目的:探讨化痰活血益气方的抗心肌肥厚作用及对神经内分泌因子血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)、醛固酮(aldosterone,ALD)、内皮素-1(endothelin-1,ET-1)及Akt信号磷酸化水平的影响。方法:采用异丙肾上腺素(Isoproterenol,Iso)诱导...目的:探讨化痰活血益气方的抗心肌肥厚作用及对神经内分泌因子血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)、醛固酮(aldosterone,ALD)、内皮素-1(endothelin-1,ET-1)及Akt信号磷酸化水平的影响。方法:采用异丙肾上腺素(Isoproterenol,Iso)诱导的大鼠心肌肥厚模型(1mg/kg/d,s.c.,连续10d)。48只雄性SD大鼠随机分为正常组、模型组、复方低剂量组(4.88mg/kg/d)、复方中剂量组(9.77mg/kg/d)、复方高剂量组(19.53mg/kg/d)和卡托普利组(0.02mg/kg/d),每组8只。14 d相应药物干预后,测定左心室重量指数(left ventricle mass index,LVMI)、全心重量指数(heart mass index,HMI),ELISA法检测血清AngⅡ、ALD、ET-1的含量,Western blot法检测心肌组织Akt蛋白水平及其活性形式p-Akt蛋白水平的表达并计算p-Akt/Akt比率。结果:与正常组相比,模型组大鼠的LVMI、HMI、AngⅡ、ALD、ET-1和p-Akt/Akt比率均明显升高,差异均具有统计学意义(P<0.05)。与模型组相比,复方低、中、高剂量组与卡托普利组的LVMI、HMI、AngⅡ、ALD、ET-1和p-Akt/Akt比率均有不同程度的降低,复方中剂量组的上述各项指标始终存在显著性差异(P<0.05)。结论:①化痰活血益气方可以有效改善Iso诱导的心肌肥厚并降低神经内分泌因子AngⅡ、ALD、ET-1含量;②在各剂量组中,等效于成人剂量的中剂量组疗效最好,且该组疗效优于卡托普利组;③该复方可能通过抑制Akt信号的磷酸化来发挥其抗心肌肥厚作用。展开更多
Research has demonstrated that many chemical constituents dominated by piperidine alkaloids and flavonoids, such as lobelanidine, lobeline, and lobelanine, have been obtained from Lobelia chinensis Lour. Experimental ...Research has demonstrated that many chemical constituents dominated by piperidine alkaloids and flavonoids, such as lobelanidine, lobeline, and lobelanine, have been obtained from Lobelia chinensis Lour. Experimental studies and clinical applications have also indicated that L. chinensis possesses a number of pharmacological activities(e.g., diuretic, choleretic, breathing excitement, anti-venom, anti-bacterial, and anticancer). This paper focuses on the properties, chemical constituents, and anticancer activity of L. chinensis to clarify the connection among them, and identify the active anticancer compounds. This work serves as the foundation for further research and development of L. chinensis.展开更多
文摘目的:探讨化痰活血益气方的抗心肌肥厚作用及对神经内分泌因子血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)、醛固酮(aldosterone,ALD)、内皮素-1(endothelin-1,ET-1)及Akt信号磷酸化水平的影响。方法:采用异丙肾上腺素(Isoproterenol,Iso)诱导的大鼠心肌肥厚模型(1mg/kg/d,s.c.,连续10d)。48只雄性SD大鼠随机分为正常组、模型组、复方低剂量组(4.88mg/kg/d)、复方中剂量组(9.77mg/kg/d)、复方高剂量组(19.53mg/kg/d)和卡托普利组(0.02mg/kg/d),每组8只。14 d相应药物干预后,测定左心室重量指数(left ventricle mass index,LVMI)、全心重量指数(heart mass index,HMI),ELISA法检测血清AngⅡ、ALD、ET-1的含量,Western blot法检测心肌组织Akt蛋白水平及其活性形式p-Akt蛋白水平的表达并计算p-Akt/Akt比率。结果:与正常组相比,模型组大鼠的LVMI、HMI、AngⅡ、ALD、ET-1和p-Akt/Akt比率均明显升高,差异均具有统计学意义(P<0.05)。与模型组相比,复方低、中、高剂量组与卡托普利组的LVMI、HMI、AngⅡ、ALD、ET-1和p-Akt/Akt比率均有不同程度的降低,复方中剂量组的上述各项指标始终存在显著性差异(P<0.05)。结论:①化痰活血益气方可以有效改善Iso诱导的心肌肥厚并降低神经内分泌因子AngⅡ、ALD、ET-1含量;②在各剂量组中,等效于成人剂量的中剂量组疗效最好,且该组疗效优于卡托普利组;③该复方可能通过抑制Akt信号的磷酸化来发挥其抗心肌肥厚作用。
基金supported by the Macao Science and Technology Development Fund(No.102/2012/A3)the Research Fund of the University of Macao(No.MYRG 208(Y3-L4)-ICMS11-WYT)
文摘Research has demonstrated that many chemical constituents dominated by piperidine alkaloids and flavonoids, such as lobelanidine, lobeline, and lobelanine, have been obtained from Lobelia chinensis Lour. Experimental studies and clinical applications have also indicated that L. chinensis possesses a number of pharmacological activities(e.g., diuretic, choleretic, breathing excitement, anti-venom, anti-bacterial, and anticancer). This paper focuses on the properties, chemical constituents, and anticancer activity of L. chinensis to clarify the connection among them, and identify the active anticancer compounds. This work serves as the foundation for further research and development of L. chinensis.
