Objective Pharmacological methods were used to screen targets and signaling pathways of Ma Xing Shi Gan Decoction(MXSGD)during influenza treatments,and mechanisms underlying antiinfluenza effects were elucidated.Metho...Objective Pharmacological methods were used to screen targets and signaling pathways of Ma Xing Shi Gan Decoction(MXSGD)during influenza treatments,and mechanisms underlying antiinfluenza effects were elucidated.Methods The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and relevant literature were searched under predefined conditions to identify the main compounds and their targets.Interactions between the target proteins were predicted using the STRING database.Gene Ontology(GO)functional enrichment analyses and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses were performed on the core targets involved in the influenza protein-protein interaction(PPI)network,using WebGestalt and the reactome database.iGEMDOCK was used for molecular docking of receptors and ligands to produce docking scores,and the results were visualized using Autodock and PyMOL.Results In total,126 major compounds and their respective targets were screened.355 influenza target proteins and 1221 influenza protein interactions were predicted using the STRING database.Influenza-related signaling pathways were strongly enriched in pharmacodynamic targets of MXSGD such as cytokine signaling in immune system and signaling by interleukin.The main biological process was response to the stimulates.Molecular docking results showed that RELALicochalcone A docking elicited by MXSGD,was superior to that of other target proteins and active compounds,suggesting that the docking site is also the main effector site of MXSGD during influenza treatments.Conclusions The results showed that MXSGD exerts antiinfluenza effects by interfering with virus adsorption,inhibiting virus proliferation,influencing immune functions and protecting host cells,which may prevent inflammation-induced tissue damage.展开更多
【目的】本研究旨在研究湿热环境中不同温度和风速对妊娠母猪的相关生化指标的影响。【方法】本实验选取年龄、体重相近、身体健康的妊娠母猪共20头,饲养于水帘风机猪舍,分别测定不同风速区间、温度中猪只的皮质醇(Cortistatin,CORT),...【目的】本研究旨在研究湿热环境中不同温度和风速对妊娠母猪的相关生化指标的影响。【方法】本实验选取年龄、体重相近、身体健康的妊娠母猪共20头,饲养于水帘风机猪舍,分别测定不同风速区间、温度中猪只的皮质醇(Cortistatin,CORT),丙二醛(Malondialdehyde,MDA),热应激蛋白70(Heat Shock Protein 70,HSP70)水平。【结果】风速、温度以及二者的互作效应能显著影响妊娠母猪血清中CORT的浓度(P<0.05);血清中MDA和HSP70的浓度受到环境参数的显著影响,当温度超过等热区时,随着温度升高,血清中MDA和HSP70浓度显著升高(P<0.05),风速对这二者的影响不显著(P>0.05)。【结论】该实验结果显示,在我国西南地区,湿热环境会影响猪只体内的生化指标,造成一定的热应激,合理的增强圈舍风速,降低舍内温度能够一定程度的降低妊娠母猪的热应激。展开更多
基金We thank for the funding support from the National Natural Science Foundation of China(No.81973670)the Natural Science Foundation of Hunan Province(No.2018JJ2297)+1 种基金the Key Program of Scientific Research Fund of Hunan Provincial Education Department(No.19A370)the Project of Research Learning and Innovative Experiment for College Students in Hunan(No.2016284,No.2016281,No.2017281and No.2018420).
文摘Objective Pharmacological methods were used to screen targets and signaling pathways of Ma Xing Shi Gan Decoction(MXSGD)during influenza treatments,and mechanisms underlying antiinfluenza effects were elucidated.Methods The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and relevant literature were searched under predefined conditions to identify the main compounds and their targets.Interactions between the target proteins were predicted using the STRING database.Gene Ontology(GO)functional enrichment analyses and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses were performed on the core targets involved in the influenza protein-protein interaction(PPI)network,using WebGestalt and the reactome database.iGEMDOCK was used for molecular docking of receptors and ligands to produce docking scores,and the results were visualized using Autodock and PyMOL.Results In total,126 major compounds and their respective targets were screened.355 influenza target proteins and 1221 influenza protein interactions were predicted using the STRING database.Influenza-related signaling pathways were strongly enriched in pharmacodynamic targets of MXSGD such as cytokine signaling in immune system and signaling by interleukin.The main biological process was response to the stimulates.Molecular docking results showed that RELALicochalcone A docking elicited by MXSGD,was superior to that of other target proteins and active compounds,suggesting that the docking site is also the main effector site of MXSGD during influenza treatments.Conclusions The results showed that MXSGD exerts antiinfluenza effects by interfering with virus adsorption,inhibiting virus proliferation,influencing immune functions and protecting host cells,which may prevent inflammation-induced tissue damage.
文摘【目的】本研究旨在研究湿热环境中不同温度和风速对妊娠母猪的相关生化指标的影响。【方法】本实验选取年龄、体重相近、身体健康的妊娠母猪共20头,饲养于水帘风机猪舍,分别测定不同风速区间、温度中猪只的皮质醇(Cortistatin,CORT),丙二醛(Malondialdehyde,MDA),热应激蛋白70(Heat Shock Protein 70,HSP70)水平。【结果】风速、温度以及二者的互作效应能显著影响妊娠母猪血清中CORT的浓度(P<0.05);血清中MDA和HSP70的浓度受到环境参数的显著影响,当温度超过等热区时,随着温度升高,血清中MDA和HSP70浓度显著升高(P<0.05),风速对这二者的影响不显著(P>0.05)。【结论】该实验结果显示,在我国西南地区,湿热环境会影响猪只体内的生化指标,造成一定的热应激,合理的增强圈舍风速,降低舍内温度能够一定程度的降低妊娠母猪的热应激。
