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Expression of Duck Interferon Alpha in BL21(DE3)plysS
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作者 RENGui-ping QUJuan-juan +4 位作者 PEIFu-cheng LIJing-peng liuxiang-yu LILu WANGJun-wei 《Journal of Northeast Agricultural University(English Edition)》 CAS 2005年第1期11-13,共3页
To obtain the protein of duck interferon alpha and study its biological activities, the prokaryotic expression vector of DuIFN-αwas constructed and expressed in BL21 (DE3) plysS. Using PCR technique, the protein gene... To obtain the protein of duck interferon alpha and study its biological activities, the prokaryotic expression vector of DuIFN-αwas constructed and expressed in BL21 (DE3) plysS. Using PCR technique, the protein gene of DuIFN-αwas cloned from pMD-18-duIFN-αrecombinant. The gene was then inserted to pGEM-T vector and identified by restriction endonuclease analysis and sequencing. DuIFN-αwas ligated with the prokaryotic expression vector of pET30 a, then transformed into BL21 (DE3) plysS. The best inducing time and IPTG concentration for the expression of this recombinant protein was tested through the expression of the positive recombinant with different time span and different IPTG concentration. Lots of the protein of DuIFN-αwere expressed in BL21(DE3)plysS with 1 mmol·L-1 IPTG for 4 hours and its molecular weight for 34 000. 展开更多
关键词 DUCK Α-INTERFERON clonging EXPRESSION BL21(DE3)plysS
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