Objective:To investigate the relationships between GC gene polymorphisms and psoriasis vulgaris.Methods:A total of 101 patients with psoriasis vulgaris and 79 healthy controls were enrolled into this study,and they we...Objective:To investigate the relationships between GC gene polymorphisms and psoriasis vulgaris.Methods:A total of 101 patients with psoriasis vulgaris and 79 healthy controls were enrolled into this study,and they were all of Han nationality from Hainan province.The target gene capture sequencing method was used to sequence the full length of the GC gene and its 2kb upstream and downstream regions.SNP-based association analysis was performed under four genetic modes in SNPs with minimum allele frequency greater than 1%and the P value of Hardy-Weinberg equilibrium test in the control group is greater than 0.05.Bioinformatics methods were used to predict the impact of risk SNP on gene function.Results:A total of 94 SNPs were detected,of which 93 met the inclusion criteria.SNP-based association analyses showed that 21 SNPs(16 in introns,2 in exons,and 3 in Untranslated Regions)were susceptible to psoriasis vulgaris in at least one genetic mode(OR=0.289‑2.295,95%CI=0.048‑12.670,P<0.05).Bioinformatic prediction indicates that rs4588,located in the exon 11,was a non-synonymous mutation and can convert threonine to lysine(SIFT Score=0.481,SIFT Score Pred=T).rs4752 A>G located in the exon 8 was a synonymous mutation and did not cause amino acid change.Conclusion:GC gene is associated with the susceptibility of psoriasis vulgaris in Hainan Han ethnic group.展开更多
基金National Natural Science Foundation of China(No.81860551)Hainan Medical and Health Research Program(No.21A200466)。
文摘Objective:To investigate the relationships between GC gene polymorphisms and psoriasis vulgaris.Methods:A total of 101 patients with psoriasis vulgaris and 79 healthy controls were enrolled into this study,and they were all of Han nationality from Hainan province.The target gene capture sequencing method was used to sequence the full length of the GC gene and its 2kb upstream and downstream regions.SNP-based association analysis was performed under four genetic modes in SNPs with minimum allele frequency greater than 1%and the P value of Hardy-Weinberg equilibrium test in the control group is greater than 0.05.Bioinformatics methods were used to predict the impact of risk SNP on gene function.Results:A total of 94 SNPs were detected,of which 93 met the inclusion criteria.SNP-based association analyses showed that 21 SNPs(16 in introns,2 in exons,and 3 in Untranslated Regions)were susceptible to psoriasis vulgaris in at least one genetic mode(OR=0.289‑2.295,95%CI=0.048‑12.670,P<0.05).Bioinformatic prediction indicates that rs4588,located in the exon 11,was a non-synonymous mutation and can convert threonine to lysine(SIFT Score=0.481,SIFT Score Pred=T).rs4752 A>G located in the exon 8 was a synonymous mutation and did not cause amino acid change.Conclusion:GC gene is associated with the susceptibility of psoriasis vulgaris in Hainan Han ethnic group.
