Peptide nucleic acids(PNAs) are nucleic acidanalogs with the deoxyribose phosphate backbone replaced by pseudo-peptide polymers to which the nucleobases arelinked.The achiral,uncharged and rather flexible properties o...Peptide nucleic acids(PNAs) are nucleic acidanalogs with the deoxyribose phosphate backbone replaced by pseudo-peptide polymers to which the nucleobases arelinked.The achiral,uncharged and rather flexible properties of the peptide backbone permit peptide nucleic acids more potential than oligonucleotides in application to antisenceand antigenic reagents.The process of PNA binding to DNA duplex and forming triplex is the first step of PNA interact-ing with PNA.But there are no PNA.2DNA triplex crystaldata up to date and little has been reported on the structure features and the force of the PNA.2DNA triplex.In thiswork,PNA(T).DNA(AT) triplexes are successfully built and the structures and forces to stabilize the triplex after optimi-zations and molecule dynamics are systematically exam-ined,which are expected to aid in the application of PNAs as anticense and antigene agents.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.39770418 and 39570395)the Post Doctor Foundation.
文摘Peptide nucleic acids(PNAs) are nucleic acidanalogs with the deoxyribose phosphate backbone replaced by pseudo-peptide polymers to which the nucleobases arelinked.The achiral,uncharged and rather flexible properties of the peptide backbone permit peptide nucleic acids more potential than oligonucleotides in application to antisenceand antigenic reagents.The process of PNA binding to DNA duplex and forming triplex is the first step of PNA interact-ing with PNA.But there are no PNA.2DNA triplex crystaldata up to date and little has been reported on the structure features and the force of the PNA.2DNA triplex.In thiswork,PNA(T).DNA(AT) triplexes are successfully built and the structures and forces to stabilize the triplex after optimi-zations and molecule dynamics are systematically exam-ined,which are expected to aid in the application of PNAs as anticense and antigene agents.
