目的:探讨下调生长阻滞和DNA损伤诱导蛋白45β(growth arrest and DNA damage inducible protein 45β,GADD45β)表达对PC9肺腺癌细胞及吉非替尼敏感性的影响。方法:设计并合成GADD45β基因小干扰RNA(GADD45β-small interfering RNA,GA...目的:探讨下调生长阻滞和DNA损伤诱导蛋白45β(growth arrest and DNA damage inducible protein 45β,GADD45β)表达对PC9肺腺癌细胞及吉非替尼敏感性的影响。方法:设计并合成GADD45β基因小干扰RNA(GADD45β-small interfering RNA,GADD45β-siRNA)序列,通过慢病毒介导将GADD45β-siRNA转入PC9肺腺癌细胞中,通过实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)和Western印迹检测转染前后PC9肺腺癌细胞GADD45β的mRNA及蛋白水平,采用膜联蛋白V(annexin V)-别藻蓝蛋白(allophycocyanin,APC)双染流式细胞法检测转染后细胞凋亡水平;通过流式细胞术检测转染后细胞内DNA含量,计算转染后细胞各周期时相百分率,分析转染对细胞生长周期的影响;通过计数克隆形成数检测RNA干扰对细胞成瘤能力的影响;采用MTT法检测PC9肺腺癌细胞的吉非替尼半数抑制浓度IC50。结果:筛选出5'-AAATCCACTTCACGCTCAT-3'为GADD45β基因RNA干扰的有效序列。转染GADD45β-siRNA 48 h后,qRT-PCR和Western印迹结果显示PC9肺腺癌细胞GADD45β的mRNA和蛋白表达水平明显下调(均P<0.05),细胞凋亡率明显增加(P<0.05),且成瘤克隆数明显减少(P<0.05);PC9肺腺癌细胞位于S期及G2/M期细胞增多(P<0.05),吉非替尼的IC50明显下降(P<0.05)。结论:PC9肺腺癌细胞转染GADD45β-siRNA后,能成功下调GADD45β基因的mRNA和蛋白表达;下调GADD45β表达可降低PC9肺腺癌细胞的克隆形成能力,促进细胞凋亡;下调GADD45β表达可明显提高PC9肺腺癌细胞对吉非替尼的敏感性。展开更多
In this paper, by using the theory of semigroup and spectrum, a computation formula on the growth order of one class of C0-semigroups in Banach space isproved.
An agrobacterium tumefaciens-mediated co-tr- ansformation method was established and successfully used to transfer an antisense waxy gene into rice. The binary vec- tor p13W4 contains an antisense waxy gene, a hygromy...An agrobacterium tumefaciens-mediated co-tr- ansformation method was established and successfully used to transfer an antisense waxy gene into rice. The binary vec- tor p13W4 contains an antisense waxy gene, a hygromycin resistance gene (hpt) and a report gene (gus). The other bi- nary vector p13W8 contains only an antisense waxy gene in the T-DNA region. Two separate strains of A. tumefaciens, containing p13W4 and p13W8 mixed at a ratio of 1︰9, were used to transform a high-yield rice strain Chao 2-10. A total of 34 transgenic plants were obtained. PCR analysis showed that 15 of them were co-transformed. After GUS staining, the T1seeds with GUS positive were eliminated. The GUS nega- tive T1 seeds were germinated, and the seedlings were ana- lyzed for the existence of antisense waxy gene. The transgenic T1 plants with only antisense waxy gene further were con- firmed through Southern blot analysis. The T2 seeds collected from marker-free T1 plants were used for amylose content analysis. The results showed that the amylose content in marker-free transgenic seeds reduced by up to 28.61% over the wild type rice. This marker-free transgenic rice with low amylose contents obtained in this project could be used to screen soft grain, and could also be used as a new rice re- source for eating quality improvement.展开更多
In multi-cell cooperative multi-input multi-output （MIMO） systems, base station （BS） can exchange and utilize channel state information （CSI） of adjacent cell users to manage co-channel interference. Users quant...In multi-cell cooperative multi-input multi-output （MIMO） systems, base station （BS） can exchange and utilize channel state information （CSI） of adjacent cell users to manage co-channel interference. Users quantize the CSIs of desired channel and interference channels using finite-rate feedback links, then BS can generate cooperative block diagonalization （BD） precoding matrices using the obtained quantized CSI at transmitter to supress co-channel interference. In this paper, a novel adaptive bit allocation scheme is proposed to minimize the rate loss due to imperfect CSI. We derive the closed-form expression of rate loss caused by both channel delay and limited feedback. Based on the derived rate loss expression, the proposed scheme can adaptively allocate more bits to quantize the better channels with smaller delays and fewer bits to worse channels with larger delays. Simulation results show that the proposed scheme yields higher performance than other allocation schemes.展开更多
文摘目的:探讨下调生长阻滞和DNA损伤诱导蛋白45β(growth arrest and DNA damage inducible protein 45β,GADD45β)表达对PC9肺腺癌细胞及吉非替尼敏感性的影响。方法:设计并合成GADD45β基因小干扰RNA(GADD45β-small interfering RNA,GADD45β-siRNA)序列,通过慢病毒介导将GADD45β-siRNA转入PC9肺腺癌细胞中,通过实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)和Western印迹检测转染前后PC9肺腺癌细胞GADD45β的mRNA及蛋白水平,采用膜联蛋白V(annexin V)-别藻蓝蛋白(allophycocyanin,APC)双染流式细胞法检测转染后细胞凋亡水平;通过流式细胞术检测转染后细胞内DNA含量,计算转染后细胞各周期时相百分率,分析转染对细胞生长周期的影响;通过计数克隆形成数检测RNA干扰对细胞成瘤能力的影响;采用MTT法检测PC9肺腺癌细胞的吉非替尼半数抑制浓度IC50。结果:筛选出5'-AAATCCACTTCACGCTCAT-3'为GADD45β基因RNA干扰的有效序列。转染GADD45β-siRNA 48 h后,qRT-PCR和Western印迹结果显示PC9肺腺癌细胞GADD45β的mRNA和蛋白表达水平明显下调(均P<0.05),细胞凋亡率明显增加(P<0.05),且成瘤克隆数明显减少(P<0.05);PC9肺腺癌细胞位于S期及G2/M期细胞增多(P<0.05),吉非替尼的IC50明显下降(P<0.05)。结论:PC9肺腺癌细胞转染GADD45β-siRNA后,能成功下调GADD45β基因的mRNA和蛋白表达;下调GADD45β表达可降低PC9肺腺癌细胞的克隆形成能力,促进细胞凋亡;下调GADD45β表达可明显提高PC9肺腺癌细胞对吉非替尼的敏感性。
文摘In this paper, by using the theory of semigroup and spectrum, a computation formula on the growth order of one class of C0-semigroups in Banach space isproved.
基金the Shanghai Science and Technology Development Fund(Grant No.023112027).
文摘An agrobacterium tumefaciens-mediated co-tr- ansformation method was established and successfully used to transfer an antisense waxy gene into rice. The binary vec- tor p13W4 contains an antisense waxy gene, a hygromycin resistance gene (hpt) and a report gene (gus). The other bi- nary vector p13W8 contains only an antisense waxy gene in the T-DNA region. Two separate strains of A. tumefaciens, containing p13W4 and p13W8 mixed at a ratio of 1︰9, were used to transform a high-yield rice strain Chao 2-10. A total of 34 transgenic plants were obtained. PCR analysis showed that 15 of them were co-transformed. After GUS staining, the T1seeds with GUS positive were eliminated. The GUS nega- tive T1 seeds were germinated, and the seedlings were ana- lyzed for the existence of antisense waxy gene. The transgenic T1 plants with only antisense waxy gene further were con- firmed through Southern blot analysis. The T2 seeds collected from marker-free T1 plants were used for amylose content analysis. The results showed that the amylose content in marker-free transgenic seeds reduced by up to 28.61% over the wild type rice. This marker-free transgenic rice with low amylose contents obtained in this project could be used to screen soft grain, and could also be used as a new rice re- source for eating quality improvement.
基金supported by the Important National Science & Technology Specific Projects(2010ZX03005-001-0)the Hi-Tech Research and Development of China(2006AA01Z272)the New Century Excellent Talents in University(NCET):(NCET-11-0593)
文摘In multi-cell cooperative multi-input multi-output （MIMO） systems, base station （BS） can exchange and utilize channel state information （CSI） of adjacent cell users to manage co-channel interference. Users quantize the CSIs of desired channel and interference channels using finite-rate feedback links, then BS can generate cooperative block diagonalization （BD） precoding matrices using the obtained quantized CSI at transmitter to supress co-channel interference. In this paper, a novel adaptive bit allocation scheme is proposed to minimize the rate loss due to imperfect CSI. We derive the closed-form expression of rate loss caused by both channel delay and limited feedback. Based on the derived rate loss expression, the proposed scheme can adaptively allocate more bits to quantize the better channels with smaller delays and fewer bits to worse channels with larger delays. Simulation results show that the proposed scheme yields higher performance than other allocation schemes.
