Isolation and Characterization of Penicillium oxalicum ZHJ6 for Biodegradation of Methamidophos

One methamidophos-degrading fungus strain, named as ZHJ6, was isolated from the soils contaminated with methamidophos. It was identified as Penicillium oxalicum based on its morphological characteristics and ITS rDNA gene sequence analysis. The effects of carbon source, nitrogen source and the concentration of methamidophos, temperature and pH on the degradation were investigated. The results showed that the strain could use glucose as carbon source and the methamidophos as sole nitrogen source. The degradation ratio of methamidophos, when the initial concentration was 1.0× 10^-3 mg mL^-1, could reach above 99.9% in 12 incubation days. The strain could use ethanol, glucose, fructose, sucrose, lactose, starch, and dextrin as its carbon and energy source to degrade the methamidophos. The favorable degrading condition of the strain ZHJ6 was in a mineral salt medium at pH 5.0 and 25℃ with 1% glucose, and further studies showed that the strain could degrade folimat, phoxim and glyphosate with glucose as carbon source, but could not degrade chlorpyrifos, phosdrin, trichlorphon, and dichlorvos.

基于文化转译的桃花坞木版年画数字文创设计

目的研究国家级非遗桃花坞木版年画在数字文创产品中的创新应用。针对互联网的传播与时代审美需求,探索传统文化的活化路径。方法运用“文化转译”方法展开桃花坞木版年画文化IP的提取,进行文化IP物质层、行为层、精神层的设计转译。在获得视觉要素筛选结果、数字文创APP信息架构、意义体验达成路径以后,采用“APP+IP形象”的思路,进行数字文创设计开发。结果围绕文化IP,完成APP用户体验设计、角色形象设计、表情包设计与引导页设计。结论“文化转译”打造文化IP并生成数字文创的方法,融通了传统与现实,有助于深度挖掘非遗类数字文创的文化主题,保证数字文创产品文化层次结构的完整性,是传统文化创新式传承的新思路。

高校师德状况调查分析及师德建设对策研究

本文通过调研活动的方式,详细列举了高校师德建设存在的问题,深刻挖掘了问题存在的原因和根源,并围绕如何提高师德水平提出了若干建设性的建议和对策,对于促进高校师德建设具有重要作用。

Characterization of a bioflocculant from a newly isolated Vagococcus sp. W31

Screening of microorganisms producing flocculating substances was carried out. A strain secreting a large amount of bioflocculant was isolated from wastewater samples collected from the Little Moon River in Beijing. Based on the morphological properties and 16S rDNA sequence analysis, the isolate (designated W31) was classified as Vagococcus sp. A bioflocculant (named MBFW31) produced by W31 was extracted from the culture broth by ethanol precipitation and purified by gel chroma-tography. MBFW31 was heat-stable and had strong flocculating activity in a wide range of pH with relatively low dosage re-quirement. MBFW31 was identified as a polysaccharide with molecular weight over 2×106. It contained neutral sugar and uronic acid as its major and minor components, respectively. Infrared spectra showed the presence of hydroxyl, carboxyl and methoxyl group in its molecules. The present results suggested that MBFW31 had potential application in wastewater treatment.

Polymerization of Norbornene via an Iron-Based Complex/MAO Catalytic System

Polynorbornenes were synthesized in the presence of an iron based catalyst, 2,6-bis[1-(2,6-diisopropyl-phenylimino)ethyl]pyridine iron(Ⅱ) dichloride. The FTIR, 1H NMR and 13C NMR analysis results revealed that the structure of the obtained polynorbornenes consisted of vinyl addition polymer substructures without any ring-opening structures. The polymers were amorphous with a short-range order, displayed in the WAXD(wide angle X-ray diffraction) diagrams. The glass transition temperatures ranged from 200 to 400 ℃. The effects of the polymerization reaction conditions, such as Al/Fe molar ratio and toluene/CH_2Cl_2 volume ratio, on the activity, intrinsic viscosity and T_g were also studied.

孕妇硒营养状态及其与妊娠结局的关系

微量元素硒在维持正常妊娠功能中发挥着重要的作用。对近年来孕妇硒营养状态的流行现况及不同孕期硒营养状态对妊娠结局的影响进行回顾和分析,以期为孕期硒营养的研究提供帮助。现有的研究大多阐明了妊娠期某一时间点的硒营养状态,尚未能全面反映妊娠期硒营养状态变化对妊娠结局的影响,妊娠期孕妇硒营养状态与妊娠结局的相关性还有待进一步深入研究。

糜子叶片内源激素及保护系酶对黑穗病菌胁迫的响应

为了探究不同品种糜子叶片的内源激素及保护系酶对黑穗病菌胁迫的响应,本实验以4个不同抗性水平的糜子品种为材料,测定了各材料各时期顶三叶的内源激素含量以及保护系酶的活性。结果表明,感染黑穗病菌后,各品种糜子叶片的生长素和赤霉素含量降低,细胞分裂素、脱落酸、水杨酸、茉莉酸和丙二醛含量增加,超氧化物歧化酶和过氧化物酶活性增加。大部分时期,抗病品种糜子叶片的细胞分裂素、水杨酸和JA含量均显著高于感病品种,而生长素、赤霉素和脱落酸含量在抗、感品种间无显著差异。抗病品种糜子叶片的过氧化物酶活性显著高于感病品种,而丙二醛含量显著低于感病品种。感病早期,抗病品种叶片超氧化物歧化酶活性显著高于感病品种,但在后期则显著低于感病品种。