Keratinocytes and fibroblasts,derived from hiPSCs,were used to construct the human epidermal model by a culture patch made by monolayer poly-(lactic-co-glycolic acid)(PLGA)nanofibers and a human skin-on-a-chip device....Keratinocytes and fibroblasts,derived from hiPSCs,were used to construct the human epidermal model by a culture patch made by monolayer poly-(lactic-co-glycolic acid)(PLGA)nanofibers and a human skin-on-a-chip device.Unlike the conventional culture dish method,two different epidermal cells are successfully adhered to the front and back sides of the patch,which produces a three-dimensional nanofibrous scaffold similar to a natural extracellular matrix before the patch was cultured in the skin-on-a-chip device to mimic the physiological conditions of human skin.As expected,the differentiated hiPSCs show the expression of keratinocyte-and fibroblast-specific proteins on the patch,and the layering is found between these two kinds of cells,indicating that this approach creates a powerful in vitro system for modeling skin development and diseases.展开更多
要:利用分子模拟平台Discovery Studio 4.5研究分析黄腐酸和三七中人参皂苷Rg1分别对蛋白磷酸酯酶2A(Protein phosphatase2A,PP2A)的影响。结果表明,人参皂苷Rg1通过促进PP2A的活性以减少tau蛋白的过度磷酸化,从而防治阿尔茨海默病(Alzh...要:利用分子模拟平台Discovery Studio 4.5研究分析黄腐酸和三七中人参皂苷Rg1分别对蛋白磷酸酯酶2A(Protein phosphatase2A,PP2A)的影响。结果表明,人参皂苷Rg1通过促进PP2A的活性以减少tau蛋白的过度磷酸化,从而防治阿尔茨海默病(Alzheimer disease,AD)。采用CDOCKER对接技术对黄腐酸和人参皂苷Rg1分别与PP2A结合研究表明,黄腐酸也可能与PP2A发生作用,减少tau蛋白的过度磷酸化。与人参皂苷Rg1和PP2A形成的复合物对接结果表明,黄腐酸可能促进人参皂苷Rg1与PP2A的结合,从而增强PP2A的药理活性。展开更多
基金by the National Natural Science Foundation of China(Nos.31600801 and 11804121)the Natural Science Foundation of Hubei(No.2019CFB558)the Central Government Guides the Special Fund Project of Local Scientific and Technological Development(No.[2016]109)。
文摘Keratinocytes and fibroblasts,derived from hiPSCs,were used to construct the human epidermal model by a culture patch made by monolayer poly-(lactic-co-glycolic acid)(PLGA)nanofibers and a human skin-on-a-chip device.Unlike the conventional culture dish method,two different epidermal cells are successfully adhered to the front and back sides of the patch,which produces a three-dimensional nanofibrous scaffold similar to a natural extracellular matrix before the patch was cultured in the skin-on-a-chip device to mimic the physiological conditions of human skin.As expected,the differentiated hiPSCs show the expression of keratinocyte-and fibroblast-specific proteins on the patch,and the layering is found between these two kinds of cells,indicating that this approach creates a powerful in vitro system for modeling skin development and diseases.
文摘要:利用分子模拟平台Discovery Studio 4.5研究分析黄腐酸和三七中人参皂苷Rg1分别对蛋白磷酸酯酶2A(Protein phosphatase2A,PP2A)的影响。结果表明,人参皂苷Rg1通过促进PP2A的活性以减少tau蛋白的过度磷酸化,从而防治阿尔茨海默病(Alzheimer disease,AD)。采用CDOCKER对接技术对黄腐酸和人参皂苷Rg1分别与PP2A结合研究表明,黄腐酸也可能与PP2A发生作用,减少tau蛋白的过度磷酸化。与人参皂苷Rg1和PP2A形成的复合物对接结果表明,黄腐酸可能促进人参皂苷Rg1与PP2A的结合,从而增强PP2A的药理活性。