目的研究miRNA-34a、沉默信息调节因子(Silent information regulator,SIRT6)对老龄小鼠听觉皮层神经元细胞凋亡的影响及机制。方法miRNA-34a基因敲除小鼠(miRNA-34a敲除组)、SIRT6基因敲除小鼠(SIRT6敲除组)与野生型小鼠(对照组)各10只...目的研究miRNA-34a、沉默信息调节因子(Silent information regulator,SIRT6)对老龄小鼠听觉皮层神经元细胞凋亡的影响及机制。方法miRNA-34a基因敲除小鼠(miRNA-34a敲除组)、SIRT6基因敲除小鼠(SIRT6敲除组)与野生型小鼠(对照组)各10只,所有小鼠均为16月龄,TUNEL法分别检测各组小鼠听觉皮层神经元细胞凋亡情况,免疫组化法检测各组小鼠听觉皮层神经元中B细胞淋巴瘤-2(B-cell lymphoma-2,Bcl-2)、Bcl-2相关的x蛋白(B-cell lymphoma-2 associated X protein,Bax)、半胱氨酸天冬氨酸蛋白酶8(Cysteine aspartic protease8,Caspase8)、半胱氨酸天冬氨酸蛋白酶3(Cysteine aspartic protease3,Caspase3)表达情况。结果miRNA-34a敲除组小鼠听觉皮层神经元细胞凋亡指数与SIRT6组相比,差异有统计学意义(P<0.05),miRNA-34a敲除组与对照组相比,差异无统计学意义(P>0.05),SIRT6敲除组与对照组相比,差异有统计学意义(P<0.05);miRNA-34a敲除组小鼠听觉皮层神经元抑凋亡因子Bcl-2表达量与SIRT6敲除组相比,差异有统计学意义(P<0.05),miRNA-34a敲除组与对照组相比,差异无统计学意义(P>0.05),SIRT6敲除组与对照组相比,差异有统计学意义(P<0.05);miRNA-34a敲除组小鼠听觉皮层神经元促凋亡因子Bax、Caspase8、Caspase3表达量与SIRT6敲除组相比,差异有统计学意义(P<0.05),miRNA-34a敲除组与对照组相比,差异无统计学意义(P>0.05),SIRT6敲除组与对照组相比,差异有统计学意义(P<0.05)。结论miRNA-34a与SIRT6可通过多种凋亡因子调控老龄小鼠听觉皮层神经元细胞凋亡过程;miRNA-34a促进其凋亡,而SIRT6抑制小鼠听觉皮层神经元细胞凋亡;miRNA-34a与SIRT6在调控小鼠听觉皮层神经元凋亡中负相关,二者可能是治疗老年性耳聋的新靶点。展开更多
With the development of electric vehicles(EV), there is a huge demand for electric vehicle charging stations(EVCS). The utilization of renewable energy sources(RES) in EVCS can not only decrease the energy fluctuation...With the development of electric vehicles(EV), there is a huge demand for electric vehicle charging stations(EVCS). The utilization of renewable energy sources(RES) in EVCS can not only decrease the energy fluctuation by participating in peakload reduction of the grid, but also reduce the pollution to the environment by cutting down the use of fossil fuels. In this paper,the optimal planning for grid-connected EVCS with RES is studied by considering EV load uncertainty. Nine scenarios are set based on a different characteristic of EV load to reveal the impact of EV load on net present cost(NPC) and to express the relationship between the optimal capacity and energy flow. Moreover, since electricity price also plays an important role in EVCS planning, an economic comparison between different cases with different electricity prices for peak-valley-flat period is carried out. The results reveal the economic benefits of applying RES in EVCS, and demonstrate that EV load with different characteristics would influence the capacity of each device(PV, battery, converter) in the EVCS optimal planning.展开更多
基金supported by the International Science and Technology Cooperation Program of China(Grant No.2018YFE0125300)the National Natural Science Foundation of China(Grant No.52061130217)the Science and Technology Project of State Grid Hunan Electric Power Co.,Ltd.(Grant No.5216A2200005)。
文摘With the development of electric vehicles(EV), there is a huge demand for electric vehicle charging stations(EVCS). The utilization of renewable energy sources(RES) in EVCS can not only decrease the energy fluctuation by participating in peakload reduction of the grid, but also reduce the pollution to the environment by cutting down the use of fossil fuels. In this paper,the optimal planning for grid-connected EVCS with RES is studied by considering EV load uncertainty. Nine scenarios are set based on a different characteristic of EV load to reveal the impact of EV load on net present cost(NPC) and to express the relationship between the optimal capacity and energy flow. Moreover, since electricity price also plays an important role in EVCS planning, an economic comparison between different cases with different electricity prices for peak-valley-flat period is carried out. The results reveal the economic benefits of applying RES in EVCS, and demonstrate that EV load with different characteristics would influence the capacity of each device(PV, battery, converter) in the EVCS optimal planning.
