研究探讨了使用预训练的Pegasus模型进行长文本摘要时,不同文本分割方法对摘要质量的影响。收集来自知网的200篇关于STM32单片机的学术论文作为实验文本,比较了滑动窗口、句子分割、段落分割及滑动窗口加句子分割四种分割法的长文本摘...研究探讨了使用预训练的Pegasus模型进行长文本摘要时,不同文本分割方法对摘要质量的影响。收集来自知网的200篇关于STM32单片机的学术论文作为实验文本,比较了滑动窗口、句子分割、段落分割及滑动窗口加句子分割四种分割法的长文本摘要生成效果。实验使用ROUGE(Recall-Oriented Understudy for Gisting Evaluation)指标对生成的摘要进行评估,并对实验结果进行了详细分析。在生成摘要的质量方面,段落分割法表现出色,其ROUGE-1、ROUGE-2和ROUGE-L评分分别达到了30.85、7.60和20.15,轻微超过了句子分割法的评分,且显著优于句子分割加滑动窗口法。该研究旨在为研究者和开发者提供关于长文本摘要的实践经验和见解。展开更多
The transcription activator-like effector nuclease （TALEN） technique combined with the somatic cel nuclear transfer （SCNT） method has been successfuly applied for creating geneticaly modiifed pigs. However, method...The transcription activator-like effector nuclease （TALEN） technique combined with the somatic cel nuclear transfer （SCNT） method has been successfuly applied for creating geneticaly modiifed pigs. However, methods for isolating cels with bialelic indels requires further improvement because of the relatively low enrichment efifciency of mutated somatic cels. Moreover, little is known regarding the off-target effects of the TALEN system and the heredity of TALEN-modiifed pigs. In this study, an efifcient method to increase the enrichment efifciency of TALEN-mediated bialelic knockout （KO） cels was established, and corresponding geneticaly modiifed pigs with the expected genotype were generated whose off-target effect, fertility and heredity characteristics were aslo evaluated. Two TALEN pairs were constructed to target the porcine α-1,3-galactosyltransferase （GGTA1） gene locus. TALEN mRNA was transfected into the ear ifbroblasts folowed by the enrichment of α-Gal nul cels of minipigs using isolectin B4 （IB4） lectin and magnetic beads. A total of 115 cel colonies were formed and validated to beGGTA1 KO cels by sequencing and 10 bialelic KO cel colonies were used as nuclear donors for SCNT. ThirtyGGTA1 bialelic KO piglets were successfuly delivered and grew normaly. Seventeen potential off-target sites were investigated, and no off-target events were detected in the live piglets. To determine the fertility and heredity characteristics of TALEN-modiifed pigs, 10 mature founders were mated with each other and the mutations were determined to be transmitted to the F1 piglets. We established a robust and safe technology for developing geneticaly modiifed pig lines with expected genotypes for agricultural breeding and biomedical application.展开更多
文摘研究探讨了使用预训练的Pegasus模型进行长文本摘要时,不同文本分割方法对摘要质量的影响。收集来自知网的200篇关于STM32单片机的学术论文作为实验文本,比较了滑动窗口、句子分割、段落分割及滑动窗口加句子分割四种分割法的长文本摘要生成效果。实验使用ROUGE(Recall-Oriented Understudy for Gisting Evaluation)指标对生成的摘要进行评估,并对实验结果进行了详细分析。在生成摘要的质量方面,段落分割法表现出色,其ROUGE-1、ROUGE-2和ROUGE-L评分分别达到了30.85、7.60和20.15,轻微超过了句子分割法的评分,且显著优于句子分割加滑动窗口法。该研究旨在为研究者和开发者提供关于长文本摘要的实践经验和见解。
基金supported by the National Basic Research Program of China(973 Program)(2015CB554103 and 2011CBA01004)
文摘The transcription activator-like effector nuclease （TALEN） technique combined with the somatic cel nuclear transfer （SCNT） method has been successfuly applied for creating geneticaly modiifed pigs. However, methods for isolating cels with bialelic indels requires further improvement because of the relatively low enrichment efifciency of mutated somatic cels. Moreover, little is known regarding the off-target effects of the TALEN system and the heredity of TALEN-modiifed pigs. In this study, an efifcient method to increase the enrichment efifciency of TALEN-mediated bialelic knockout （KO） cels was established, and corresponding geneticaly modiifed pigs with the expected genotype were generated whose off-target effect, fertility and heredity characteristics were aslo evaluated. Two TALEN pairs were constructed to target the porcine α-1,3-galactosyltransferase （GGTA1） gene locus. TALEN mRNA was transfected into the ear ifbroblasts folowed by the enrichment of α-Gal nul cels of minipigs using isolectin B4 （IB4） lectin and magnetic beads. A total of 115 cel colonies were formed and validated to beGGTA1 KO cels by sequencing and 10 bialelic KO cel colonies were used as nuclear donors for SCNT. ThirtyGGTA1 bialelic KO piglets were successfuly delivered and grew normaly. Seventeen potential off-target sites were investigated, and no off-target events were detected in the live piglets. To determine the fertility and heredity characteristics of TALEN-modiifed pigs, 10 mature founders were mated with each other and the mutations were determined to be transmitted to the F1 piglets. We established a robust and safe technology for developing geneticaly modiifed pig lines with expected genotypes for agricultural breeding and biomedical application.
