regional economic development. However, problems also arise in the new urban district under the background of the inter-administrative region such as the predicament caused by the ubiquitous regional economic cooperation and competition as well as the public governance, the lack of the management main body qualifi cation and the monotonous means of coordination. Referring to the experiences of the US in the inter-administrative region administration in the metropolitan area, the author is to put forward countermeasures and suggestions from such two aspects as the management system and the working mechanism.
