以ZIF-8为固定化载体,通过原位合成法固定褶皱假丝酵母脂肪酶(CRL),研究了不同固定化条件对固定化酶活力的影响以及固定化酶的稳定性,并对固定化酶进行了结构表征。结果表明:在10 m L 40 mmol/L硝酸锌溶液、10 m L 160 mmol/L 2-甲基咪...以ZIF-8为固定化载体,通过原位合成法固定褶皱假丝酵母脂肪酶(CRL),研究了不同固定化条件对固定化酶活力的影响以及固定化酶的稳定性,并对固定化酶进行了结构表征。结果表明:在10 m L 40 mmol/L硝酸锌溶液、10 m L 160 mmol/L 2-甲基咪唑溶液、2-甲基咪唑溶液初始p H8、酶添加量2.75 mg、-48℃冷冻干燥条件下,获得的固定化酶CRL@ZIF-8蛋白回收率为93%,比活力为1.08 U/mg;底物的分子大小对固定化酶的催化性能有明显影响,在以相对小分子的乙酸对硝基苯酯为底物时,重复使用9次后固定化酶仍保持72.8%的相对活力;通过对固定化酶进行场发射扫描电镜和氮气吸附脱附表征,表明脂肪酶CRL在载体表面和内部均有分布,固定化酶颗粒粒径约400 nm。展开更多
An one-dimension al three-tile quasilattice model is introduced.A multifractal spectral behavior has been found analytically and confirmed by the numerical simulation.The renormalixaction-group method has been used to...An one-dimension al three-tile quasilattice model is introduced.A multifractal spectral behavior has been found analytically and confirmed by the numerical simulation.The renormalixaction-group method has been used to study the asymptotical behavior of the spectrum,a pseudo-seven-cycle of trace map is found.展开更多
The scaling and the scaling-relevant spectral properties of Penrose tiling are investigated.The fractal dimension df of this tiling is analytically obtained,which is two,equal to its Euclidean dimension.Similar to usu...The scaling and the scaling-relevant spectral properties of Penrose tiling are investigated.The fractal dimension df of this tiling is analytically obtained,which is two,equal to its Euclidean dimension.Similar to usual self-similar structure,the vibrational density of states for Penrose lattice is also found to follow a power law p(ω)~ωds^(-1) with spectral dimension ds=2,which accounts for a special vibrational excitation in quasicrystals:the fracton-like excitation,whose state is critical.The simulation of random walk on this Penrose lattice indicates that the diffusive dimension d_(w)=2,thus the relation ds=2df/d_(w) holds.展开更多
Negative eigenvalue counting method has been extended to deal with the closed ring with large number of atoms threaded by a magnetic flux.This new method is extremely computer memory-saving and can be used to accurate...Negative eigenvalue counting method has been extended to deal with the closed ring with large number of atoms threaded by a magnetic flux.This new method is extremely computer memory-saving and can be used to accurately determine the eigen-values and eigenvectors.As numerical examples,the results of second moment and persistent current are presented.展开更多
By using the Hardy-Wright-Mobius inverse formula,we have improved the Carlsson-Gelatt-Ehrenreich scheme for the inverse problem of cohesive energy.The advantage of the new method is that the inverse formula are in a s...By using the Hardy-Wright-Mobius inverse formula,we have improved the Carlsson-Gelatt-Ehrenreich scheme for the inverse problem of cohesive energy.The advantage of the new method is that the inverse formula are in a sense independent of the geometries of lattices.展开更多
A new class of 1D quasiperiodic lattices,for which the substitution rules are B→BA,and A→BAB,has been studied in several aspects.The high-dimensional projection method for obtaining the quasilattice is presented.A m...A new class of 1D quasiperiodic lattices,for which the substitution rules are B→BA,and A→BAB,has been studied in several aspects.The high-dimensional projection method for obtaining the quasilattice is presented.A multifractral spectral behavior and gap fabeling properties have been found,which display the perfect quasiperiodicity of the studied model.展开更多
文摘以ZIF-8为固定化载体,通过原位合成法固定褶皱假丝酵母脂肪酶(CRL),研究了不同固定化条件对固定化酶活力的影响以及固定化酶的稳定性,并对固定化酶进行了结构表征。结果表明:在10 m L 40 mmol/L硝酸锌溶液、10 m L 160 mmol/L 2-甲基咪唑溶液、2-甲基咪唑溶液初始p H8、酶添加量2.75 mg、-48℃冷冻干燥条件下,获得的固定化酶CRL@ZIF-8蛋白回收率为93%,比活力为1.08 U/mg;底物的分子大小对固定化酶的催化性能有明显影响,在以相对小分子的乙酸对硝基苯酯为底物时,重复使用9次后固定化酶仍保持72.8%的相对活力;通过对固定化酶进行场发射扫描电镜和氮气吸附脱附表征,表明脂肪酶CRL在载体表面和内部均有分布,固定化酶颗粒粒径约400 nm。
基金Supported by the National Natural Science Foundation of China.
文摘An one-dimension al three-tile quasilattice model is introduced.A multifractal spectral behavior has been found analytically and confirmed by the numerical simulation.The renormalixaction-group method has been used to study the asymptotical behavior of the spectrum,a pseudo-seven-cycle of trace map is found.
基金Supported by the National Education Foundation of China for Ph.D.Natural Science Foundation of South China University of Technology.
文摘The scaling and the scaling-relevant spectral properties of Penrose tiling are investigated.The fractal dimension df of this tiling is analytically obtained,which is two,equal to its Euclidean dimension.Similar to usual self-similar structure,the vibrational density of states for Penrose lattice is also found to follow a power law p(ω)~ωds^(-1) with spectral dimension ds=2,which accounts for a special vibrational excitation in quasicrystals:the fracton-like excitation,whose state is critical.The simulation of random walk on this Penrose lattice indicates that the diffusive dimension d_(w)=2,thus the relation ds=2df/d_(w) holds.
文摘Negative eigenvalue counting method has been extended to deal with the closed ring with large number of atoms threaded by a magnetic flux.This new method is extremely computer memory-saving and can be used to accurately determine the eigen-values and eigenvectors.As numerical examples,the results of second moment and persistent current are presented.
基金Supported by the National Natural Science Foundation of China,and in part by the International Program in the Physical Sciences,Uppsala University,Sweden.
文摘By using the Hardy-Wright-Mobius inverse formula,we have improved the Carlsson-Gelatt-Ehrenreich scheme for the inverse problem of cohesive energy.The advantage of the new method is that the inverse formula are in a sense independent of the geometries of lattices.
基金Supported by the National Natural Science Foundation of China。
文摘A new class of 1D quasiperiodic lattices,for which the substitution rules are B→BA,and A→BAB,has been studied in several aspects.The high-dimensional projection method for obtaining the quasilattice is presented.A multifractral spectral behavior and gap fabeling properties have been found,which display the perfect quasiperiodicity of the studied model.
